Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,786	T→A	L176Q (CTG→CAG)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,786	0	T	A	100.0%	203.6 / NA	62	L176Q (CTG→CAG)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (31/31);  total (31/31)

TGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACT  >  NC_000913/3785541‑3786029                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    tGCTAAACCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAgggt                                                                                                                                                                                                                                                 <  2:77194/250‑2 (MQ=255)         cagTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCcg                                                                                                                                                                                                                                         >  1:316434/3‑250 (MQ=255)         cagTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCcg                                                                                                                                                                                                                                         <  2:316434/248‑1 (MQ=255)           gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGAAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGCTGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGACGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAACAATTTGAAGAGAAATTTGCTGCGCAGGGAAAAGGCCGCGt                                                                                                                                                                                                                                      >  2:375823/1‑251 (MQ=255)               tGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCg                                                                                                                                                                                                                                  >  2:122332/1‑251 (MQ=255)                    ggCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTcc                                                                                                                                                                                                                               <  2:117355/249‑1 (MQ=255)                                aaaGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGtt                                                                                                                                                                                                                  >  1:408418/1‑250 (MQ=255)                                  aGCGAAAAACGCAGGGACAACAGTACACATTATAGGTCTGCTATCCGCAGGCGACGTACACAGCCCCGAAGATCACATCATGCCGATGGTAGAACTGGCAGATGAACGCGGCGCAGAAAAAATCTACCTGCACGAATTCATTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCAATCATTGGTCGTtact                                                                                                                                                                                                               >  2:69855/1‑251 (MQ=255)                                         aaCGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGAt                                                                                                                                                                                                        >  2:3917/1‑251 (MQ=255)                                          aCGCAGGTAAAGCAGAACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATTGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCTCCGCGCAGTTCTGAATCCTCGCTGAAAAAATTTGAAGATAAAGTTGCCGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGGTACTACGCGATg                                                                                                                                                                                                       <  2:262234/251‑1 (MQ=255)                                           cGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGATGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATgg                                                                                                                                                                                                      <  1:406285/251‑1 (MQ=255)                                            gCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGACCTGGCAGCTGAACGCGGCGCAGAACAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGAAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATgg                                                                                                                                                                                                      >  1:424434/1‑250 (MQ=255)                                               ggtaaaGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACcg                                                                                                                                                                                                  <  2:329258/248‑1 (MQ=255)                                                  aaaGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACcgcg                                                                                                                                                                                                >  1:349473/1‑250 (MQ=255)                                                          acacATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGtt                                                                                                                                                                                       <  1:222820/251‑1 (MQ=255)                                                                tATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATc                                                                                                                                                                                 >  2:431139/1‑251 (MQ=255)                                                                  tGGGTCTGCTCTACGCAGGCGGCGTACACAGCTACGAAGATTACATCATGGCGCTGGAAGAACTGGCAGCTGAACGCGGCGCAGCAAAAATCTACCTGCACGCATTCCTTGATGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAACAATTTGAAGAGAAATTTGCTGCGCAGGGAAAAGGCCGAGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGAGATAACCGTTGGGATcgc                                                                                                                                                                               >  1:101398/1‑251 (MQ=255)                                                                       cTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCCGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTACAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGttg                                                                                                                                                                           >  1:58121/1‑248 (MQ=255)                                                                         gCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGttg                                                                                                                                                                           <  1:109075/248‑3 (MQ=255)                                                                         gCTCCCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACGGGCAGCTGAACGCGGCGCAGAAAAAAGCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGGTGGGATCGCGttg                                                                                                                                                                           <  2:101811/248‑3 (MQ=255)                                                                              ccGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGc                                                                                                                                                                    <  1:3917/250‑1 (MQ=255)                                                                                         gTACACAGCCACGAAGAACACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATGTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACctgct                                                                                                                                                        <  2:408418/251‑1 (MQ=255)                                                                                           acacAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGaaaaa                                                                                                                                                                      <  2:87057/235‑1 (MQ=255)                                                                                           acacAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGaaaaa                                                                                                                                                                      >  1:87057/1‑235 (MQ=255)                                                                                            cacaGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGAc                                                                                                                                                     >  2:402336/1‑251 (MQ=255)                                                                                                 ccACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTgg                                                                                                                                                >  1:336798/1‑251 (MQ=255)                                                                                                   aCGAAGATCACATCAGGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGgcg                                                                                                                                              <  2:338789/251‑1 (MQ=255)                                                                                                      aaGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGgcgc                                                                                                                                             >  1:124029/1‑249 (MQ=255)                                                                                                      aaGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGgcgc                                                                                                                                             >  1:118697/1‑249 (MQ=255)                                                                                                          tCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAgggtg                                                                                                                                       >  2:209280/1‑249 (MQ=255)                                                                                                                catGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCTCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTc                                                                                                                                  <  1:64981/250‑1 (MQ=255)                                                                                                                               aCTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCAGTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATActgccg                                                                                                                  <  1:359389/251‑1 (MQ=255)                                                                                                                                  ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGCTACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCAGTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTg                                                                                                               <  1:205320/251‑1 (MQ=255)                                                                                                                                  ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGtt                                                                                                                <  1:139444/250‑1 (MQ=255)                                                                                                                                  ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTAGACGGACGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAACGGCCGCGTAGCATCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGAAGGGTGAGTTCCAGGCCGCTACTGCCGTTg                                                                                                               >  1:253967/1‑251 (MQ=255)                                                                                                                                         gAACGCGGCGCAGAAAAAATCTACCTGCACGCATCCCTTGACGGTCGCGACAACCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACTCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGTGTGAGTTCCAGGCCGATACTGCCGTTGCTGGttt                                                                                                        <  1:107959/251‑1 (MQ=255)                                                                                                                                          aaCGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTg                                                                                                       >  1:67226/1‑251 (MQ=255)                                                                                                                                                    aGAAAAAAGCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTGCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCtt                                                                                             <  2:278536/251‑1 (MQ=255)                                                                                                                                                     gAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGACCAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACAGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCtt                                                                                             >  2:66010/1‑250 (MQ=255)                                                                                                                                                      aaaaaaTCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTAGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTAt                                                                                           >  2:95167/1‑251 (MQ=255)                                                                                                                                                        aaaaTCTACCTGCACGCATTCATTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGAGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGAAGGCAGCTTATGc                                                                                         >  2:383157/1‑251 (MQ=255)                                                                                                                                                           aTCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAcc                                                                                                                                                                                          <  2:358843/151‑1 (MQ=255)                                                                                                                                                           aTCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAcc                                                                                                                                                                                          >  1:358843/1‑151 (MQ=255)                                                                                                                                                               aCCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTcgct                                                                                    <  2:275578/249‑2 (MQ=255)                                                                                                                                                                    cACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGaaa                                                                              >  2:130203/1‑250 (MQ=255)                                                                                                                                                                        cATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCGCGCGACGaaaacgac                                                                         <  1:122332/251‑5 (MQ=255)                                                                                                                                                                        cATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGACCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGaaaacgac                                                                         <  1:321877/251‑5 (MQ=255)                                                                                                                                                                         aTTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGACATTTGCTGCGCAGGGAAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGaaaacgacg                                                                        >  1:297514/1‑246 (MQ=255)                                                                                                                                                                                    gTCGCGACACCCCTCCGCGCTGTGCTGAATCCTCGCTGAAAAAATTTGAAGATAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTTAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTTAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGaat                                                             <  2:208729/251‑2 (MQ=255)                                                                                                                                                                                          acacCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGAcc                                                       <  2:122191/251‑1 (MQ=255)                                                                                                                                                                                              cccgccgCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTa                                                   <  1:414288/250‑1 (MQ=255)                                                                                                                                                                                                   cgcgCAGTGCTAAATACTCGCTGAAACACTTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGACGCGTAGCGTACATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAACACGACGGGTTCGTGAAAGCGACCGTTAttcgg                                              >  2:382749/1‑247 (MQ=255)                                                                                                                                                                                                         gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGaa                                        <  1:392443/251‑1 (MQ=255)                                                                                                                                                                                                                     cGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGcc                                >  1:310455/1‑247 (MQ=255)                                                                                                                                                                                                                                tttgaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATgg                  >  1:116837/4‑250 (MQ=255)                                                                                                                                                                                                                                  tgaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGaa                <  2:124029/249‑1 (MQ=255)                                                                                                                                                                                                                                   gaagagAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGTCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGa                 >  1:434434/1‑248 (MQ=255)                                                                                                                                                                                                                                    aagagAAATGTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGAc             <  2:123910/251‑1 (MQ=255)                                                                                                                                                                                                                                       agaaaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGc          <  2:349473/249‑1 (MQ=255)                                                                                                                                                                                                                                          aaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGAt       >  1:7983/1‑251 (MQ=255)                                                                                                                                                                                                                                             ttGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGc     <  2:118697/250‑1 (MQ=255)                                                                                                                                                                                                                                               gctgcgcAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGcgct  >  1:86738/4‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACT  >  NC_000913/3785541‑3786029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑