Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,069,743 C→T A33T (GCA→ACA)  rutG ← pyrimidine:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,069,7430CT95.2% 125.9 / ‑6.3 43A33T (GCA→ACA) rutGpyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (30/10);  total (30/13)
Fisher's exact test for biased strand distribution p-value = 7.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGC  >  NC_000913/1069515‑1069978
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           
tatTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGCCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGt                                                                                                                                                                                                                       >  1:427288/1‑251 (MQ=255)
          aaTGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAACCAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGgcg                                                                                                                                                                                                             >  1:284027/1‑251 (MQ=255)
                      gTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCAcgccgc                                                                                                                                                                                                 >  1:27135/1‑251 (MQ=255)
                                gTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGt                                                                                                                                                                                          >  2:329761/1‑248 (MQ=255)
                                     ggcgATAACCACGACGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAAGCACCCGACGGTAATAAAGCAAAACAGTAACGTGCCGATCCCCGACATTCAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACCCCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGCGCAAACGGCAATCGTTCATCCGGCGCGACCCCGCCGCTCTCTGTagggtccg                                                                                                                                                                                  >  2:414591/1‑245 (MQ=255)
                                      gcgATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCg                                                                                                                                                                                  >  1:275257/1‑250 (MQ=255)
                                                 gCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGcc                                                                                                                                                                      >  1:138164/1‑251 (MQ=255)
                                                              gcgcTGGAGCCGAGATAACTGGGAACGCGCCCGCCGGTAATACAGCAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCCTCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGCCGCGACCACGCCGCTCTCTGTAGAGGTCGACTTTAACTGCCAGTGAGGAAAAcc                                                                                                                                                         >  2:402592/1‑251 (MQ=255)
                                                                                cTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGCTCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATAGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAAACGAACATTGCCATCAGCTg                                                                                                                                       >  1:257997/1‑251 (MQ=255)
                                                                                cTGGGGACACGCCCGCCGGTCATAAAGAAAAACAGTAACGTGCCGATCCCCGCCATTAAAATGGAAAGATTGGGATACAGCCACATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCtt                                                                                                                                       >  2:206449/1‑250 (MQ=255)
                                                                                 tGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATAAGCTGt                                                                                                                                      >  2:328588/1‑251 (MQ=255)
                                                                                  ggggACGCCCCGGCGGGAAATAAAGAAAAACATAAACGTGCCGACCCCCGACATTAAAATGGAAAGATAGAGATCCAGCCCCTTCAGAATCGACAGTAACCCCGTCGCGCCAAGCATCGCCTCCGCGTGTTGAACGCCCATTGCTGTCGTTTGAGCAAACGGCATTGGTTCATCCGGCGCAGCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGtc                                                                                                                                     <  2:24594/251‑1 (MQ=255)
                                                                                            ccgccgGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAACTGGAAAGATTAAGATCCCGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGAACACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCAAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAagg                                                                                                                            >  2:42035/1‑250 (MQ=255)
                                                                                               ccgGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGt                                                                                                                        >  1:231751/1‑251 (MQ=255)
                                                                                               ccgGTAAAAAAGAAAAACAGAAACGGGCGGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACCCCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGt                                                                                                                        <  2:138164/251‑1 (MQ=255)
                                                                                                 ggTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGtt                                                                                                                       >  1:85861/1‑250 (MQ=255)
                                                                                                       aaaGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGc                                                                                                                <  1:234885/251‑1 (MQ=255)
                                                                                                                  cccgatctccGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCa                                                                                                     <  1:25664/243‑1 (MQ=255)
                                                                                                                   tAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATc                                                                                                      >  1:157674/1‑249 (MQ=255)
                                                                                                                          ccGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCa                                                                                                     >  2:25664/1‑243 (MQ=255)
                                                                                                                          ccGACCCCCGACATAAAAATGAAAAGATTGGGATCCAGCCCCATCAGAATCGGCATAAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACGGTCGTCTGAGCAAACGGCAATCGTGCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGATGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGa                                                                                             <  1:264657/251‑1 (MQ=255)
                                                                                                                                          aaaTGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAAcc                                                                             >  1:320080/1‑251 (MQ=255)
                                                                                                                                          aaaTGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAAcc                                                                             >  1:395660/1‑251 (MQ=255)
                                                                                                                                                 aaGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACAcc                                                                        >  1:401807/1‑249 (MQ=255)
                                                                                                                                                  aGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACCTCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACAACGACGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGc                                                                     >  1:386968/1‑251 (MQ=255)
                                                                                                                                                  aGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAagg                                                                                                                            >  1:283442/1‑196 (MQ=255)
                                                                                                                                                    aTTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGccc                                                                   <  2:27135/251‑1 (MQ=255)
                                                                                                                                                        ggATCCAGCCCCATCGGAAGCGGCATTAACACCGTCGCGCCAAACACCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAg                                                               <  1:40203/251‑1 (MQ=255)
                                                                                                                                                         gATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAgg                                                              >  1:82678/1‑251 (MQ=255)
                                                                                                                                                          aTCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTTTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAggg                                                             >  1:322374/1‑251 (MQ=255)
                                                                                                                                                           tCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAgggg                                                            >  1:401209/1‑250 (MQ=255)
                                                                                                                                                                           tCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGAGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGGTGa                                             >  1:8121/1‑250 (MQ=255)
                                                                                                                                                                            cGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAAt                                           >  1:122800/1‑251 (MQ=255)
                                                                                                                                                                                   aaccccGTCGCGCCAAACATCGCCACCGCGTGTGGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCg                                    <  1:146029/247‑1 (MQ=255)
                                                                                                                                                                                        cGTCGCTCCAAACACCGCCCCCGCGTGTGGAACGCCCAGTACTGTCGGCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGgc                               <  1:220446/251‑1 (MQ=255)
                                                                                                                                                                                         gTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGgcg                              >  1:225964/1‑251 (MQ=255)
                                                                                                                                                                                               ccAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAAc                        <  1:141635/251‑1 (MQ=255)
                                                                                                                                                                                               ccAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAAc                        >  2:288746/1‑251 (MQ=255)
                                                                                                                                                                                                    cctcccccccGCGTGTGGACCGCCCTTTACGGGCGTCTGAGCAACCGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAAt                   <  2:401807/243‑1 (MQ=255)
                                                                                                                                                                                                              gtgtgtTGAACGCCAATGCCGGCCGTCTGAGCAAACGGCAATGGTTCATCGGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTg         <  2:401209/249‑1 (MQ=255)
                                                                                                                                                                                                              gcgTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGGGATAACTGCGATCGAACCACACCAGCCCGTAGGGGGTGGGGTGACGATGAATCGCTTCGATGGCGCAACACAGAATGTCGTGGGTg         >  2:334316/1‑251 (MQ=255)
                                                                                                                                                                                                                gttttGAACGCCCATTACTGTCGTCTGAGCAAACTGCAATCGTTCATCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGcc       <  2:85861/248‑1 (MQ=255)
                                                                                                                                                                                                                     gAACGCCAATTACTGTCGTCAGAGCAAACTGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTATAGAGGTCGATTTTAACTGCCCGTGAGGAAAACCGACAATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGCTGGAGCAGAACAGCATGAAGTGGGTGCCGACGc  >  2:29035/1‑251 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           
TATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGC  >  NC_000913/1069515‑1069978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑