Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,842	T→C	M300T (ATG→ACG)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,842	0	T	C	100.0%	85.2 / NA	26	M300T (ATG→ACG)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/21);  total (5/21)

GCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAG  >  NC_000913/3240606‑3241083                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   gCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGCAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGCAAGCGGTGTGTATGCCTTCTTCACCCGCCGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGCAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACggcgggacagccc                                                                                                                                                                                                                                       >  1:438216/1‑243 (MQ=255)  cgggTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAAGTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCGCCATCAAGACGGCGGGCGCGGCAAt                                                                                                                                                                                                                                     <  2:395474/249‑1 (MQ=255)    ggTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGGGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAAt                                                                                                                                                                                                                                     <  1:115327/249‑1 (MQ=255)          cTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGacgcggccaacccacttt                                                                                                                                                                                                                             >  2:425126/1‑237 (MQ=255)            gTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCTCAGCAATCAGTATTA‑c                                                                                                                                                                                                                           <  1:323625/250‑1 (MQ=255)                  tACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGtt                                                                                                                                                                                                                     <  1:372746/250‑1 (MQ=255)                    cTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACggcgggcggcgccaacactcttaacggggttac                                                                                                                                                                                                                   >  1:331019/1‑226 (MQ=255)                                                 ggtgtggtgGAAAATTCGTCTGAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCGCCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTAt                                                                                                                                                                                       <  2:130443/249‑1 (MQ=255)                                                                                      gTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCGATTCCGTTGCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCGGTTGAGCgtggt                                                                                                                                                <  1:387907/251‑1 (MQ=255)                                                                                      gTGCTGCTGTGCCTGCGCGAAAGCGGGGTGTATGCCTTCTTCACCCGCATCTCTGCAGCGAACATTCCGGGGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCgtggt                                                                                                                                                <  1:258052/251‑1 (MQ=255)                                                                                                      gcgAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGGGTGcc                                                                                                                                <  2:186675/251‑1 (MQ=255)                                                                                                                   gtaggCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAAGCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCg                                                                                                                   <  1:316191/247‑1 (MQ=255)                                                                                                                                   cGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtc                                                                                                   <  2:331624/251‑1 (MQ=255)                                                                                                                                     cAGCTCGGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAATCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCAGCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGATCGTGGTGGCTTCGCTGTGTGCCTTTTGCGCATCCGTCGTGGCGGGGGGGtctc                                                                                                 <  2:254152/251‑1 (MQ=255)                                                                                                                                       gCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCGCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctg                                                                                               <  2:331019/251‑1 (MQ=255)                                                                                                                                        ctctGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACCATTA‑TCGTGTTTTCGCTGGCTGCGGGTTATACGCTGGGGGTTTCGGTCGGTCTGCCCCCGGCGACGCTGTTGGGCGTGGTGGGTTCTCTTTGTGGCTGTGGGGCCTCCCGCGCggggggggggggtcttc                                                                                              >  1:28740/1‑244 (MQ=255)                                                                                                                                             cAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCTCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgctgctg                                                                                         <  1:396223/251‑1 (MQ=255)                                                                                                                                                    cATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGACGCACCCAACACTATTAACCG‑GTTTGCACTGGGTGGGGGTAATACGCTGGGGATTTCGGTGGGTCTTCCCACGGCGCGGCTGTTGAGCGGGGGGGGTTTTCTGTGTGCCCATGGGGCGTCCGGCGGGGGGGGGGGGgctcatcaggcgtttcccc                                                                                  >  1:145065/1‑236 (MQ=255)                                                                                                                                                        ccGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCGCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGCTCCCACTGGc                                                                              <  2:267902/251‑1 (MQ=255)                                                                                                                                                                                                      ccTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                                <  2:315211/251‑1 (MQ=255)                                                                                                                                                                                                      ccTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCGGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                                <  2:246507/251‑1 (MQ=255)                                                                                                                                                                                                              gTTTCTATTCCGCTGGGAGCCACCATCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg                        <  2:100785/251‑1 (MQ=255)                                                                                                                                                                                                                                   accaTCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCa   <  2:166428/251‑1 (MQ=255)                                                                                                                                                                                                                                   accaTCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCa   <  1:329066/251‑1 (MQ=255)                                                                                                                                                                                                                                   accaTCAATACGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCa   <  1:189740/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccaTCAATACGGCTGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTGAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGGTGAGCGTGGTGGCTTCTCGGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCGCTGCTGCTGATCCCACGGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGGTGCCGGCGGCTTTAGCATCGGCGTATTGCAg  <  2:220358/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   GCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTA‑CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAG  >  NC_000913/3240606‑3241083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑