Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	140.5 / NA	48	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/26);  total (22/26)

AGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTG  >  NC_000913/3387803‑3388266                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    aGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGcaac                                                                                                                                                                                                                         <  2:315167/251‑1 (MQ=255)      ggTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcg                                                                                                                                                                                                                    >  2:175082/1‑251 (MQ=255)          taACCGGCCAGCCCCCACGCATACCAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACACAAACCGGCCCAGATACAACACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAACGGCCGGACCGGCGGCAACAATccccgc                                                                                                                                                                                                                >  2:54849/1‑251 (MQ=255)               ggCCAGCCCCCACGCATACGAGTTTTCTATTCGTCCCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCCCACCAGAATCATCAATAATGGTGCGCGGATCACCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGtta                                                                                                                                                                                                           <  2:81613/251‑3 (MQ=255)                    gCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCAAACCAGAAGCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGGGCCGAGGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTAcc                                                                                                                                                                                                      <  1:255006/251‑1 (MQ=255)                        ccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCCCGATAGCGAATAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGCCAGTACCGccc                                                                                                                                                                                                  >  2:225760/1‑251 (MQ=255)                         cACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCa                                                                                                                                                                                                 <  1:632/251‑1 (MQ=255)                                  gAGTTTTCTATTCGTCCCAGCGAGGATATCCAGGTACAAAATCCGCCTCAGATACAGCACACCAGAATCATCAATAATGGGGCGCGGATCAGCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGt                                                                                                                                                                                        <  2:95128/251‑1 (MQ=255)                                      tttCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCCTCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTcc                                                                                                                                                                                    >  1:255288/1‑251 (MQ=255)                                                 aCCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGt                                                                                                                                                                          <  2:396249/250‑1 (MQ=255)                                                   cAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGaa                                                                                                                                                                       <  1:348784/251‑1 (MQ=255)                                                     gCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCTAGCTGGAAGTGAAAg                                                                                                                                                                     <  1:314180/251‑1 (MQ=255)                                                          gATATCCAGGTACAAAAAACGGCCCAGATACAGCACAACAGAATAATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATAAGGGTCACCTCCCGCAGCAAAGGCAGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTGTCCAGATGGAAGTGAAAGCcaac                                                                                                                                                                >  1:231628/1‑251 (MQ=255)                                                               ccAGGTACAAAACCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCaacaaac                                                                                                                                                            >  2:384763/1‑250 (MQ=255)                                                                 aGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCCGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATCGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGAAAAGCCCGGACCGGCGGCAACCATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCCGCTGGAAGTGAAAGCCAACAAACAgc                                                                                                                                                         >  1:187825/1‑251 (MQ=255)                                                                     aCAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGGACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCa                                                                                                                                                     <  1:225760/251‑1 (MQ=255)                                                                        aaaaCCGGCCCAGATACAGCACACCAGAAGCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTa                                                                                                                                                  <  2:244430/251‑1 (MQ=255)                                                                            ccGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAAt                                                                                                                                              <  1:399962/251‑1 (MQ=255)                                                                              ggCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATgg                                                                                                                                            >  1:119992/1‑251 (MQ=255)                                                                                           cacaCCAGAACCAGCAATAACGTGGCGCGGAGCACCGCAATGATGAGCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATTATGCGCAAAAGGCCACGATAGCGAATAGCGCCAGAATACGGTTCTCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTTGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGTCGGTCGCCAGttg                                                                                                                               <  2:239597/251‑2 (MQ=255)                                                                                            ccacCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCTCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGGAAGCCAACAAACAGCGCCATTACAATGGGGGTCGCCAGtttt                                                                                                                              <  2:201297/250‑1 (MQ=255)                                                                                            acacCAGACTCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAACAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCAGTCAGTACCGCACAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaacac                                                                                                                                                              >  2:298385/1‑218 (MQ=255)                                                                                            acacCAGAATCATCAATAATGGTGCGCGGAACATCGCAATGATGATCACTAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaacaa                                                                                                                                                              <  1:298385/219‑1 (MQ=255)                                                                                              acCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCAAGTACACTGGCGGTCGCCAGGTTTa                                                                                                                             >  1:372170/1‑250 (MQ=255)                                                                                                           ataatGGTGCGCGGATCAGCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGTGGTCGCCAGTTTTACCGCAAAGCGAa‑ta                                                                                                              <  2:252126/251‑1 (MQ=255)                                                                                                             aatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAa‑tat                                                                                                             <  1:175082/250‑1 (MQ=255)                                                                                                               tGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGACCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACCAACAGCGCCCGTACAATGGCGGTCGCCCATTTTGACGAAAATCGAA‑TATGtt                                                                                                          >  1:157676/1‑251 (MQ=255)                                                                                                                  tGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGCA‑TATGTTGGt                                                                                                       >  1:74715/1‑251 (MQ=255)                                                                                                                        gATCATCGCAATGATGATCACAAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCACCAATCGCCGCTGTCAGTACCGGCCAGCGTGGCGTTTCCAGCTGGAAGTGCAAGCCAACAAACAGCGCCAGTAAAATGGCGGTGGCCCGTTTTACCgacaagcagatcaggtggctagcac                                                                                                  >  2:87140/1‑227 (MQ=255)                                                                                                                                cAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGtt                                                                                                                                                                                       >  1:116464/1‑158 (MQ=255)                                                                                                                                cAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGtt                                                                                                                                                                                       <  2:116464/158‑1 (MQ=255)                                                                                                                                 aaTGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAATAAGCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑aaaa                                                                                       <  2:432554/251‑1 (MQ=255)                                                                                                                                    gatgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATa                                                                                     <  1:52832/250‑1 (MQ=255)                                                                                                                                        atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCa                                                                                 >  1:9315/1‑250 (MQ=255)                                                                                                                                          cACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCATCg                                                                              <  2:363545/251‑1 (MQ=255)                                                                                                                                           aCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCATCGt                                                                             <  1:126442/251‑1 (MQ=255)                                                                                                                                                       aTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGt                                                                                                       <  2:245009/214‑1 (MQ=255)                                                                                                                                                       aTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTACCGCAAAGCGAA‑TATGTTggg                                                                                                       >  1:245009/1‑213 (MQ=255)                                                                                                                                                                       tgCCGATGTTGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGGA‑TATGTTGGTTTGCAATGGAG‑AAAATAACCCTCGTGATTTACCAAACTCACGCCGGCGGTggg                                                 >  1:166374/1‑251 (MQ=255)                                                                                                                                                                            atgatgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCAtt                                            <  2:360036/251‑1 (MQ=255)                                                                                                                                                                                   gcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGga                                     <  2:74715/251‑1 (MQ=255)                                                                                                                                                                                    cAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCCCCCGGTTTTCCCCAAAACGAAATATGT‑GGTTAAGAATGGAG‑AAAATAACCATTGTGGTTAACCAAACTAACGCAGGCGGTGGGCCATTTTACgg                                      >  2:154079/1‑249 (MQ=255)                                                                                                                                                                                              cGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTAAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCACAAAACGAA‑TATGTTGGTTAGCCAAGGAGGAAAAT‑CCCATTGTGATTAACCCACCTACCGCAGGCGATGGGCCTTTTTAGCgaagaaaggatcc                          >  2:295626/1‑244 (MQ=255)                                                                                                                                                                                                aTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGCTTTACCGCAAAGAGGA‑TATGTTGGGTTGCAATGGAG‑AAAAAACCCAACGGGATTAACCAAACTCACACAGGCGCTGGGCCATTTTACGGGAGAAAGAATCCTg                        >  1:155005/1‑251 (MQ=255)                                                                                                                                                                                                  aGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAAGGGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTgg                       <  1:385554/250‑1 (MQ=255)                                                                                                                                                                                                     gAATAGCGCCAGAATACGGTTCCCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTATAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATTCCCATCGTGATGAACCAAACTCACGCAGGCTATGTGCCGTTTTACGGAAGAACGTATTCTGGCttt                   <  2:43765/251‑1 (MQ=255)                                                                                                                                                                                                            gcCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGCGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTTCCGCCAAGCGGA‑TATGTTGGTTAGCCAAGGGG‑AAAAAACCCCTCGTTATTTACCAAAATCACCCAGGCGAAGGGCCCATTTACGGGAGAACGAATCCCGGCTTTCGTcgcg            >  2:117583/1‑251 (MQ=255)                                                                                                                                                                                                                      ggTTCCCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTTGTTAGCAATGGAG‑AAAATACCCAACGTGATTTAACAAAATCACGCAGGAGATGGGGCATTTTTCGGGAGAACGAAACCTGGCTTTCGTCGCGATTTTGtttt  >  2:250183/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    AGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAA‑TATGTTGGTTAGCAATGGAG‑AAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTG  >  NC_000913/3387803‑3388266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑