Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,452,547	A→G	F127S (TTC→TCC)	rplC ←	50S ribosomal subunit protein L3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,452,547	0	A	G	100.0%	118.5 / NA	35	F127S (TTC→TCC)	rplC	50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

CGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGCACCGGTGGTCACCTGAATAGCA  >  NC_000913/3452300‑3452789                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   cGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGt                                                                                                                                                                                                                                                 >  1:187101/1‑251 (MQ=255)       cacaGCTGTTTTAACGATCAGGTCGCTACCGGTGGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑GACTACGTCAAGGCTCTTAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAgc                                                                                                                                                                                                                                           <  1:178509/251‑1 (MQ=255)       cacaGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAg                                                                                                                                                                                                                                            <  1:69239/250‑1 (MQ=255)            cTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGCCAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTACAGCGCtt                                                                                                                                                                                                                                       >  2:349764/1‑250 (MQ=255)                          aGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGa                                                                                                                                                                                                                         >  2:24805/1‑250 (MQ=255)                                  cccGGTGGCACCCGGGAAAGCACCTTTACCCGGAGGCAGTTGGCGC‑CCGCCTCCACGCGGTACTACGTCAAG‑CTCTGAAACGGTTACCCGTTCGTTACCCAATTGCCCTGCCTTTTCCTTGCCTT‑GAACCCCTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAacctttacc                                                                                                                                                                                                               <  2:218239/250‑1 (MQ=255)                                               gggACAGCACCTTTAACCAGCAGCCGGTTGCGCTCCGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTTAGACAAGGAGGTACCGGGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGAGcc                                                                                                                                                                                                   >  2:311509/1‑251 (MQ=255)                                                            tAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACt                                                                                                                                                                                      >  1:112025/1‑251 (MQ=255)                                                                            gcgcTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGCCAAGGAGTTACCGTGAGTAGCGTCCTGGGGACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTCCCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCa                                                                                                                                                                       >  1:229297/1‑250 (MQ=255)                                                                                 cagcttccaCGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTCCCCATCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGt                                                                                                                                                                 <  2:278365/243‑1 (MQ=255)                                                                                 cAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGt                                                                                                                                                                 >  1:31702/1‑251 (MQ=255)                                                                                       cAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTGCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACg                                                                                                                                                           <  1:374407/251‑1 (MQ=255)                                                                                                actactTCAAGGCTCTGAA‑CGGTGACACGGTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGTAAACAGTTCACCGCTAATGc                                                                                                                                                    <  2:420728/250‑1 (MQ=255)                                                                                                actacGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGc                                                                                                                                                    <  1:318682/250‑1 (MQ=255)                                                                                                     gTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGAc                                                                                                                                              <  1:99783/251‑1 (MQ=255)                                                                                                       cAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGAcc                                                                                                                                             <  2:31702/250‑1 (MQ=255)                                                                                                                gAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAAc                                                                                                                                   >  2:134623/1‑251 (MQ=255)                                                                                                                                cgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTCCCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGcc                                                                                                                    <  2:322063/251‑1 (MQ=255)                                                                                                                                cgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGc                                                                                                                     <  1:298824/250‑1 (MQ=255)                                                                                                                                 gttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGcc                                                                                                                    >  1:101284/1‑250 (MQ=255)                                                                                                                                   tACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAgg                                                                                                                 >  1:80621/1‑251 (MQ=255)                                                                                                                                              ccTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCca                                                                                                      <  1:389394/251‑1 (MQ=255)                                                                                                                                                   cATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGAc                                                                                                 <  2:101284/251‑1 (MQ=255)                                                                                                                                                                ttGAACACTTTGCCCGCAGTCTGCTTCTGACCGGTTGAACCCGGAACGCGGTGAGACCAGGAGTTACCGTGAGTAGCGTCCTGGGTAAGGGAGTTCCAGCGCTTACCGGCACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCATTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTc                                                                                    >  2:33535/1‑251 (MQ=255)                                                                                                                                                                       cTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGccc                                                                             >  2:81964/1‑250 (MQ=255)                                                                                                                                                                                 aggcTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGCCAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGa                                                                   <  1:405867/248‑1 (MQ=255)                                                                                                                                                                                  gTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTGTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGaa                                                                  <  2:187101/251‑1 (MQ=255)                                                                                                                                                                                                gATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTc                                                      >  2:271932/1‑249 (MQ=255)                                                                                                                                                                                                             aCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGGTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACg                                       <  2:113403/251‑1 (MQ=255)                                                                                                                                                                                                                  gTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGtt                                    >  1:336530/1‑249 (MQ=255)                                                                                                                                                                                                                            gAGTTACCGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGc                        <  1:134623/251‑1 (MQ=255)                                                                                                                                                                                                                                 cccGTGAGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCGTCGCCTTCAGCCAGGCGGAAGTCCCACATACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGCACCg                    <  2:22692/249‑1 (MQ=255)                                                                                                                                                                                                                                       aGTAGCGTCCTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGCACCGGTGGTCa             <  1:24805/251‑1 (MQ=255)                                                                                                                                                                                                                                                cTGGGTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGCACCGGTGGTCACCTGAAt      >  2:386360/1‑249 (MQ=255)                                                                                                                                                                                                                                                  gggTACGGGAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTGTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCTCCTTCAGCCTGGCGGAATTCCCACAGACCTCGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGCACCGGTGGTCACCTGAATAGCa  <  1:188228/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   CGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCG‑TACTACGTCAAGGCTCTGAA‑CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTTCAACGCTAATGCTCTGACCTACAGTGAACTCTTCGCCTTCAGCCAGGCGGAATTCCCACAGACCACGGCCAGCTTCTACGCCAGCTTTAGCGAAGTGGCCAGCTTCAGGCTTGGTCACACGGTTAGCTTTTTTAGCACCGGTGGTCACCTGAATAGCA  >  NC_000913/3452300‑3452789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑