Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,786,465 C→T G402G (GGC→GGT)  gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,786,4650CT97.8% 145.7 / NA 46G402G (GGC→GGT) gpmM2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/21);  minor base G (1/0);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGC  >  NC_000913/3786219‑3786708
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        
gATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACa                                                                                                                                                                                                                                                      <  1:10273/251‑1 (MQ=255)
  tGGCGCAAAATGACACAACTCAGTTGCGTATTTCTGAACCCGAAAAATATGCCCCCGTTACTTTCTTCTTCAACGGTGGCGTAGAAGCGTAGTTCAAAGGCGAAGATCGCATTCTGATCCACTCACCGAAAGTGGCTACCTCCGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATAAGACACCATTATCTGTAACTATCCGAACGGTGACATg                                                                                                                                                                                                                                                    >  2:286466/1‑251 (MQ=255)
     cGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGt                                                                                                                                                                                                                                                  >  1:84200/1‑250 (MQ=255)
          aaCGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAAAGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGGACGGGGACACGGTAGGTc                                                                                                                                                                                                                                             >  2:18440/1‑250 (MQ=255)
                            cGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAgc                                                                                                                                                                                                                          <  2:16879/251‑1 (MQ=255)
                                   ccgaaaccaaaaAATATTCCCACGTTACTTTCTTCTTCAACTGTGGCGTAGAAGAGTCGTGCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTTCGCAGAGCTGACCGAAAAACGGGGTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGGAACTATCCGAACGTTGACATGGTAGGTCACACCGGTGTGATGGAAGCGGCGGt                                                                                                                                                                                                                    <  2:358070/250‑1 (MQ=255)
                                     gaaaccgaaaAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTa                                                                                                                                                                                                                  >  2:424103/1‑250 (MQ=255)
                                             aaaaTATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTg                                                                                                                                                                                                        >  1:302189/1‑251 (MQ=255)
                                                  atGCCCACGTTACATTCTTCTTCAACGGTGGCGGAGAAGAGTCGTGCAAAGTCGAAGATCGCGTTCTGATCAACCCACCGAAAGTGGCTACCTACGAGCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCAGCAAGAGCGGCAAATACGACACCATGATCTGTAACTACCCGAACGTTGACATGGTAGGTCACCCCGGGGTGATGGGCCCGGCGGTTAAAG‑CGGTTTAAgca                                                                                                                                                                                                   <  2:248748/251‑2 (MQ=255)
                                                  aggtccACGTTACTTCCGTCTTCAACGGTTGCGTAGAAGAGTCTTTCAAAGGCGAAGATCGCATTCTGAGCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGTCAAATACGACACCATTATCTGTAACTATCCGAACGGTGAGATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTGAAAG‑CGGTTGacgga                                                                                                                                                                                                   <  2:424434/247‑5 (MQ=255)
                                                      ccACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTaaaaagggtgaagaactgg                                                                                                                                                                                                >  1:255655/1‑235 (MQ=255)
                                                        aCGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGAt                                                                                                                                                                                             <  1:383157/251‑1 (MQ=255)
                                                             aCTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATGATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTg                                                                                                                                                                                        <  1:286466/251‑1 (MQ=255)
                                                              cTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTg                                                                                                                                                                                                <  2:39692/242‑1 (MQ=255)
                                                              cTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGCACTgagatcggg                                                                                                                                                                                        >  1:39692/1‑242 (MQ=255)
                                                               tttCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGGTGAAGCACTGGAACACcgcg                                                                                                                                                                                      >  1:249538/1‑251 (MQ=255)
                                                                   ttcttcAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTCTCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑AGGTTggaacaacgggttactgcggggg                                                                                                                                                                                  >  1:238199/1‑229 (MQ=255)
                                                                   ttcttcAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGGCACACCGGGGTGCTGGAAGCGGCGGTTAAAG‑CGGGTGaaagactggagaactgcgtggg                                                                                                                                                                                  >  1:103653/1‑231 (MQ=255)
                                                                    tcttcAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTg                                                                                                                                                                                    <  1:413721/248‑1 (MQ=255)
                                                                     cttcAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑AGGGTGAAGCGCttgaactatgcgtggg                                                                                                                                                                                  >  2:187612/1‑234 (MQ=255)
                                                                                gCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGGAGGACTTGGTCACCGCGTGGAAGAAGTCGG‑Gaa                                                                                                                                                                     >  1:83782/1‑250 (MQ=255)
                                                                                  gTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGGTGAAGAAGTCGC‑GAAAGc                                                                                                                                                                  <  1:132391/251‑1 (MQ=255)
                                                                                                   aGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑AGGGTGGAGCACTGGATCACTTCGTGGGAAAAGTCGC‑GAAAGAGGATG‑AGTCCgtggggg                                                                                                                                                >  1:431999/1‑249 (MQ=255)
                                                                                                                        cAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCGCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACAACGGGGTGATGGAAGCGGCGGTTAAAG‑CGTTTGAAGCACTGGGTCACTGCGTGGAAGAATTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCt                                                                                                                            <  2:430556/250‑1 (MQ=255)
                                                                                                                          aCTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑CGGTTGGAACACTGGGTCACTGCGGGGAAGAAGACGC‑GAAAGCGGGTG‑GGTCCGTGGGGGGACAAATGCCGATCACCCCTgcc                                                                                                                         >  1:80870/1‑249 (MQ=255)
                                                                                                                                cGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑t                                                                                                                  <  2:171238/251‑1 (MQ=255)
                                                                                                                                 gAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATc                                                                                                                                  <  1:433248/235‑1 (MQ=255)
                                                                                                                                  aaaGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑Ta‑a                                                                                                               <  1:319441/251‑1 (MQ=255)
                                                                                                                                         ctacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGCACCGGGGCACTGCGTGGGAGAAGGCGC‑GGAAGGGGGTG‑AGGCCGGGGGGGGGCAAACGGTGATCACCGCTGGCCACGG‑GA‑ACGCTgg                                                                                                         >  2:75628/1‑249 (MQ=255)
                                                                                                                                          tacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGTTGTAGCACTCGGTCACTCAGTGGAGAAAGTGGC‑GAAAGAGGGTGTAGTC‑GGGGGGGGGCAACAGCTGATCACCGCCGCACACGG‑TA‑ACGCTGaa                                                                                                        >  2:14192/1‑249 (MQ=255)
                                                                                                                                           acctacGATCTGCAACCGGAAATGAGCCCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGaac                                                                                                       <  1:382749/250‑3 (MQ=255)
                                                                                                                                            cctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAg                                                                                                     <  1:187612/251‑1 (MQ=255)
                                                                                                                                             ctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGGTGGAGCACTGGATCACTGCGTGGACGAAGTCGC‑GCAAGCGGGTG‑AGGGCGGGGGTGGACAAATGCCGGTCCCCGCCCACCCAGG‑TA‑ACGCTGaaacg                                                                                                     >  2:118169/1‑246 (MQ=255)
                                                                                                                                                        aaCCGGAAATGAGCTCGGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAAACCGACACCTTTAGCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTGGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GATAGCGGTTG‑AGTCCGTGGGTGGACATCTGCTGATCACCGCTGTCCACGG‑TA‑ACGCTGATCAGATGCGCGATCCg                                                                                         <  2:434434/251‑1 (MQ=255)
                                                                                                                                                                                     gAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAggcacacacggcacacac                                                            <  2:83782/251‑1 (MQ=255)
                                                                                                                                                                                                    gCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGAGGCGCGATCCGGCAACGGGTCAGGCACACACGTCACACACCAACCTGCCAGTTcc                                             <  2:84200/251‑1 (MQ=255)
                                                                                                                                                                                                      cATCAAGAGCGGCAAATACGACACCATTATCTGAAACTATCCGAACCGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGAGGGTTGAA‑CACTTGATAACTGCGGGGGAACAGTGGG‑GACAGGGGTTG‑AGTCCGTGGGGGGAAAAATGATGGTTCCCGCTGAACACGG‑GA‑ACGCCGGACAGAAGCGCGCTCAGGCAAAGGGGCAGGGACACACGGCACACACCACCCCGCCAGTTCCGc                                           >  1:276338/1‑251 (MQ=255)
                                                                                                                                                                                                           agagCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTTAAG‑CGGTTGAAGCACTGGATCACTGCGTGGGAGACGTCGC‑GCACAAGGTTG‑GGTCCGTGGGGGGACAACCGCTGATCACAGCTGAACACGG‑GACAC‑CTGCAAAGCAGCGCGGTCCCGCAACGGGGCAGGCACACACGGCACACACCACCCtacccggtcccctggtt                                      >  2:391933/1‑235 (MQ=255)
                                                                                                                                                                                                           agagCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGAtt                                      <  2:302189/251‑1 (MQ=255)
                                                                                                                                                                                                           agagCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGTAGCACTGGATCACTTCGTGGGAGAAGTCGC‑GCAAGCGGGTG‑AGTCCGTGGGTGGGGAACTGCTGGTCACCGCCGACCACGG‑GA‑ACGCTGGACAGATGGGCGGTCCGGGCACGGGGCAggcacacaccgcacacacacacccgccccttcccccgatt                                      >  2:221403/1‑229 (MQ=255)
                                                                                                                                                                                                                    aaTACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTTAAG‑CGGGTGAAGCACTGGATCACTGCGGGGGAGAAGTCGC‑GGAAGAGGTTG‑AGTCCGTGGGGGGGCAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGGAAAGATGCGCGCTCCGGCAACGGGGCAGGCACACACGGCACCCACCAACCCGCCAGTTCCGCTGATTTTCGtttgt                             >  1:102222/1‑248 (MQ=255)
                                                                                                                                                                                                                        cGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑CGGATGTAGCCCTGGATCCCTGCGTGGGAGCAGGCGCAGAAA‑AGGGTG‑AGTACGCGGGTGGGAAAATGCTGATACCCGCTGACCACGGGTA‑A‑GCTGCACAGGTGCGCGGTCCGGCCACGGGGCCAGCACACACCGCAACCACCACCATGCCACCTCCCCTGATTTACGtgtgtggt                          >  2:224347/1‑243 (MQ=255)
                                                                                                                                                                                                                              cattatcTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGCACTGGATCACTGCGGGGGAGAAGACGC‑GGAAGCGGGTG‑AGTGCGGGGGGGGGCAACTGCTGGTCACCGCCGACCCCGG‑GA‑ACGGTGGACAGATGCGCGATCCGGCCACGGGGCAGGGACACACGGCCCACACCAAACCGCCAGTTCCGCTGGTTTACGGTGGGGATAAGaa                     >  1:332335/5‑249 (MQ=255)
                                                                                                                                                                                                                                 tatcTGTAACTAGCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTg                 <  2:332335/249‑1 (MQ=255)
                                                                                                                                                                                                                                          cTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGCTGAAGCACCGGGTCAATGCGGGGGAGAAGGGGC‑GAAAACGGTGG‑AGTCCGGGGGGGGACAAATGCTGCTCACCGCTGACCACGG‑TA‑AAGCCGAACAGATGCGCGCTCCGGCAAAGGGTCCGGGACACCCGGCGCACACCCACCTCCCAGTTCCGCCGATTTTCGTTGGTGATAAGAAAGTGaaaaaggttg       >  1:43035/1‑244 (MQ=255)
                                                                                                                                                                                                                                               cGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGATAAAG‑CGGGTGAAGCACTGGGTAACTGCGGGGGAGAAGTCGC‑GGAAGAGGGTG‑GGTCCGGGGGGGGGCAACTGCTGATAACCGCTGGCCACGG‑GA‑AAGCTGAACAGATGCGCGATACGGCCACGGGGCCGGCACACACGGCACACCCCCACCCGCCCGTTCCCCCGGTTTAAGTTGGTGATaaaaaaggggaaacgggtgcaggg  >  1:203933/1‑229 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        
GATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGC  >  NC_000913/3786219‑3786708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑