Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,617	G→A	intergenic (‑28/‑77)	folB ← / → plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,617	0	G	A	100.0%	188.2 / NA	56	intergenic (‑28/‑77)	folB/plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

CCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGT  >  NC_000913/3204373‑3204860                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ccaccaGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATAcc                                                                                                                                                                                                                                               >  2:262222/1‑251 (MQ=255)  caccaGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGACCACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATAcc                                                                                                                                                                                                                                               >  1:417588/1‑250 (MQ=255)             aaCGCGCCCCCTCGACGTGGCTTACCCCCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGaa                                                                                                                                                                                                                                   <  1:11823/251‑1 (MQ=255)                     accTCTACGTGGCATACCACCGTTTCTGCAATGACAGCGTAACTGAGGCAATCCGCCACATCATCCATTTTCGCCGCTTTACGGTTAGCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGTTGGTCTGTTCCCCGTAGTAAACACCAATAGTGGGGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTCGACTAACCACATACCCCTTCCGGCAAAAtcatcgt                                                                                                                                                                                                                           >  2:4248/2‑245 (MQ=255)                        ccgatgTGGCTGACCCCCTTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGta                                                                                                                                                                                                                          <  2:343678/244‑1 (MQ=255)                             gtggctgaccgccttctcgacaaggTCAGCATAACTGAGGCAATCCGACACATCATCACTTTTCGCCGCGTTACTGTTATTCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGAGGGACTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCTAAAGGTGCTCTATAAATACAATATCCATCACGTCCTGCCTGTTTGTTGGCTAGCGAGATACCATTTCCGGCGAAAGGTGCGTAGTATc                                                                                                                                                                                                                     <  2:314823/225‑1 (MQ=255)                              tggatGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGACCACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATcaac                                                                                                                                                                                                                  >  2:204221/5‑248 (MQ=255)                              tGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGCTATCCCACCCCATTTCGATATCGCTCACTCACTTCTGTTCGCTGGCCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGCAAGTTGCTCTCTAAATCCAATATCCATCACGTCCTACCTGCTTTTTGGCTAACCAGATACCCCTTCCGGCGAAACGTGCGTATTATCcac                                                                                                                                                                                                                  >  1:425278/1‑251 (MQ=255)                               ggCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCcac                                                                                                                                                                                                                  >  2:354393/1‑250 (MQ=255)                                  tGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCcac                                                                                                                                                                                                                  >  1:365663/1‑247 (MQ=255)                                   gACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGAtt                                                                                                                                                                                                             <  1:170280/251‑1 (MQ=255)                                          gTTTCTGCAAGGTCAGCGTAACTGAGTCAATCCGCCACATCATCACTTTTCGCCGCGTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATAGCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAACGTGCGTATTATCCACAGATTCATCGtt                                                                                                                                                                                                      <  2:39557/251‑1 (MQ=255)                                               tGCAATGTCAGCGTACCTGAGGCAATCCGCCACATCATCACTTTTCGACGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGGTGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAATTTTGCGTATTCTCCACAGATTCATCGttacacc                                                                                                                                                                                                 >  2:87162/1‑246 (MQ=255)                                                gCAATGTCCGCGTATCTGAGGCATTCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCCATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCCTCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGAGTCATCGTTGAac                                                                                                                                                                                                  <  1:324302/249‑1 (MQ=255)                                                gCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGaa                                                                                                                                                                                                   <  1:419832/248‑1 (MQ=255)                                                        aGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTc                                                                                                                                                                                        <  2:339911/251‑1 (MQ=255)                                                                 gAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGaa                                                                                                                                                                               <  2:242883/251‑1 (MQ=255)                                                                   ggCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAAc                                                                                                                                                                              >  1:328691/1‑250 (MQ=255)                                                                         ccGCCACATCATCCCTTGTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTGCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTAGAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATTCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTa                                                                                                                                                                        <  1:212136/250‑1 (MQ=255)                                                                                     caCTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAACCGGAACAGCTTATGAGTGCAATcgc                                                                                                                                                           >  2:370686/1‑251 (MQ=255)                                                                                           tCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTg                                                                                                                                                      <  1:437955/250‑1 (MQ=255)                                                                                                gcTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGGCCTGCCTTCTTTTTGGCTAACCAGATACCACGTCCGGCGAAATGTGCGTATTAGCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGATCAGCTTATGAGTGCAATCGCGCCTGGAATGa                                                                                                                                                <  2:165478/251‑1 (MQ=255)                                                                                                    tACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATcc                                                                                                                                             <  1:227604/250‑1 (MQ=255)                                                                                                       ggTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCAt                                                                                                                                         >  2:233502/1‑251 (MQ=255)                                                                                                        gTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATc                                                                                                                                        <  2:152171/251‑1 (MQ=255)                                                                                                                aCGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGACAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACct                                                                                                                                >  2:213607/1‑251 (MQ=255)                                                                                                                  gCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGa                                                                                                                                                                     <  1:203539/212‑1 (MQ=255)                                                                                                                  gCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTATGTTCCCCGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTACGGCGCAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGa                                                                                                                                                                     >  2:203539/1‑212 (MQ=255)                                                                                                                     aTTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCg                                                                                                                           >  1:10527/1‑251 (MQ=255)                                                                                                                         cGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCCGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTc                                                                                                                       >  2:27499/1‑251 (MQ=255)                                                                                                                                   cacTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGt                                                                                                             >  1:27299/1‑251 (MQ=255)                                                                                                                                         catctggtcgcaggTCTGTTCCCAGTAGTAAACACCAATAGTGGTGCTTACCGATAGTTGCTCTCTAAATACAATATCCATCACGTCCTGCCTGCTTTATGGCTAACCAGATACCACTTCCGGCGAACTGTGCGTATTATCCACAGATTCATCGTTGAACACGACTTTTCACAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGTCAtt                                                                                                       >  2:53247/13‑251 (MQ=255)                                                                                                                                              gTTCGATGGTCTGTTCCCAGTCGTAAACTCCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGt                                                                                                  <  1:419723/251‑1 (MQ=255)                                                                                                                                                     ggTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCg                                                                                            <  2:10527/250‑1 (MQ=255)                                                                                                                                                            tCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTtgtgtg                                                                                    >  1:268720/1‑251 (MQ=255)                                                                                                                                                             cccAGTCTTAAACACCAATATTGGTGATTACCGAAAGTTTGTCGATAAATACAATATCCAGCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGGGCGTATTATCCACAGAGTCATCGTTGAACACGAATTTTCAAAACGGATCAGCTTATGAGTGCAATCGCGTCGGTAATGAGCCTCATCGCCTTCCTGGGCGGCTGCATTGCCAGGGCCATTCTGGGGTGCCGCTcgtgtg                                                                                    <  2:66061/250‑1 (MQ=255)                                                                                                                                                                 gTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTg                                                                               <  1:137070/251‑1 (MQ=255)                                                                                                                                                                          cccAATAGTGGGGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACATCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCcg                                                                      <  1:204221/250‑1 (MQ=255)                                                                                                                                                                                  tggtgATTACCGAAAGTTGCTCTATAAATACAATATCCATCGCGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAAcc                                                                >  2:297632/1‑249 (MQ=255)                                                                                                                                                                                          aCCGAAAGGTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTcc                                                       <  2:113890/250‑1 (MQ=255)                                                                                                                                                                                                    gCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAgg                                            >  2:379128/1‑251 (MQ=255)                                                                                                                                                                                                      tctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgcg                                          >  1:219415/1‑251 (MQ=255)                                                                                                                                                                                                       ctATAAATACAATATCCATCACGGCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTAGCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgc                                           <  2:119041/249‑1 (MQ=255)                                                                                                                                                                                                            aaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAAcc                                     >  1:342176/1‑250 (MQ=255)                                                                                                                                                                                                            aaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAAcc                                     >  2:188812/1‑250 (MQ=255)                                                                                                                                                                                                                cAATATCCATCACGTCCTGCCTGCTTTTTTGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAAtgt                                <  2:340963/251‑1 (MQ=255)                                                                                                                                                                                                                      ccATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACCGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCCGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACg                          >  2:435557/1‑251 (MQ=255)                                                                                                                                                                                                                        aTCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAACCGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGt                         >  1:270745/1‑250 (MQ=255)                                                                                                                                                                                                                                     tgcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAACCGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGCTCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAggg           >  1:417897/1‑251 (MQ=255)                                                                                                                                                                                                                                     tgcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAggg           >  1:176668/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGagc        <  1:354393/250‑1 (MQ=255)                                                                                                                                                                                                                                         ttttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGag         <  1:27499/249‑1 (MQ=255)                                                                                                                                                                                                                                         ttttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGag         <  1:411014/249‑1 (MQ=255)                                                                                                                                                                                                                                           ttGGCTAACCAGATACCACTTCCGGCGAAATGTGCTTATTATCCACAGATTCATCGTTGAACACGAATTATCAAAACGGAACAGCTTATGAGCTCAATCGCGCCTGGAATGATCCTCACCGCGTCCCTCTTCGGCTCCACTTCCAGGGCCATCCTGGTTTGCGGCTTGTGTTGGCTGCCCGATCCGCGAACCATGGGCTCCGGCAAGCCAGGCGCAACCAATGTGTTACggtactgtggcgagttagcat      <  2:157307/250‑21 (MQ=255)                                                                                                                                                                                                                                            tGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGcc    >  2:240703/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCACGGGAGCAGCCGt  >  1:272616/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGT  >  NC_000913/3204373‑3204860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑