Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,988	T→A	A243A (GCT→GCA)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,988	0	T	A	100.0%	182.8 / NA	52	A243A (GCT→GCA)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (26/26);  total (26/26)

GTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAC  >  NC_000913/3785741‑3786234                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGaa                                                                                                                                                                                                                                                     <  1:392443/251‑1 (MQ=255)             cGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGcc                                                                                                                                                                                                                                             >  1:310455/1‑247 (MQ=255)                        tttgaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATgg                                                                                                                                                                                                                               >  1:116837/4‑250 (MQ=255)                          tgaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGaa                                                                                                                                                                                                                             <  2:124029/249‑1 (MQ=255)                           gaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGTCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGa                                                                                                                                                                                                                              >  1:434434/1‑248 (MQ=255)                            aagagAAATGTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGAc                                                                                                                                                                                                                          <  2:123910/251‑1 (MQ=255)                               agaaaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGc                                                                                                                                                                                                                       <  2:349473/249‑1 (MQ=255)                                  aaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGAt                                                                                                                                                                                                                    >  1:7983/1‑251 (MQ=255)                                     ttGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGc                                                                                                                                                                                                                  <  2:118697/250‑1 (MQ=255)                                       gctgcgcAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGcgct                                                                                                                                                                                                               >  1:86738/4‑248 (MQ=255)                                            gcagggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGAttt                                                                                                                                                                                                          <  1:431139/244‑1 (MQ=255)                                               gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTc                                                                                                                                                                                                        >  1:353062/5‑250 (MQ=255)                                                    aaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAAc                                                                                                                                                                                                  >  1:104220/1‑251 (MQ=255)                                                       gCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTc                                                                                                                                                                                               >  1:301032/1‑251 (MQ=255)                                                       gCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTc                                                                                                                                                                                               <  2:104220/251‑1 (MQ=255)                                                        ccGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTcc                                                                                                                                                                                              >  2:10273/1‑251 (MQ=255)                                                              tgcttCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCt                                                                                                                                                                                         <  1:182789/246‑1 (MQ=255)                                                                    catcatTCGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCAGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGCCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACcgcg                                                                                                                                                                                  >  2:373996/1‑251 (MQ=255)                                                                       catTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCGGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGGCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACcgcgcg                                                                                                                                                                                <  2:373766/250‑1 (MQ=255)                                                                                  actacGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACt                                                                                                                                                                    <  1:268832/251‑1 (MQ=255)                                                                                   ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTc                                                                                                                                                                   >  2:335382/1‑251 (MQ=255)                                                                                         gATGGACCGCGATAACCGTTGGGATCGCTTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGcat                                                                                                                                                             <  2:336798/251‑3 (MQ=255)                                                                                            ggACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATTGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCg                                                                                                                                                          <  1:58041/251‑1 (MQ=255)                                                                                                          gTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTAGTCGTGCAGAAGGTCAGCCAGATGCGGCAAGGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAGCGCAGATTTc                                                                                                                                            <  1:69855/251‑1 (MQ=255)                                                                                                                  cgcgTTGAAAAAGCCTATGCCCTGCTGACGCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCt                                                                                                                                     <  1:375823/250‑1 (MQ=255)                                                                                                                        gAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTcgcgcg                                                                                                                              <  1:59556/251‑1 (MQ=255)                                                                                                                           aaaGCATATGACCCGCTGACCCTGGCGCAGGGTGTGTTCCAGGCCGATACTGCCATTCCTAGCTTACAGGCAGCTTATGCCCACGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCCCGCGTa                                                                                                                            >  2:205816/1‑250 (MQ=255)                                                                                                                                    gACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGt                                                                                                                  >  2:367932/1‑251 (MQ=255)                                                                                                                                      ccTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTa                                                                                                                <  1:402336/251‑1 (MQ=255)                                                                                                                                         gctgACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACg                                                                                                             >  1:56504/1‑251 (MQ=255)                                                                                                                                                     gcAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGtt                                                                                                                 >  1:44168/1‑235 (MQ=255)                                                                                                                                                     gcAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACGCGTGCATTCGTAAACGCAGATTTCGACGGCTGCGCGCGTAAGAAAGTGGtt                                                                                                                 <  2:44168/235‑1 (MQ=255)                                                                                                                                                      cAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGAt                                                                                                >  2:240519/1‑251 (MQ=255)                                                                                                                                                         ggtgAGTTCCAGGCCGATACGGCCGTTGCTGGTGTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCt                                                                                             <  2:67226/248‑1 (MQ=255)                                                                                                                                                                 ccAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAAt                                                                                     <  1:130203/251‑1 (MQ=255)                                                                                                                                                                     gCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAcgc                                                                                 >  1:345565/1‑251 (MQ=255)                                                                                                                                                                           actgccgTTGCTGGTTTGCAGGCAGCTTATGCTAGCGACGACAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTgg                                                                           >  2:413721/4‑250 (MQ=255)                                                                                                                                                                              gccgTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACAt                                                                        >  1:197754/1‑251 (MQ=255)                                                                                                                                                                                                 cagctTATGCGCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGGCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTac                                                     <  2:310455/249‑1 (MQ=255)                                                                                                                                                                                                  agctTATGCTCGCGACGAAAAAGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGAAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacc                                                    >  1:358070/2‑251 (MQ=255)                                                                                                                                                                                                  agctTATGCGCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacc                                                    <  1:373996/250‑1 (MQ=255)                                                                                                                                                                                                   gctTATGCTCGCGTCGAAAACGACGAGTTCGTGAAAGCGACCGTGATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGGTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGTTTAACGTCGATTTCGTTATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccc                                                   <  1:233891/251‑1 (MQ=255)                                                                                                                                                                                                      tATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccca                                                  <  1:335382/249‑1 (MQ=255)                                                                                                                                                                                                              cgacgaAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTccc                                        >  1:377150/1‑251 (MQ=255)                                                                                                                                                                                                                      acgacgagttCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAac                                >  1:16879/9‑251 (MQ=255)                                                                                                                                                                                                                          cgagttCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGCTGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGGAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGAGGTTAACGTCGATTTCGTGATGCTGACCGAATAAGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCt                            >  1:248748/5‑251 (MQ=255)                                                                                                                                                                                                                               tCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCgg                        <  1:209280/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTgg                 >  2:214315/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCCGTTATGCGTGCAGAAGGTCAGCCAGATGCGGCAATGGTAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCTCGGACAGCAAAACTGCGGTTGCTGACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGGGGATGGc            <  2:58121/251‑1 (MQ=255)                                                                                                                                                                                                                                            cGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACAGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACCTCACAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCg           >  1:361299/1‑250 (MQ=255)                                                                                                                                                                                                                                             ggtattcGTGCAGAAGGGCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGAGTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGATTCGCGCGTAAGAAAGGTGTTAACGTCGATTTCGTGATGCTTACCGAATGCGCCGCTGACAGCAAAACGGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGTATGGCGaa         <  2:216167/245‑1 (MQ=255)                                                                                                                                                                                                                                                    gTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc  >  2:23592/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAC  >  NC_000913/3785741‑3786234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑