Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,264	T→C	L2S (TTG→TCG)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,264	0	T	C	100.0%	120.4 / NA	40	L2S (TTG→TCG)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/17);  total (23/17)

AACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAAAAAAAAGCGTAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTG  >  NC_000913/3785023‑3785502                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               aacaacCCCCGACGTAGAGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCAAGCGCGTTAACTGGAATGCACTTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGGCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTAAGCAAATTTTGACTCTTGAGTGTGAGGTTGTCTCAATGTCGGtttata                                                                                                                                                                                                                                        >  2:395276/1‑247 (MQ=255)                       aaaaaaaCCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGAtt                                                                                                                                                                                                                 <  1:378623/251‑1 (MQ=255)                         aaaaaCCAGGAAAGGAGTAGACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTGTGATCGTCAGAGGCTATGTTATCGATTCAGCTGTAGTAAAAGTACGCAAATGTTGACTCTTGAGTATTATGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCt                                                                                                                                                                                                               <  1:398151/251‑1 (MQ=255)                          aaaaCCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGCCGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCCGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTCTGAGGTTTTCGCAATGTCGGTTTATAAAAAACCTATGGGACTGGGGATTCTg                                                                                                                                                                                                              >  1:411176/1‑251 (MQ=255)                            aaCCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTTGTGATTCTGGa                                                                                                                                                                                                            <  2:266328/251‑1 (MQ=255)                                 ggAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGGGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGCAGTAAAAGTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAAGGTCGGTTTCTAAAAAACCTATGGTACTTGTGATTCTGGatggct                                                                                                                                                                                                       <  2:55908/251‑1 (MQ=255)                                    aaGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggctatg                                                                                                                                                                                                    <  2:240075/251‑1 (MQ=255)                                               cTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCCTCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGGACTGGTGATTCTGGATGGCTATGGCTATcgtga                                                                                                                                                                                          >  1:295648/1‑247 (MQ=255)                                                tGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATcgtga                                                                                                                                                                                          >  1:344064/1‑246 (MQ=255)                                                         cGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAgg                                                                                                                                                                               >  1:121398/1‑251 (MQ=255)                                                                 acatccagcGCGGTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGTATAACGc                                                                                                                                                                        <  2:270617/246‑1 (MQ=255)                                                                      cagcGCGTTAACTGGAATGCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCAtttt                                                                                                                                                                  <  2:329177/251‑1 (MQ=255)                                                                        gcgcGGTAACTTGAATGCAATTGTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCTGTCGTATATTTTGATCTGCAGAGGCTATTTTATCTATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCGTGAGTATTAGGTTGGCGCAATGCCGGGTTCTAAAAAACTTGTGTCACTGGGGTTTCTGTATGGCTATGGCTAGCTTGAAGTACAGCAGTATAGCGCCATTTTTa                                                                                                                                                                <  2:428853/251‑1 (MQ=255)                                                                                        gCAATTTTGCGGGGCACGACGAAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCggg                                                                                                                                                >  1:213765/1‑250 (MQ=255)                                                                                                      cacgacgaAAAAAAAGCGCAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACtgt                                                                                                                                  <  1:154843/249‑1 (MQ=255)                                                                                                                      cgcaaaTCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGc                                                                                                                   >  1:338789/4‑250 (MQ=255)                                                                                                                       gcaaaTCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGTTCTTCAGAGGCTATTTTATCGATTCCACTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGATCTGGTGATTCTGGacggctatg                                                                                                                                                                                                    >  2:130377/3‑168 (MQ=255)                                                                                                                       gcaaaTCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGGCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATGCTGGatggctatg                                                                                                                                                                                                    <  1:130377/166‑1 (MQ=255)                                                                                                                       gcaaaTCAGGCATCGGCCTGCCGCGAGCGGTCGTATATTTTGATCCTCAGAGGCTATTTTAACCATTAAGCTGTAGTAAAATTACGCCAATTTTGACTCTTGAGTATGAGGTTGTAGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAt                                                                                                                 >  2:222820/3‑251 (MQ=255)                                                                                                                                          gCCGCGAGCGGTCGTATAGTTTGATCGTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTcc                                                                                               <  2:10991/250‑1 (MQ=255)                                                                                                                                               gAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGCTTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTg                                                                                         >  2:306634/1‑251 (MQ=255)                                                                                                                                                aGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCCACTTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGGGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGACATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTgg                                                                                        >  2:107959/1‑251 (MQ=255)                                                                                                                                                 gCGGTCGTATATTTTGAGCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGa                                                                                       <  2:344064/251‑1 (MQ=255)                                                                                                                                                      cGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTc                                                                                   <  1:50681/250‑1 (MQ=255)                                                                                                                                                       gaatatTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCgg                                                                                 >  2:109075/3‑251 (MQ=255)                                                                                                                                                         atatTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGAACTGGTGATTCTGGATGGCTATGGCTATCGTGAGGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTACTGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGCCGGTc                                                                               >  1:156849/1‑251 (MQ=255)                                                                                                                                                                      cAGATGCTATTTTAGCGATTCAGCTGTAGTAAAATTACGCAAATGTTGACTCTTGAGTATGATGGTGTCGCAATGTCGGTTGCTAAAAAACCGATGGTTCTGGTGATTCTGGTTGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTGTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGTCCAATCCGCCGCATACCCTGATCGACCCGTCCTGTCTGGAAGTCTGGCTGGCGGACCGTCa                                                                  <  2:411176/251‑1 (MQ=255)                                                                                                                                                                      cAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGAAATGGATGCACTGTGGGCCAATCGTCCGCATACACTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTc                                                                   >  1:203893/1‑250 (MQ=255)                                                                                                                                                                           gCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTATAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCCATCGTCCGCATACCCTGATCGACGATTCAGGTCTGGAAGTCGGTCTGCCTGACCGGCAGATgg                                                             >  1:438265/1‑251 (MQ=255)                                                                                                                                                                             tATTTTATCGCTTCAGCTGTAGTAAAATTACGCAACTTTTGACTCTTGCGTCTGAGGTTGTCGCAATGTCGGTTGCTAAAAAACCTATGGTACTGGGGATTCTGGTTGGCTATGGCTATCGTGAAGCACAGAAGGATCACGCCATTTTTCGAGCTAAAACCCCGGTAATGGATGCACTGTGGCCCAATCGTCCGCATACCCTGATCGACGCTTCCGGACAGGAAGACGGTCTGCCTGTCCGTCAGATGGGt                                                           >  1:423847/1‑251 (MQ=255)                                                                                                                                                                                tttATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAAc                                                        >  1:77194/1‑251 (MQ=255)                                                                                                                                                                                                       aTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggct                                                                                                                                                                                                       >  1:280714/1‑85 (MQ=255)                                                                                                                                                                                                       aTTACGCAAATTTTGACTCTTGAGTAGGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggct                                                                                                                                                                                                       <  2:280714/85‑1 (MQ=255)                                                                                                                                                                                                                   ttGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGcc                                           >  1:389734/1‑229 (MQ=255)                                                                                                                                                                                                                   ttGACTCTTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCGATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAGCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCGGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGcc                                           <  2:389734/229‑1 (MQ=255)                                                                                                                                                                                                                        tctTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCg                >  2:337192/1‑251 (MQ=255)                                                                                                                                                                                                                        tctTGAGTATGAGGTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCg                >  1:255202/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTTGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc    >  1:346510/1‑250 (MQ=255)                                                                                                                                                                                                                                     gTTGTCGCAATGTCGGTGTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc    <  2:346510/250‑1 (MQ=255)                                                                                                                                                                                                                                       tGTCGCAATGTCGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGACGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGGGCCGGCCGCCTCGTGGATCAGGACCTg  >  2:268832/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               AACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAAAAAAAAGCGTAAATCTGGCATCGGCTTGCCGCGAGCGGTCGTATATTTTGATCTTCAGAGGCTATTTTATCGATTCAGCTGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTG  >  NC_000913/3785023‑3785502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑