Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,423	C→T	G160G (GGC→GGT)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,423	0	C	T	100.0%	161.7 / NA	49	G160G (GGC→GGT)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/24);  total (25/24)

AAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGCTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATT  >  NC_000913/3240176‑3240650                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    aaaCCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCAGTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTc                                                                                                                                                                                                                                  <  2:193534/251‑1 (MQ=255)   aCCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGTCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTTATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTTGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGc                                                                                                                                                                                                                                <  1:413259/251‑1 (MQ=255)            cGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGCAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTc                                                                                                                                                                                                                        >  2:22650/1‑250 (MQ=255)               ccTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGACGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAACGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACgg                                                                                                                                                                                                                    >  2:280194/1‑251 (MQ=255)                  aTTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGTGATCGGCCTCTGTTTCGCACTGCGTCACGGt                                                                                                                                                                                                                   <  1:373098/249‑1 (MQ=255)                   ttttGTTCCTCTATCTACTGGGAACCTTCTCTGGTGCTCTGGCCGCAGTAGTATTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTCATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGTTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAAc                                                                                                                                                                                                                >  1:352643/1‑251 (MQ=255)                             ctatctaCTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCTTTTATCCATTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGaccac                                                                                                                                                                                                        <  1:20331/249‑1 (MQ=255)                              tatctaCTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGAccacca                                                                                                                                                                                                      >  2:321638/1‑250 (MQ=255)                                      gggCCCCTTCTCTTCTGCTCTGGCCGCAGTAGTCTTCAGCGTTGCCTTCCCTTCTACCCTGCATTTACCCAGTAGCGCGGGTGATATTTGGCCGCCATCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCCCCAAAAAccg                                                                                                                                                                                               <  2:241660/249‑2 (MQ=255)                                       ggCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGt                                                                                                                                                                                            >  1:130443/1‑251 (MQ=255)                                                 cttcggccctGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATGTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGAGGCGCGGGCTGGTAATGAGCATGGTGTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGata                                                                                                                                                                                    <  1:159988/241‑1 (MQ=255)                                                 ctgctgCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCATGCATTTATCCAGTAGCGCGAGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCAATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAACACTGGATAACGATATg                                                                                                                                                                                  >  1:428786/1‑251 (MQ=255)                                                   gctgctCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCAATGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGt                                                                                                                                                                                 <  1:169873/250‑1 (MQ=255)                                                   gctgctCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGCGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAACACCCTGGTTAACGATATGTc                                                                                                                                                                                >  1:254152/1‑251 (MQ=255)                                                     tgctCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGCGACCACCAAAAACCTGGTTAACGATATGTCg                                                                                                                                                                               >  2:389101/1‑250 (MQ=255)                                                               gCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTAc                                                                                                                                                                    <  2:2509/251‑1 (MQ=255)                                                                  gtagtCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCtt                                                                                                                                                                 <  1:201506/251‑1 (MQ=255)                                                                  gtagtCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCtt                                                                                                                                                                 <  1:316113/251‑1 (MQ=255)                                                                     gtCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTAt                                                                                                                                                              >  2:79820/1‑251 (MQ=255)                                                                            gCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGaa                                                                                                                                                        >  2:4606/1‑250 (MQ=255)                                                                                    ttcccttcTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCAt                                                                                                                                               >  1:137345/1‑251 (MQ=255)                                                                                      cccttcTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCAtt                                                                                                                                              <  2:219130/250‑1 (MQ=255)                                                                                             aCCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGCAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCttt                                                                                                                                                                >  2:289245/1‑225 (MQ=255)                                                                                             aCCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCttt                                                                                                                                                                <  1:289245/225‑1 (MQ=255)                                                                                                       tATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTgg                                                                                                                             <  1:325908/250‑1 (MQ=255)                                                                                                             gTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGCAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTACCGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTAttttt                                                                                                                      >  2:115327/1‑251 (MQ=255)                                                                                                                           atTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTttcttc                                                                                                        >  2:316191/1‑251 (MQ=255)                                                                                                                              tCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTa                                                                                                      <  1:395247/250‑1 (MQ=255)                                                                                                                                 ccgccgTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCt                                                                                                  <  2:319658/251‑1 (MQ=255)                                                                                                                                     cgTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTCTTGGGCTGGTTTCTTCTACCCTGGc                                                                                               >  1:37498/1‑250 (MQ=255)                                                                                                                                        cAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGGGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAacc                                                                                           >  1:227494/1‑251 (MQ=255)                                                                                                                                                 tCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGGTTCTTCTACCCTGGCAACCACCGGTTtc                                                                                  >  2:323625/1‑251 (MQ=255)                                                                                                                                                       tgatgaGCGGGCTGGTAATGCGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCt                                                                                                  >  2:429723/1‑229 (MQ=255)                                                                                                                                                       tGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCt                                                                                                  <  1:429723/229‑1 (MQ=255)                                                                                                                                                         atgcgcggggtgttattggtcATGGTTTCCAACCCCATCGACGCGCTGCGGGAAGGTAACGACATCGGGAGTCTGGTGTGGGCGATCGGCCGCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGGTATGTCGAATGCCGTTGCCTTTATGGTGAAACTGGTCATGCGCTTCGCCCCGATTGGTATTTTTGGGCTGTTTTCTTCTACCGTGGCACCCACCGGTTTCTCCACACt                                                                          <  2:70662/231‑1 (MQ=255)                                                                                                                                                              cgGGCTGGTAATGAGCATGGTTTCAAACCCAATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACAGCGTCACGGTAACGAGAACACCAAAAACCTGGTTACCGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGATTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTggg                                                                     >  1:380698/1‑251 (MQ=255)                                                                                                                                                                    ggTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACAGCGTGATTCTGGTGTGGGCGATCGGCCGCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAGTCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTa                                                                 <  2:104190/249‑1 (MQ=255)                                                                                                                                                                       aaTGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCACAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAAATGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACgcgc                                                            >  1:21240/1‑251 (MQ=255)                                                                                                                                                                        aTGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGTGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACgcgc                                                            >  1:33799/1‑250 (MQ=255)                                                                                                                                                                         tGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTCACTACCTCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTCTGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTCTTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCa                                                           >  1:395474/1‑250 (MQ=255)                                                                                                                                                                            gCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAActg                                                       <  1:218641/251‑1 (MQ=255)                                                                                                                                                                             cATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAActgc                                                      <  1:321638/251‑1 (MQ=255)                                                                                                                                                                                       aaCCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCt                                            <  1:389101/251‑1 (MQ=255)                                                                                                                                                                                               cGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGc                                     <  1:104592/250‑1 (MQ=255)                                                                                                                                                                                                      ctgctgAAAGGTAACTACATCGGAATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGCTTGGGATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGATGTTCGTGCTGGTTGGCTGTATGtt                             >  2:396223/1‑251 (MQ=255)                                                                                                                                                                                                          tgAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCCGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACtg                         >  1:304536/1‑251 (MQ=255)                                                                                                                                                                                                                       cATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCtggtgg            <  1:4606/251‑1 (MQ=255)                                                                                                                                                                                                                                tCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCAt   >  2:367058/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACGGTGTGGCTACGCTCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCAtt  <  1:28374/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    AAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGCTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATT  >  NC_000913/3240176‑3240650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑