Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,958	T→C	D233D (GAT→GAC)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,958	0	T	C	100.0%	167.5 / NA	48	D233D (GAT→GAC)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

GTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCC  >  NC_000913/3785720‑3786196                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               gTCGCGACACCCCTCCGCGCTGTGCTGAATCCTCGCTGAAAAAATTTGAAGATAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTTAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTTAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGaat                                                                                                                                                                                                                                    <  2:208729/251‑2 (MQ=255)       acacCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGAcc                                                                                                                                                                                                                              <  2:122191/251‑1 (MQ=255)           cccgccgCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTa                                                                                                                                                                                                                          <  1:414288/250‑1 (MQ=255)                cgcgCAGTGCTAAATACTCGCTGAAACACTTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGACGCGTAGCGTACATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAACACGACGGGTTCGTGAAAGCGACCGTTAttcgg                                                                                                                                                                                                                     >  2:382749/1‑247 (MQ=255)                      gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGaa                                                                                                                                                                                                               <  1:392443/251‑1 (MQ=255)                                  cGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGcc                                                                                                                                                                                                       >  1:310455/1‑247 (MQ=255)                                             tttgaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATgg                                                                                                                                                                                         >  1:116837/4‑250 (MQ=255)                                               tgaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGaa                                                                                                                                                                                       <  2:124029/249‑1 (MQ=255)                                                gaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGTCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGa                                                                                                                                                                                        >  1:434434/1‑248 (MQ=255)                                                 aagagAAATGTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGAc                                                                                                                                                                                    <  2:123910/251‑1 (MQ=255)                                                    agaaaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGc                                                                                                                                                                                 <  2:349473/249‑1 (MQ=255)                                                       aaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGAt                                                                                                                                                                              >  1:7983/1‑251 (MQ=255)                                                          ttGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGc                                                                                                                                                                            <  2:118697/250‑1 (MQ=255)                                                            gctgcgcAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGcgct                                                                                                                                                                         >  1:86738/4‑248 (MQ=255)                                                                 gcagggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGAttt                                                                                                                                                                    <  1:431139/244‑1 (MQ=255)                                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTc                                                                                                                                                                  >  1:353062/5‑250 (MQ=255)                                                                         aaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAAc                                                                                                                                                            >  1:104220/1‑251 (MQ=255)                                                                            gCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTc                                                                                                                                                         >  1:301032/1‑251 (MQ=255)                                                                            gCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTc                                                                                                                                                         <  2:104220/251‑1 (MQ=255)                                                                             ccGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTcc                                                                                                                                                        >  2:10273/1‑251 (MQ=255)                                                                                   tgcttCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCt                                                                                                                                                   <  1:182789/246‑1 (MQ=255)                                                                                         catcatTCGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCAGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGCCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACcgcg                                                                                                                                            >  2:373996/1‑251 (MQ=255)                                                                                            catTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCGGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGGCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACcgcgcg                                                                                                                                          <  2:373766/250‑1 (MQ=255)                                                                                                       actacGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACt                                                                                                                              <  1:268832/251‑1 (MQ=255)                                                                                                        ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTc                                                                                                                             >  2:335382/1‑251 (MQ=255)                                                                                                              gATGGACCGCGATAACCGTTGGGATCGCTTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGcat                                                                                                                       <  2:336798/251‑3 (MQ=255)                                                                                                                 ggACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATTGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCg                                                                                                                    <  1:58041/251‑1 (MQ=255)                                                                                                                               gTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTAGTCGTGCAGAAGGTCAGCCAGATGCGGCAAGGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAGCGCAGATTTc                                                                                                      <  1:69855/251‑1 (MQ=255)                                                                                                                                       cgcgTTGAAAAAGCCTATGCCCTGCTGACGCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCt                                                                                               <  1:375823/250‑1 (MQ=255)                                                                                                                                             gAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTcgcgcg                                                                                        <  1:59556/251‑1 (MQ=255)                                                                                                                                                aaaGCATATGACCCGCTGACCCTGGCGCAGGGTGTGTTCCAGGCCGATACTGCCATTCCTAGCTTACAGGCAGCTTATGCCCACGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCCCGCGTa                                                                                      >  2:205816/1‑250 (MQ=255)                                                                                                                                                         gACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGt                                                                            >  2:367932/1‑251 (MQ=255)                                                                                                                                                           ccTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTa                                                                          <  1:402336/251‑1 (MQ=255)                                                                                                                                                              gctgACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACg                                                                       >  1:56504/1‑251 (MQ=255)                                                                                                                                                                          gcAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGtt                                                                           >  1:44168/1‑235 (MQ=255)                                                                                                                                                                          gcAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACGCGTGCATTCGTAAACGCAGATTTCGACGGCTGCGCGCGTAAGAAAGTGGtt                                                                           <  2:44168/235‑1 (MQ=255)                                                                                                                                                                           cAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGAt                                                          >  2:240519/1‑251 (MQ=255)                                                                                                                                                                              ggtgAGTTCCAGGCCGATACGGCCGTTGCTGGTGTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCt                                                       <  2:67226/248‑1 (MQ=255)                                                                                                                                                                                      ccAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAAt                                               <  1:130203/251‑1 (MQ=255)                                                                                                                                                                                          gCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAcgc                                           >  1:345565/1‑251 (MQ=255)                                                                                                                                                                                                actgccgTTGCTGGTTTGCAGGCAGCTTATGCTAGCGACGACAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTgg                                     >  2:413721/4‑250 (MQ=255)                                                                                                                                                                                                   gccgTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACAt                                  >  1:197754/1‑251 (MQ=255)                                                                                                                                                                                                                      cagctTATGCGCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGGCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTac               <  2:310455/249‑1 (MQ=255)                                                                                                                                                                                                                       agctTATGCTCGCGACGAAAAAGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGAAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacc              >  1:358070/2‑251 (MQ=255)                                                                                                                                                                                                                       agctTATGCGCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacc              <  1:373996/250‑1 (MQ=255)                                                                                                                                                                                                                        gctTATGCTCGCGTCGAAAACGACGAGTTCGTGAAAGCGACCGTGATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGGTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGTTTAACGTCGATTTCGTTATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccc             <  1:233891/251‑1 (MQ=255)                                                                                                                                                                                                                           tATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccca            <  1:335382/249‑1 (MQ=255)                                                                                                                                                                                                                                   cgacgaAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTccc  >  1:377150/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               GTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCC  >  NC_000913/3785720‑3786196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑