Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,061,935	G→A	S46N (AGC→AAC)	torD →	trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,061,935	0	G	A	93.6%	130.3 / NA	47	S46N (AGC→AAC)	torD	trimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/19);  minor base C (0/3);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGAT  >  NC_000913/1061694‑1062181                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ggAAATTGAGAGGTACAACGGAACAGTGGAGCAGGTGCCGTGGTTAAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGAGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCGCCCTTGAGCTGGACGATGAACAACTGACGCAAATCGCCATTGCGCAGATGCCTGAATGGTTTTCGT‑TGCTTAAAAACGAAccgcc                                                                                                                                                                                                                                               <  2:283372/251‑1 (MQ=255)    aaTTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCt                                                                                                                                                                                                                                            >  1:202060/1‑251 (MQ=255)                    ggAACAGTGGAGCAGGTGACGGCTTTTAACGCCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGCCCCCGCTGACAGCACAACATATTGCCTGTTTTTCCGCCTGGCTAGCGCAGTTTTTCTCCCTTGAGCTGGCCGATGAACACCTGACGCAAATCGCCAGTGCGCAGATGGCGGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAAc                                                                                                                                                                                                                            <  1:21100/251‑1 (MQ=255)                            ggAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCAAAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGCGCTggg                                                                                                                                                                                                                    >  2:100878/1‑250 (MQ=255)                                     gACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAATGAACCGCCGCTCACTGCGGCGGTGAACGGGCTGGAAAACCGTAt                                                                                                                                                                                                           >  1:284004/1‑251 (MQ=255)                                                                  gggCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCGCGCTGACAGCAAAACAGATTGCCTGTGTTTACGCCTGCCTAGCGCAGTTTTTCCCCCGTGAGCTGGACGATGAACAACTGCCGAAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGccc                                                                                                                                                                              <  1:376866/250‑1 (MQ=255)                                                                                      cccGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGAc                                                                                                                                                          >  1:315458/1‑251 (MQ=255)                                                                                          cgTCGCAGGTGAAAGCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACtttt                                                                                                                                                      <  1:140236/251‑1 (MQ=255)                                                                                             ggaaGGTGAAAGCATGTCCCCGCTGACAGCACAACAGATTGGTTGTGTTTACGCTGGGCTAGGGCAGTTGTTCTCCCGTGGGCTGGACGATGAACAACGGACGCAAACCGCAAGTGCGCAGATGGTGGAAGGGTTTTCGT‑TGCTAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGc                                                                                                                                                    <  1:433941/247‑1 (MQ=255)                                                                                             ggCAGGTAAAATCATCACCGCGCCGACAGAACAACAGTTTGCCTGTGTTTACGCCGGGCTGGCGCAGTTGTTCGCCGGTGAGGGGGACGAGGAACAAGTGACGAAAAGCGCCAGGGCGCAGGTGGCTGAAGTGTGTTGGT‑TGCAGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCGGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGTCTTTTGCg                                                                                                                                                   <  2:214640/250‑1 (MQ=255)                                                                                             cgaaggtaaaacaatgcccACGCTGACAGCACAAAAGATTGCCTGTTTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCGGGACGATAAACAACTGACGAAAATCGCCAGTGCGCAGATGGCTGAAGGGTTTTCGT‑TGTTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCg                                                                                                                                                   <  1:374440/234‑1 (MQ=255)                                                                                              gCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCgg                                                                                                                                                  >  1:83073/1‑251 (MQ=255)                                                                                                aGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGAGGACGAGCTGGAAAACCGGATTGCCACGCTGCCAGTGCGTGGCGATTCCCCTCTGGAACACGTCGCGGACCTTTGCGGcc                                                                                                                                                >  2:388422/1‑251 (MQ=255)                                                                                                 agTGACATCATGCCCACGCTGACAGAACAACAGCTTGCCTGTGTTTACGCCTGGATAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACCACTGACGCAACTCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGCAAAGCCGCTCCCTGCGGCGGGGAACGAGTCGGAAACACGAATTGCCACGCTGACGGGTCGAGACACTGCCCGTCAGGCCCTGGCCGCGGGCTTTTGAGGCCt                                                                                                                                               >  2:387232/2‑251 (MQ=255)                                                                                                  gtaaaaccaggACCCCGCGGACACCACAACAGATTGCCTGTGTTTCCCCTTGGCTGGCGCATTGGTGCTCCCGGGGGCTGGACGATGACAAACGGACGCAAATCGCCAGTGCGCAGATGGGTGAATGGTTGTGGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTg                                                                                                                                              <  2:79051/241‑1 (MQ=255)                                                                                                   tGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGt                                                                                                                                             >  1:70231/1‑251 (MQ=255)                                                                                                            gACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGCATTGCCACGCTGACAGTACGGGACGATGCCCGTCTGGAACTTGCCGCGGGCTTTTGCGGCCCGTTTCtgatg                                                                                                                                     >  2:183786/1‑250 (MQ=255)                                                                                                                     gACAGCACAACAGATTGCATGAGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACCAGCTGGAAAAACGTATTGCCACGCTGACAGTACGGGACGGTGCCCGTCTGGAACAGGCCGCGGACTTTTGCGGCCTGTTTTTGATGAAAGacaaa                                                                                                                            >  2:104889/1‑250 (MQ=255)                                                                                                                                  attgctggttttgaCGCATGGCTAGCGAAGTTGTGCGCCGGTGAGCGGGACGAGGAAAAACGGACGCAAACCGCCAGTGCGAAGAGGGCTGAAGGGTTTTCGT‑GGCGAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGcc                                                                                                              <  2:69045/238‑1 (MQ=255)                                                                                                                                  aTTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTTGCCGCGGACTTTTGCTGCCTGTTTCTGCAGACCGACCAACAAGCGGCgccgcc                                                                                                              >  2:8276/1‑251 (MQ=255)                                                                                                                                     gCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGACCGTCTGGAACTGGCCGCGGCCTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGt                                                                                                            >  1:413716/1‑250 (MQ=255)                                                                                                                                      ccTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAACTCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGGTTCTGGTGACCGTCAAAAAAGCGGGGCGGCCGTAt                                                                                                          >  2:132157/1‑251 (MQ=255)                                                                                                                                        tgtgTTTCCGCCTGGCTAGCGCAGTTGTTCCCCCGTGAGCTGGACGATAAACAACTGACGAAAATCGCCAGTGCGCAGAGGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATg                                                                                                         <  1:59261/250‑1 (MQ=255)                                                                                                                                               aCGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAAAAAGCGGCGCTGCCGTATGCCTCGGcc                                                                                                 >  1:11036/1‑251 (MQ=255)                                                                                                                                                         aGCGCAGTTGTTCCCCCGTGGGCGGGACGATGAAAAACGGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAgg                                                                                       <  1:243491/251‑1 (MQ=255)                                                                                                                                                              gtggtttctccggtgtgggggccGATGAACAACTGACCCACTTCGCCTGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAACCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAg                                                                                  <  2:6177/229‑1 (MQ=255)                                                                                                                                                                            gAGCTGGACGATGCACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAAACGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCCAGAGATTAAAcc                                                                    >  2:81247/1‑250 (MQ=255)                                                                                                                                                                                   aCGATGAACAACTGACGAAAATCGCCAGGGCGCAGAGGGCTGAATGGTTTTCGT‑TGCTGAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTa                                                             <  1:375368/251‑1 (MQ=255)                                                                                                                                                                                     gATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGAATTGCCACGCTGCCAGTACGTGACGATGCCCGGCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATCACCGACAAACACGCCGCGCTGCCGTATTGATCGGCCTCCCAACAGGACGCGCAAGAGATCAAACGGTTGTTAGt                                                           >  2:348966/1‑251 (MQ=255)                                                                                                                                                                                          acaacTGCCGCAAATCGCCCGCGCGCAGATGGCTGTCTGGTTTTCGT‑TGCTGCAAAACGAACCGCCGCTCACTGCGGCGGTGAATGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATTCACGTCTGGTAATGGCCGCGGACTTTTGAGGCCTGTTTCTGATGACCGTCAAAAAAGCGGCGCTGCCGGATGCATCGGCCTACAAACAGGACGAGCCAGAGATTAAACGATTGATAGTTTAgg                                                      >  2:220532/1‑251 (MQ=255)                                                                                                                                                                                             acTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAg                                                   <  1:354432/251‑1 (MQ=255)                                                                                                                                                                                             acTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAACGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGc                                                     >  2:349423/1‑249 (MQ=255)                                                                                                                                                                                                gACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTTCGGCCTGTTTCtgatg                                                                                                                                     >  1:55992/1‑166 (MQ=255)                                                                                                                                                                                                   gCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGGTgg                                             >  1:327295/1‑251 (MQ=255)                                                                                                                                                                                                      aaccGCCAGGGCGCAGATGGCTGAGTGGTTTTCGT‑GGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATAGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGaaa                                          <  1:186110/248‑1 (MQ=255)                                                                                                                                                                                                      aaTCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGaa                                           >  1:376177/1‑250 (MQ=255)                                                                                                                                                                                                             gttGCGCAGATGGCAGAATGGTTTTCGT‑GGCT‑AAAACCGACCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGc                                  <  1:267680/249‑1 (MQ=255)                                                                                                                                                                                                                gcgcAGTTGTCTGAATGGTTTT‑GTGTGATGAAAAACAAACCGCCGCGCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTTGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGGATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCaa                                <  1:429984/251‑1 (MQ=255)                                                                                                                                                                                                                  gcAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTAACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACCGTACGGGACGATGCCCGTCTGGAACTTGCCGCGGACTTTTGCGGCCTGTTTCTGCTGACCGACAAACCAGCGGCGCTGACGTATGCATCGGCCTACAAACAGGACGAGAAAGAGATTAAACGCTTGTTATTTGAGGCAGGGATGGAAACCCGCGGCactt                              >  2:309388/1‑248 (MQ=255)                                                                                                                                                                                                                     gATGGCGGAATGTTTTTCGT‑TGCTAAAAAACGACCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCa                           <  2:315458/251‑1 (MQ=255)                                                                                                                                                                                                                        ggCTGAATGGTTTTCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACCGTACGTGACGATGCCCGTCTGGAAATGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGCGATTAACCGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGAAATTTCAACg                        >  2:369629/1‑251 (MQ=255)                                                                                                                                                                                                                            aaaTGGTTTTCGT‑TGCTAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAAc                     <  1:183786/249‑1 (MQ=255)                                                                                                                                                                                                                              agggTTTTCGT‑TGCTAAAAACCAACCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCgg                  <  2:202060/249‑1 (MQ=255)                                                                                                                                                                                                                                   tttCGT‑TGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGc                 >  1:325553/1‑247 (MQ=255)                                                                                                                                                                                                                                    ttCGT‑TGCTAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGat             <  1:349423/250‑1 (MQ=255)                                                                                                                                                                                                                                           ggtaaaaaACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATTCACGTCTGGAACTGGCCGCGGTCTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGATGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTgg      <  2:242742/247‑1 (MQ=255)                                                                                                                                                                                                                                               aaaaACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAAACGGCAGATCATCTGGCGat  >  1:130467/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGT‑TGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGAT  >  NC_000913/1061694‑1062181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑