Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,730	T→C	T157T (ACT→ACC)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,730	0	T	C	100.0%	207.1 / NA	64	T157T (ACT→ACC)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/34);  total (30/34)

GTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACC  >  NC_000913/3785490‑3785976                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       gtATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCa                                                                                                                                                                                                                                              <  1:395276/251‑1 (MQ=255)   aTCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGt                                                                                                                                                                                                                                            >  2:321877/1‑251 (MQ=255)       ggACCTGCCTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCTCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATTGGGCTGCGCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCTATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTTCCCGCACGCATTCCGTGACGGGCTCGACACCCCGCCGCGGAGTGCTg                                                                                                                                                                                                                                        <  2:432369/251‑1 (MQ=255)       ggACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAAGCCAGTGCTGACTGGCGCTGTAGATAAAGCGAAAAACGCAGGTAAATCAGTACACATTATGGTTCTGCTCGCCGCAGGCGGCGTACACAGCCACGAAGAGCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCATAAAAAATCTACCTGCACGCATTCCTTGACGGGCGCGACACCCCGCTGCGCAGTGCTg                                                                                                                                                                                                                                        <  2:432372/251‑1 (MQ=255)        gACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAACAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTg                                                                                                                                                                                                                                        >  1:101811/1‑250 (MQ=255)            tGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATcc                                                                                                                                                                                                                                   >  1:275578/1‑251 (MQ=255)              acgcGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTc                                                                                                                                                                                                                                 <  2:156849/248‑1 (MQ=255)                 cGTCTGGACGTTGAAATCAAAGATCGCTCTATCTTTGCTAATCCAGTTCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATTGGTCTGCTCTCCGCAGGCGTCGTACACAGCCACGAAGATCACATCATGTCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGc                                                                                                                                                                                                                               <  1:47706/250‑1 (MQ=255)                 cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGc                                                                                                                                                                                                                               <  1:316068/250‑1 (MQ=255)                  gtgtGGACGTTGAAATAAAAGATCGCTCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATGAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTCCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCAGGGCGATGGTAGAACTTGCAGCTGAACGCGGCTCAGAAAAAATCTACCTGCACTCATTCCTGGACGGTCGCGACACCTCGCCGCGCAGTGCTGAATCCTCGCTg                                                                                                                                                                                                                             <  2:376139/248‑1 (MQ=255)                      ggACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGaaa                                                                                                                                                                                                                          >  1:373766/1‑250 (MQ=255)                                aTCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTgaaga                                                                                                                                                                                                               <  1:406125/251‑1 (MQ=255)                                   aaaGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCCGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGAGACACCCCGCCGCGCAGTGCTGAATACTCGCTGAAAAAATTTgaagaga                                                                                                                                                                                                             >  1:391970/1‑248 (MQ=255)                                    aaGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGagaaa                                                                                                                                                                                                           <  2:34558/251‑1 (MQ=255)                                                    tGCTAAACCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAgggt                                                                                                                                                                                            <  2:77194/250‑2 (MQ=255)                                                            cagTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCcg                                                                                                                                                                                    <  2:316434/248‑1 (MQ=255)                                                            cagTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCcg                                                                                                                                                                                    >  1:316434/3‑250 (MQ=255)                                                              gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGAAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGCTGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGACGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAACAATTTGAAGAGAAATTTGCTGCGCAGGGAAAAGGCCGCGt                                                                                                                                                                                 >  2:375823/1‑251 (MQ=255)                                                                  tGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCg                                                                                                                                                                             >  2:122332/1‑251 (MQ=255)                                                                       ggCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTcc                                                                                                                                                                          <  2:117355/249‑1 (MQ=255)                                                                                   aaaGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGtt                                                                                                                                                             >  1:408418/1‑250 (MQ=255)                                                                                     aGCGAAAAACGCAGGGACAACAGTACACATTATAGGTCTGCTATCCGCAGGCGACGTACACAGCCCCGAAGATCACATCATGCCGATGGTAGAACTGGCAGATGAACGCGGCGCAGAAAAAATCTACCTGCACGAATTCATTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCAATCATTGGTCGTtact                                                                                                                                                          >  2:69855/1‑251 (MQ=255)                                                                                            aaCGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGAt                                                                                                                                                   >  2:3917/1‑251 (MQ=255)                                                                                             aCGCAGGTAAAGCAGAACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATTGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCTCCGCGCAGTTCTGAATCCTCGCTGAAAAAATTTGAAGATAAAGTTGCCGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGGTACTACGCGATg                                                                                                                                                  <  2:262234/251‑1 (MQ=255)                                                                                              cGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGATGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATgg                                                                                                                                                 <  1:406285/251‑1 (MQ=255)                                                                                               gCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGACCTGGCAGCTGAACGCGGCGCAGAACAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGAAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATgg                                                                                                                                                 >  1:424434/1‑250 (MQ=255)                                                                                                  ggtaaaGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACcg                                                                                                                                             <  2:329258/248‑1 (MQ=255)                                                                                                     aaaGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACcgcg                                                                                                                                           >  1:349473/1‑250 (MQ=255)                                                                                                             acacATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGtt                                                                                                                                  <  1:222820/251‑1 (MQ=255)                                                                                                                   tATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATc                                                                                                                            >  2:431139/1‑251 (MQ=255)                                                                                                                     tGGGTCTGCTCTACGCAGGCGGCGTACACAGCTACGAAGATTACATCATGGCGCTGGAAGAACTGGCAGCTGAACGCGGCGCAGCAAAAATCTACCTGCACGCATTCCTTGATGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAACAATTTGAAGAGAAATTTGCTGCGCAGGGAAAAGGCCGAGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGAGATAACCGTTGGGATcgc                                                                                                                          >  1:101398/1‑251 (MQ=255)                                                                                                                          cTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCCGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTACAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGttg                                                                                                                      >  1:58121/1‑248 (MQ=255)                                                                                                                            gCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGttg                                                                                                                      <  1:109075/248‑3 (MQ=255)                                                                                                                            gCTCCCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACGGGCAGCTGAACGCGGCGCAGAAAAAAGCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGGTGGGATCGCGttg                                                                                                                      <  2:101811/248‑3 (MQ=255)                                                                                                                                 ccGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGc                                                                                                               <  1:3917/250‑1 (MQ=255)                                                                                                                                            gTACACAGCCACGAAGAACACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATGTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACctgct                                                                                                   <  2:408418/251‑1 (MQ=255)                                                                                                                                              acacAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGaaaaa                                                                                                                 <  2:87057/235‑1 (MQ=255)                                                                                                                                              acacAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGaaaaa                                                                                                                 >  1:87057/1‑235 (MQ=255)                                                                                                                                               cacaGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGAc                                                                                                >  2:402336/1‑251 (MQ=255)                                                                                                                                                    ccACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTgg                                                                                           >  1:336798/1‑251 (MQ=255)                                                                                                                                                      aCGAAGATCACATCAGGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGgcg                                                                                         <  2:338789/251‑1 (MQ=255)                                                                                                                                                         aaGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGgcgc                                                                                        >  1:118697/1‑249 (MQ=255)                                                                                                                                                         aaGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGgcgc                                                                                        >  1:124029/1‑249 (MQ=255)                                                                                                                                                             tCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAgggtg                                                                                  >  2:209280/1‑249 (MQ=255)                                                                                                                                                                   catGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCTCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTc                                                                             <  1:64981/250‑1 (MQ=255)                                                                                                                                                                                  aCTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCAGTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATActgccg                                                             <  1:359389/251‑1 (MQ=255)                                                                                                                                                                                     ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGCTACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCAGTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTg                                                          <  1:205320/251‑1 (MQ=255)                                                                                                                                                                                     ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGtt                                                           <  1:139444/250‑1 (MQ=255)                                                                                                                                                                                     ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTAGACGGACGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAACGGCCGCGTAGCATCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGAAGGGTGAGTTCCAGGCCGCTACTGCCGTTg                                                          >  1:253967/1‑251 (MQ=255)                                                                                                                                                                                            gAACGCGGCGCAGAAAAAATCTACCTGCACGCATCCCTTGACGGTCGCGACAACCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACTCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGTGTGAGTTCCAGGCCGATACTGCCGTTGCTGGttt                                                   <  1:107959/251‑1 (MQ=255)                                                                                                                                                                                             aaCGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTg                                                  >  1:67226/1‑251 (MQ=255)                                                                                                                                                                                                       aGAAAAAAGCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTGCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCtt                                        <  2:278536/251‑1 (MQ=255)                                                                                                                                                                                                        gAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGACCAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACAGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCtt                                        >  2:66010/1‑250 (MQ=255)                                                                                                                                                                                                         aaaaaaTCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTAGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTAt                                      >  2:95167/1‑251 (MQ=255)                                                                                                                                                                                                           aaaaTCTACCTGCACGCATTCATTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGAGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGAAGGCAGCTTATGc                                    >  2:383157/1‑251 (MQ=255)                                                                                                                                                                                                              aTCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAcc                                                                                                                                     <  2:358843/151‑1 (MQ=255)                                                                                                                                                                                                              aTCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAcc                                                                                                                                     >  1:358843/1‑151 (MQ=255)                                                                                                                                                                                                                  aCCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTcgct                               <  2:275578/249‑2 (MQ=255)                                                                                                                                                                                                                       cACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGaaa                         >  2:130203/1‑250 (MQ=255)                                                                                                                                                                                                                           cATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCGCGCGACGaaaacgac                    <  1:122332/251‑5 (MQ=255)                                                                                                                                                                                                                           cATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGACCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGaaaacgac                    <  1:321877/251‑5 (MQ=255)                                                                                                                                                                                                                            aTTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGACATTTGCTGCGCAGGGAAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGaaaacgacg                   >  1:297514/1‑246 (MQ=255)                                                                                                                                                                                                                                       gTCGCGACACCCCTCCGCGCTGTGCTGAATCCTCGCTGAAAAAATTTGAAGATAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTTAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTTAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGaat        <  2:208729/251‑2 (MQ=255)                                                                                                                                                                                                                                             acacCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGAcc  <  2:122191/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       GTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACC  >  NC_000913/3785490‑3785976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑