Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,397	A→G	L46L (CTA→CTG)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,397	0	A	G	100.0%	166.0 / NA	47	L46L (CTA→CTG)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/19);  total (28/19)

GCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCG  >  NC_000913/3785160‑3785626                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      gCCGCGAGCGGTCGTATAGTTTGATCGTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTcc                                                                                                                                                                                                                           <  2:10991/250‑1 (MQ=255)      gAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGCTTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTg                                                                                                                                                                                                                     >  2:306634/1‑251 (MQ=255)       aGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCCACTTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGGGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGACATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTgg                                                                                                                                                                                                                    >  2:107959/1‑251 (MQ=255)        gCGGTCGTATATTTTGAGCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGa                                                                                                                                                                                                                   <  2:344064/251‑1 (MQ=255)             cGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTc                                                                                                                                                                                                               <  1:50681/250‑1 (MQ=255)              gaatatTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCgg                                                                                                                                                                                                             >  2:109075/3‑251 (MQ=255)                atatTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGAACTGGTGATTCTGGATGGCTATGGCTATCGTGAGGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTACTGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGCCGGTc                                                                                                                                                                                                           >  1:156849/1‑251 (MQ=255)                             cAGATGCTATTTTAGCGATTCAGCTGTAGTAAAATTACGCAAATGTTGACTCTTGAGTATGATGGTGTCGCAATGTCGGTTGCTAAAAAACCGATGGTTCTGGTGATTCTGGTTGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTGTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGTCCAATCCGCCGCATACCCTGATCGACCCGTCCTGTCTGGAAGTCTGGCTGGCGGACCGTCa                                                                                                                                                                                              <  2:411176/251‑1 (MQ=255)                             cAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGAAATGGATGCACTGTGGGCCAATCGTCCGCATACACTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTc                                                                                                                                                                                               >  1:203893/1‑250 (MQ=255)                                  gCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTATAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCCATCGTCCGCATACCCTGATCGACGATTCAGGTCTGGAAGTCGGTCTGCCTGACCGGCAGATgg                                                                                                                                                                                         >  1:438265/1‑251 (MQ=255)                                    tATTTTATCGCTTCAGCTGTAGTAAAATTACGCAACTTTTGACTCTTGCGTCTGAGGTTGTCGCAATGTCGGTTGCTAAAAAACCTATGGTACTGGGGATTCTGGTTGGCTATGGCTATCGTGAAGCACAGAAGGATCACGCCATTTTTCGAGCTAAAACCCCGGTAATGGATGCACTGTGGCCCAATCGTCCGCATACCCTGATCGACGCTTCCGGACAGGAAGACGGTCTGCCTGTCCGTCAGATGGGt                                                                                                                                                                                       >  1:423847/1‑251 (MQ=255)                                       tttATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAAc                                                                                                                                                                                    >  1:77194/1‑251 (MQ=255)                                                                          ttGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGcc                                                                                                                                                                       >  1:389734/1‑229 (MQ=255)                                                                          ttGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCGATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAGCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCGGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGcc                                                                                                                                                                       <  2:389734/229‑1 (MQ=255)                                                                               tctTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCg                                                                                                                                            >  2:337192/1‑251 (MQ=255)                                                                               tctTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCg                                                                                                                                            >  1:255202/1‑251 (MQ=255)                                                                                            gTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc                                                                                                                                >  1:346510/1‑250 (MQ=255)                                                                                            gTTGTCGCAATGTCGGTGTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc                                                                                                                                <  2:346510/250‑1 (MQ=255)                                                                                              tGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGACGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGGGCCGGCCGCCTCGTGGATCAGGACCTg                                                                                                                              >  2:268832/1‑250 (MQ=255)                                                                                                                      aCCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGACGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAg                                                                                                     <  2:57990/251‑1 (MQ=255)                                                                                                                         tagggTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTGCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCAGCGTGTa                                                                                                                                        <  2:306673/210‑1 (MQ=255)                                                                                                                         tATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTATGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTa                                                                                                                                        >  1:306673/1‑213 (MQ=255)                                                                                                                          aTGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATc                                                                                                  <  2:213765/250‑1 (MQ=255)                                                                                                                               actggttgttcgtgATGGCTATGGCTATCGTTAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATAGTCCGCATGCCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTATGCCACGTTAACCTGGGTGCCTGCCGCATCTTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctt                                                                                            <  2:421521/238‑1 (MQ=255)                                                                                                                                     gATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCTCTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGgtttcttt                                                                                       <  2:355337/250‑1 (MQ=255)                                                                                                                                              tggctatggctaTCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATcc                                                                               >  1:34558/1‑249 (MQ=255)                                                                                                                                               ggctatggctatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGGCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGGTAACCTGGGTGCCGGCCGCATCGTGTATCAGTACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCGAATccag                                                                             <  2:295648/250‑3 (MQ=255)                                                                                                                                                   atggctatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGc                                                                          <  1:394352/249‑1 (MQ=255)                                                                                                                                                         atCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGgc                                                                  >  1:208729/1‑251 (MQ=255)                                                                                                                                                                agaaCAGCCGGATAACGCCATTTTTAGTACTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGACATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGCCTGGCGCGGt                                                             >  2:359389/1‑249 (MQ=255)                                                                                                                                                                  aaCAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGt                                                             >  1:426328/1‑247 (MQ=255)                                                                                                                                                                              aCGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAAc                                             <  1:337192/251‑1 (MQ=255)                                                                                                                                                                                 ccATTTTTAGTGCTCAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGc                                           >  1:117355/1‑250 (MQ=255)                                                                                                                                                                                       ttAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGGCGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCGGACTGGCTCGGTAGATAAAGCGAAAAACGCAggtaa                                     <  2:255202/250‑4 (MQ=255)                                                                                                                                                                                         aGTGCTAAAAACCCAGTAAAGTATGCACTATGGGACAATCGTCCGCATACCCTGCTCACCGCTTCAAGTATGGAAGTAAGTCTGCCTGAAAGTCAGATGGGAAACTAAGAAATCGACCAAGATAACATGGGTGCCGGAAGCATCGTGTATCAGGAACTATCTCGTCTGGACGTTGCAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAACGAAAAACGCAggtaa                                     >  2:182789/1‑245 (MQ=255)                                                                                                                                                                                          gTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGc                                  >  2:316068/1‑250 (MQ=255)                                                                                                                                                                                                  aCCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGTAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCCGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATCAAACGAAAAACGCAGGTAAAGCATTACACAt                         >  1:128666/1‑251 (MQ=255)                                                                                                                                                                                                   ccgcGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGCCGCTTCCGGTATGGAAGTCGGTCTGCCTGACCGTCAGATTGTTAAATCCGCAGTAGGAAACGTTAACCTGGGCGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGCCGTTGAAGTCAATGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAGGCGAAATACGccagtaaatcagtacacgtt                        >  2:233891/4‑232 (MQ=255)                                                                                                                                                                                                        gTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGTAATTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATggg                   <  1:289656/251‑1 (MQ=255)                                                                                                                                                                                                         tAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGt                  >  2:205320/1‑251 (MQ=255)                                                                                                                                                                                                           aTGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTCACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCt                >  2:139444/1‑251 (MQ=255)                                                                                                                                                                                                           aTGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATcca                                                                              >  2:2963/1‑188 (MQ=255)                                                                                                                                                                                                           aTGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATcca                                                                              <  1:2963/189‑2 (MQ=255)                                                                                                                                                                                                            tGGATGCACTGTGGGCCACTCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCCTCGTGTATCAGGACCTGACTCGTCTGGACGTTGAACTCAAAGATCGCGCTTTCTTTGCTAATCTAGTGCTGACTGGCGCGGTAGATAAAGCGAACAACGCAGGTAAAGCGGTACACATTATGGGTCTg               >  2:58041/1‑251 (MQ=255)                                                                                                                                                                                                             ggATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTg               <  1:132069/250‑1 (MQ=255)                                                                                                                                                                                                             ggATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGc              >  2:406285/1‑251 (MQ=255)                                                                                                                                                                                                                         ggCCAATCGTCCGCATGCCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAggcg  <  1:184790/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      GCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCG  >  NC_000913/3785160‑3785626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑