Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,275,902	A→T	E207D (GAA→GAT)	garD →	GarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,275,902	0	A	T	97.3%	108.5 / NA	38	E207D (GAA→GAT)	garD	GarD
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (14/22);  minor base G (0/1);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAA‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCGG  >  NC_000913/3275657‑3276147                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          gCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGTTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAAGCATTGAACGCGATCTGCTACCGAAATACCCGAGCGTCGATGTCGTGGTGGGGCGGAATCATGTGTACGGTGGTGGCGTGGCGATGAACGCACCTGCGGCA‑GTTGTACCTATCCGTACCATGCACAATAGTGCGTTGAATCCTAACTTTGGCGGCGAG‑GTAAt                                                                                                                                                                                                                                                     <  2:422070/251‑1 (MQ=255) gCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTCAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAAAGCACCGGCGGCA‑GGTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTTGCGGCGAT‑GTAAt                                                                                                                                                                                                                                                     >  1:280215/1‑251 (MQ=255)          aGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTAACCATCCGTACCATTCACAATATTTCGCTGAATACTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTgg                                                                                                                                                                                                                                           >  1:127004/1‑251 (MQ=255)             gTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTTAAGCAACGGCGGCA‑GTTGTACCTCTCCGTACCAATCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATTGTG‑ATTGGCCt                                                                                                                                                                                                                                        >  1:40080/1‑251 (MQ=255)             gTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑TGAATGGTG‑ATTGGCCt                                                                                                                                                                                                                                        <  2:250400/251‑1 (MQ=255)             gTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCt                                                                                                                                                                                                                                        <  1:410651/251‑1 (MQ=255)                      aaaaaCCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAAAGCACCGGCGGCA‑GTTGTAACTTTCCGTGCCAATCACAATATTTCGCTGAATCCTAACTTTGGGGGCGTTTGTA‑TGGTG‑GTTGGCCTGGGtttgg                                                                                                                                                                                                                                >  2:98058/1‑247 (MQ=255)                           ccTGCTCGGTAGCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTTAATCATTTGTACGGGTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAAGCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCGTGGTTGTGAAAAGt                                                                                                                                                                                                                          <  2:388076/251‑1 (MQ=255)                                          caccaGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTTGCGGCGAT‑GGAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGCGCGcc                                                                                                                                                                                                           >  1:127917/1‑251 (MQ=255)                                             caGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGGAGTACAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGGGGTGGGGCTGAATCATTTGTACGGTTGTGGAGTGGCGATTAACGCACCGGCGGCATGTT‑TACCTTACCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGGGAT‑GTAATGGAGCATTGGC‑TGGGTTGTGAAAAATTGCAGccctgggtgctgg                                                                                                                                                                                                        >  2:136983/1‑240 (MQ=255)                                                 gTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑Ac                                                                                                                                                                                                   <  2:98211/251‑1 (MQ=255)                                                   ccACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTg                                                                                                                                                                                                 <  1:257071/251‑1 (MQ=255)                                                                     ggtggACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATAATTTGTACGGTTGTGGCGTGGCGATTTACGCAACGGCGGAA‑GGTGTATCTATACATACCATTTACAATATTTTGGTGAATCCTAAATTTGGCGGCGGT‑GTAATGGGG‑ATTTGCCTGGGTTGTGGAAAGTTGCAGCCTGAGCGCCTGCTGCAAGGGAA‑GGATGA‑TGTGCaa                                                                                                                                                                               >  1:55373/1‑250 (MQ=255)                                                                        gtaCTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTTGCGATTAACGCACCGGCGGCA‑GTTGTGCCTAGCCGTACGATTCACAATATTTCGCTGAATCCTAACTGTGGCGGGGAT‑GGAATGGTG‑ATGGGCGTGGGTTGTGAAGAGTTGCAGTCTGAGCGCCTGGGG‑ACGGGAACTGATGA‑TGTGCAAGCt                                                                                                                                                                            <  2:187582/248‑1 (MQ=255)                                                                              tGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTAACGCACCGGCGGCC‑GGTGTACCTATACGGACCATTAACAATATTTCGCTGAATCCTAACTTTGGGGGCTGT‑GTAATGGTG‑GTTTGGCTGGGTTGTGCAAAGTTGCAGCCTGGGGGCCTGCTG‑GCTGGGACGGGTGT‑GGGGCAAGCTATTCCa                                                                                                                                                                      >  2:210222/1‑250 (MQ=255)                                                                              tGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCC‑GTTGGACCTTTCCGTACCCTTCACAATATTTTGCTGAATCCTAACTTTTGCGGCGCA‑TTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCATCCTGAGGGCCTGCTG‑ACTGGGACGGGTGG‑TGTGGAAGCTATTcc                                                                                                                                                                       >  1:217421/1‑249 (MQ=255)                                                                                 agtaAAAATCATTGAACGCGATCTGCTACCGAAATACCCGACGGTCGATGTAGTGGTGGGGCTGAAGCATTGGTACGGGTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGTAACGGATGA‑GGTGCAAGCTATTCCAGTAg                                                                                                                                                                  <  2:368132/251‑1 (MQ=255)                                                                                                aCGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGgatga‑t                                                                                                                                                                                     <  1:32385/217‑1 (MQ=255)                                                                                                     aTCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GGTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGGT‑GTAATGGTG‑ATTTGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑GCTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAgg                                                                                                                                              >  1:117780/1‑250 (MQ=255)                                                                                                              cGAAATACCCGAACGTCGATGGCGTGGTGGGGCTTAATCATTTGTACGGTTGTTGCGTGGCGATTAACGCTCCGGCGGAA‑GTTGTACCTATCCGTCCCATTCACAATATTTCGCTTAATCCTAACTTTGGCGGCGAT‑GTAATTGCG‑ATTGGCCTGGCTTGTGAAAAGTTGCAGCCTGAGAGCCTGCTT‑ACTGGAACTGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATGGTCAGGTTGCGGGAt                                                                                                                                     <  2:252493/251‑1 (MQ=255)                                                                                                                 aagaCCCGAACGGCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGaa                                                                                                                                  <  2:374182/248‑1 (MQ=255)                                                                                                                  aTACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGAATAACGCACCGGCGGCA‑GAGTTAACTATCCGTACCCTTCCCAATATTTTGCTGAATCCTCACTTTGGGGGCGAT‑GGAAAGGGG‑ATTTGCCTTGGTTTTGAAAAGGTGCCGCCTGAGCGCCTGCTG‑ACTGGGACGGGTGGGTGGGCA‑GGTATTCCAGTAGAAAAGGCCCGCATTTTCAGTTTGCAGGATGaa                                                                                                                                  >  2:180394/1‑250 (MQ=255)                                                                                                                      ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCa                                                                                                                             <  1:98058/251‑1 (MQ=255)                                                                                                                                    cGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCAACGGCGGGA‑GTTGTACCTATCCGTACCATTTACAATATTTCGCTGAATCCTAACTTTTGCGGCGCT‑GTAATGGTG‑ATTGGCCTGGGGTGTGAAAAATTGCAGCCTGAGCGCCTGCTG‑ACTGGGACGGGTGG‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCCGTTTGCAGGGTGAAAAGCATGTCGGGTTTTAGt                                                                                                               >  2:253237/1‑251 (MQ=255)                                                                                                                                                     aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATAtt                                                                                              <  2:390554/251‑1 (MQ=255)                                                                                                                                                       ttGTACGGTTGGGGCGTTGCGATTAACTCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCGGAATCCTAACTTTTGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAAGTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATTA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTGGCAGTATGAAAGTCCTGTCGGTGTACCGGCCATGGTCGAGGATAttt                                                                                             <  2:278689/250‑1 (MQ=255)                                                                                                                                                                tgtgGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGgatga                                                                                                                                                                                       <  1:153396/152‑1 (MQ=255)                                                                                                                                                                   ggcgtggcgATTAACGCACAGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCg                                                                                 >  1:98825/1‑250 (MQ=255)                                                                                                                                                                     cgtggcgATTAACGCACCGGCTGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGGTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTGGAGGATATGTTGCAGATCGCCGAAc                                                                              <  2:218783/251‑1 (MQ=255)                                                                                                                                                                                aCGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTGTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTTCaa                                                                   <  2:107656/251‑1 (MQ=255)                                                                                                                                                                                 cGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACaaa                                                                  <  1:166600/251‑1 (MQ=255)                                                                                                                                                                                        gcggcA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGaa                                                           <  1:268145/251‑1 (MQ=255)                                                                                                                                                                                           gcA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCa                                                        <  2:217421/251‑1 (MQ=255)                                                                                                                                                                                             a‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAAc                                                      >  1:28610/1‑251 (MQ=255)                                                                                                                                                                                                  gTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCa                                                  <  1:433171/251‑1 (MQ=255)                                                                                                                                                                                                                        atatTTCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTc                             >  1:304052/1‑250 (MQ=255)                                                                                                                                                                                                                            ttCGCTGAATCCTAACTTTGGCGGCGAT‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGaa                         <  1:299900/250‑1 (MQ=255)                                                                                                                                                                                                                                                  ggcgAT‑GTAATGGTG‑ATTGGCCTGGGTTGTTAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCGTCAGAACTGGTCGTTGGTATGCAGTGCgg  <  1:143172/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          GCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCA‑GTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAA‑GTAATGGTG‑ATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTG‑ACTGGAACGGATGA‑TGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCGG  >  NC_000913/3275657‑3276147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑