Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	100.0%	112.8 / NA	34	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/21);  total (13/21)

CGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGG  >  NC_000913/968590‑969082                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cgGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAAtg                                                                                                                                                                                                                                                     <  1:239402/251‑1 (MQ=255)     aTTCGCGTGGGGGTCTTAGCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTGTCTGCCGTTCGTTCTGATGCGGTAAAAGCCAGTCTGGCGCAAGACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGa                                                                                                                                                                                                                                                 <  1:267641/251‑1 (MQ=255)        cgcgTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAt                                                                                                                                                                                                                                              <  1:63879/251‑1 (MQ=255)                           ggggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTTATGGtgtg                                                                                                                                                                                                                           <  1:355957/251‑1 (MQ=255)                            gggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGGCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGc                                                                                                                                                                                                                          <  2:103380/251‑1 (MQ=255)                                         aatgcggaattgtATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATACGCCTGTTGCGGTTTCGCCCGTTCGTTCTGATTCGGTAAAAGCCAGTCTGGCGCAACCCCCTGATGTGCAGATCAGCGTAACCGACGACGGTTTACAGCATTTCCGTCTGTCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGT‑TTTGGct                                                                                                                                                                                                             <  2:267546/238‑2 (MQ=255)                                                      aTCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCCACACACTGATGCGCCTGGTGCGGTTTCTCCCGTTCGTTCTGATGCGGGAAAAGCCATTCTGGCGCCACACCCTGATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGGCGC‑TTTTGCAATGGCTGGTGGTTg                                                                                                                                                                                               >  1:72697/1‑251 (MQ=255)                                                                aTTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAAATGTGTTGATTTTTTAACGCAATGATGCGCCTGTTGCGGGTTCTCCCGTTCGTTTTGATGCGGTAAAAGCCATTTTGGCGCAACAACCTGGTGTGCAGATCATCGTAACCGACGACGGTTTTAAGCATTACCGGCTGGCGCGTAATGGGGAAATTTGCGTTCTTGATGGTGTGAG‑GGCATTTGGCCATGGCTGGGGGTTTCCGggggggg                                                                                                                                                                                     >  1:249233/1‑250 (MQ=255)                                                                            tACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATCAACGCACTGATGCTCCTGTTGCGGTTTTTCCCGTTCGTTCTGGTTCGGTAAAAGCCATTTTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTCAAGCATTACCGTCTGGCGCGTAATGTGGAAATTTTAGTTTTTTGTGGTGTGCGGCGC‑TTTGGCAATGGCTGGGGGTTGCCGGCgggggcccatcgtgagg                                                                                                                                                                         >  1:190077/1‑238 (MQ=255)                                                                               cacaACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTgagcgag                                                                                                                                                                      <  1:232257/251‑1 (MQ=255)                                                                                            gCGGGTGATGAACCTGTGTTGAGTGATCATCGCAGTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAGCCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAGTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTaaa                                                                                                                                                         <  1:414436/251‑1 (MQ=255)                                                                                                  gatgaACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCggg                                                                                                                                                   >  1:128937/1‑250 (MQ=255)                                                                                                    tgaACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGCAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGCGCGGGCGGGGGGTTTACAGTCgggtg                                                                                                                                                 >  2:131806/1‑248 (MQ=255)                                                                                                                        aaCGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGAGGAGGTAAACGTCAAcggg                                                                                                                             >  1:365175/1‑250 (MQ=255)                                                                                                                         aCGCACGGATGCGCCTGTTGCGGTTTGTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGGCGTTATTGATGGTGGGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAAcggcg                                                                                                                            <  1:402524/251‑1 (MQ=255)                                                                                                                           gCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGCCGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAAcggcggg                                                                                                                          >  1:152324/1‑250 (MQ=255)                                                                                                                           gCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGgt                                                                                                                          <  1:263096/251‑1 (MQ=255)                                                                                                                                   gcgcCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCg                                                                                                                  <  1:311287/251‑1 (MQ=255)                                                                                                                                                 ttCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGCCGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGTGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGTCCCTCGCCGCGGTGGAATcc                                                                                                    >  1:63964/1‑251 (MQ=255)                                                                                                                                                      ccGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACGCCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATg                                                                                               <  1:399558/251‑1 (MQ=255)                                                                                                                                                       cgttcgttCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGGGCGAGCGGGGGGCTTAAAGTCGGGTGATGCGGGAATCGTCAACGGCGGGGTCCCTCGCCACGGTGGAATcccccatc                                                                                              >  1:302047/1‑247 (MQ=255)                                                                                                                                                              tcTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATctgc                                                                                        <  2:193183/250‑1 (MQ=255)                                                                                                                                                                            aGCCATTCTGGCGCAACTCCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCgg                                                                         <  2:374710/251‑1 (MQ=255)                                                                                                                                                                            aGCCATTCTGGCGCAACACCCTGATGTGCCGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGGGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGTCCCCCGCAGCGGGGGAATCCCCCTGCCACTGCTGCCGGGTccggggg                                                                         >  1:369813/1‑245 (MQ=255)                                                                                                                                                                                               ccTGATGTGCAGATCATCGTAACCGACGACTGTTTACAGCATGACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGTGTCAGGCGGTGAATTTCCGTGCCGTTAc                                                      <  2:36094/251‑1 (MQ=255)                                                                                                                                                                                                       gCAGATCATCGTAACCGGCGACGGTTTACAGCATTACCGGCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTtgtg                                              <  2:155891/251‑1 (MQ=255)                                                                                                                                                                                                             catcGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGtt                                         <  1:290660/250‑1 (MQ=255)                                                                                                                                                                                                                              gTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCCATGCGTGAGCGCGCGGGGCGCTTTAAGTCGGTTGATTCGGTAATCGTCAACGGCGGTGTCCCTCTCCGCGGGGGAATTCCCCTTCCTCTGCCTCCGGGTCCGGCGGTGACTTTTCGTACCGGTACGCGCTGTGACGTTTCTCCGCtttgacctttt                       >  1:141494/1‑242 (MQ=255)                                                                                                                                                                                                                                  aCAGCATTTCCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGGGCGTCGC‑TTGGGCAATGGCTGGTGGTTGCCGTCGGGGCCAATGCTTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGTTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAAGCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATGTACGGTCCGGTACGCGGTGTGACGTTGCTCAGCTTTAACATGTAGTgg                   <  1:376164/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAGCATTACCGTCTGGAGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGTCCCTCCCAGCGGGGGAATCCCCCAGCATCCGCTGCCGGGGCGGGCCGGGAAATTACGTACCCGGACGCGTTGTGGCGGTGCTCAGCTTTAACAAGTAGTggg                  >  1:327128/1‑250 (MQ=255)                                                                                                                                                                                                                                         tACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATgg             <  2:338505/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ggCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCg                                                                          <  2:243630/181‑1 (MQ=255)                                                                                                                                                                                                                                                 ggCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGGCCCTCGCAGCGGGGAAATCCCCATGCATCTGCTGCCggggccggcg                                                                          >  1:243630/1‑174 (MQ=255)                                                                                                                                                                                                                                                   cgcgTAATGTGGAAATTGTCGTTATTGATGGGGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTggg  <  1:433728/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CGGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGC‑TTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGG  >  NC_000913/968590‑969082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑