Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,474,424	T→G	K43N (AAA→AAC)	rpsL ←	30S ribosomal subunit protein S12

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,474,424	0	T	G	100.0%	192.5 / NA	55	K43N (AAA→AAC)	rpsL	30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/27);  total (28/27)

ATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAA  >  NC_000913/3474177‑3474646                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               aTTAAGCCTTAGGACGCTGCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTa                                                                                                                                                                                                                             <  1:302517/251‑1 (MQ=255)   tAAGCCTTAGGACGCTTCCCGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAg                                                                                                                                                                                                                            <  2:165243/250‑1 (MQ=255)   tAAGCCTTAGGACGCTTCACGCAATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAg                                                                                                                                                                                                                            <  2:26513/250‑1 (MQ=255)       ccTTATGACGCTTGAGGCCTTACTTGCCACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCAGGGCGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGAGCTCCTGCAGGGTGGGAACTTCACCACCGATGAAGGAAATCAATTCGAAACCGTTAGTCAAACGAACACGGCATACTTTACGCAACGCGGAGTTCGGGTTGTTAGGAGTg                                                                                                                                                                                                                       >  2:263604/1‑251 (MQ=255)       ccTTAGGACGCTTCCCGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTg                                                                                                                                                                                                                       <  1:110521/251‑1 (MQ=255)        cTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTgg                                                                                                                                                                                                                      >  1:45115/1‑251 (MQ=255)           aGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGgt                                                                                                                                                                                                                     >  1:216049/1‑249 (MQ=255)                           tACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAAAACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGAATATACACGAGTAc                                                                                                                                                                                                    >  1:392853/1‑250 (MQ=255)                                           gtttACGGTTTTAAACGCCGGAGCAGTCAAGCGCACCACTTACGGTGTGGAAACGAACGCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGc                                                                                                                                                                                   <  2:329308/249‑1 (MQ=255)                                            cTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCg                                                                                                                                                                                  >  2:273949/1‑251 (MQ=255)                                                 ggTCTTTACCGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCAt                                                                                                                                                                             >  2:299849/1‑251 (MQ=255)                                                       tAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGTTAACGAACCCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTc                                                                                                                                                                        <  2:16439/250‑1 (MQ=255)                                                        aaCGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGCGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTcc                                                                                                                                                                       >  1:22537/1‑250 (MQ=255)                                                          cGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAgc                                                                                                                                                                    >  1:332344/1‑251 (MQ=255)                                                             cGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGCCCTTCACCACCGATGTCGGACGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAgcgc                                                                                                                                                                  >  1:256693/1‑250 (MQ=255)                                                              ggAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCAAGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAgcgc                                                                                                                                                                  >  2:396980/1‑249 (MQ=255)                                                                ggcaggctagagctactcGTACGGTGTGTTGCCGAACACCCGGGTGTTCTTTAACACGTCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGGTGTGACCTGCTCGACCGAGGTGGGAAGTCACTTCGAATGCTTTAGTCAGACGAACACGTCATACTTTACGCATCGCTGAGTTCGGTTTGTTAGGGGTTTTATTACATACACGAGTACATACTTCTGGTTGTTGGGGTCATGCTTCCAGCGCatgc                                                                                                                                                              <  2:207206/234‑4 (MQ=255)                                                                          cgcACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCttt                                                                                                                                                    >  1:137973/1‑251 (MQ=255)                                                                             accacGTCCGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGCCCGCCACGGATCAGGATCACGGCGTGCTCCTGCCGGTTGTGACCTTCCCCACCGCTGTAGGAAGTCACTTCGAAACCCTTCGTCCGACGCCCACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGCGTGGTCGTATATACACGAGTACATACGCCACGTTTTTGCGTGCATGCTTCCAGCGCAGGCACGTTGCTTTTCg                                                                                                                                                 >  2:192225/1‑251 (MQ=255)                                                                                     cGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATAAGGATCACGGAGTGATCCTGCAGGCTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAAAACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGACACGTTGCTTTTCGCAACTTTg                                                                                                                                         >  2:409372/1‑251 (MQ=255)                                                                                            gTAACGAACACCCGGGCGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGCGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCAc                                                                                                                                  >  2:96083/1‑251 (MQ=255)                                                                                              aaCGAACCCCCGGGAGGTCTTTAACCCGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGt                                                                                                                                <  1:240121/251‑1 (MQ=255)                                                                                                    cccccGGGAGGTCTTAAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTg                                                                                                                          <  2:94708/249‑1 (MQ=255)                                                                                                        cGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTa                                                                                                                      <  1:282783/251‑1 (MQ=255)                                                                                                           gAGGGCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCa                                                                                                                   <  2:37485/251‑1 (MQ=255)                                                                                                               tCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTACGTACCAGCTg                                                                                                               >  1:103569/1‑251 (MQ=255)                                                                                                                cTTTAACACGACCGCCACGGATCAGGATCAAGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTgg                                                                                                              >  1:84544/1‑251 (MQ=255)                                                                                                                 tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCAGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGt                                                                                                             >  1:2021/1‑251 (MQ=255)                                                                                                                  ttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTg                                                                                                               <  1:227616/248‑1 (MQ=255)                                                                                                                                       aggatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCt                                                                                       <  2:216049/251‑1 (MQ=255)                                                                                                                                         gatcaCGGGGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGGCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTcc                                                                                     <  2:96562/251‑1 (MQ=255)                                                                                                                                           ccaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCAGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCtt                                                                                   <  2:2021/250‑2 (MQ=255)                                                                                                                                              cGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTATGAAGTCACTTAGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGGGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtt                                                                                >  2:242973/1‑251 (MQ=255)                                                                                                                                                  gTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtttt                                                                              >  1:56459/1‑249 (MQ=255)                                                                                                                                                          gCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTg                                                                      <  2:331612/249‑1 (MQ=255)                                                                                                                                                            gggTTGTGACCTGCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGTTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGGTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTc                                                                  <  2:137973/250‑1 (MQ=255)                                                                                                                                                            agggtgggACCTTCACCACCGATGTAGGAAGTCACTTCGAACCCGTTAGTCAGACGAGCACGGCATACTGTTCGCAGGGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACCCGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTGGTTTGTCGCAACTTTCAGAGCACGTGGGTTGCGTTCCAGCTGGTTATCTGTTGCTATTAAATAGCTCCTTGTTTTAGCTGTTGCTTc                                                                  <  2:431283/244‑1 (MQ=255)                                                                                                                                                               ttGTGACCTTCACCACCGATGTAGGAAGCCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGt                                                                >  1:328336/1‑250 (MQ=255)                                                                                                                                                                 gtgACCTTCACTACCGATGTTGGAAGTCACTGCGAACCCGTTAGTCAGACGAACACGGCATTCTTTGCGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCCCGTTTATGCGGGCATGCTTCCAGCGCAGGCACGCTGTTTTTCGCAACTTTGCGAGCCCGAGGTTTGCGTACCAGCTGGTTAACGGTGGCCATTACATATCTCCTGGTGTTAGCGTTTGCTTCGTaaa                                                             <  2:100011/251‑1 (MQ=255)                                                                                                                                                                    aCCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAacac                                                          >  1:65741/1‑251 (MQ=255)                                                                                                                                                                           ccaccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaata                                                    >  2:346925/1‑250 (MQ=255)                                                                                                                                                                                     aGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTca                                         <  1:396980/251‑1 (MQ=255)                                                                                                                                                                                        aaGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCt                                                                                       >  1:78732/1‑202 (MQ=255)                                                                                                                                                                                        aaGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCt                                                                                       <  2:78732/202‑1 (MQ=255)                                                                                                                                                                                        aaGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacac                                      >  1:113674/1‑251 (MQ=255)                                                                                                                                                                                         aGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacac                                      >  2:26053/1‑250 (MQ=255)                                                                                                                                                                                                tCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGGTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa                               <  1:367550/250‑1 (MQ=255)                                                                                                                                                                                                  gAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTGTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCGTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATAAGAGGa                            <  2:392853/251‑1 (MQ=255)                                                                                                                                                                                                   aaaCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGCGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCATCACACAATATGAGGAc                           >  2:357289/1‑251 (MQ=255)                                                                                                                                                                                                            gTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGCGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAAttt                  >  2:65326/1‑251 (MQ=255)                                                                                                                                                                                                                gACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAgg              <  2:65741/251‑1 (MQ=255)                                                                                                                                                                                                                   gAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGGACATACTCCACGTTTTTGCGGGCAGGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCTTACCAGCTGGTTAACTGTTGCTATTAAATAGCTCCTGGTTTTATCTTTTTCTTCGTAAACACGGAATAAATCGGCCTCACACAATTTTAGGTCGCCGATTTTTGGGGCg           <  2:292634/251‑1 (MQ=255)                                                                                                                                                                                                                        cGGCATTCTTGACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCGGTGGCTTCGTAAACACGTAAGAAAACGTCCTCACACAATATGAGGACGCCGAATGTTAGGGCGATgtc      <  2:22537/251‑3 (MQ=255)                                                                                                                                                                                                                          gCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGa    <  1:63028/251‑1 (MQ=255)                                                                                                                                                                                                                             tACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACCGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGaaa  >  1:217084/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               ATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAA  >  NC_000913/3474177‑3474646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑