Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,625	G→A	S70F (TCC→TTC)	cpdA ←	cAMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,625	0	G	A	98.2%	184.8 / NA	54	S70F (TCC→TTC)	cpdA	cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (19/34);  minor base C (1/0);  total (20/34)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACC  >  NC_000913/3176383‑3176867                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   gTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATcc                                                                                                                                                                                                                                            <  1:360832/251‑1 (MQ=255)      cGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTgcgc                                                                                                                                                                                                                                       <  1:120279/251‑1 (MQ=255)           cAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAAt                                                                                                                                                                                                                                  <  2:393931/251‑1 (MQ=255)           cAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAAt                                                                                                                                                                                                                                  >  1:41131/1‑251 (MQ=255)                aCTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCGAAATCACCt                                                                                                                                                                                                                             >  2:37511/1‑251 (MQ=255)                   cAAGCTGAAACTCGCTCAGCTCTCCGTGCGGCACGCCAAACACCTGGCGATCCAGCAACAGGAGTTGCCATTGCTCTCCAATAAACACGCGCTTCGCCGGGGATATACCCGCATCCTGTATCGCGCTGTACATCGCGGGCTGGAAATCGTGGGTGCCCGGCAGCCTGA‑CGCAGGGCGTACGAAAACTTGCGATGCCTTCATCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCTCTAAATCGCCTGTc                                                                                                                                                                                                                          <  1:295452/251‑1 (MQ=255)                   cAAGCTGAAACTCGCTCAGCGCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACATGTc                                                                                                                                                                                                                          <  2:404947/251‑1 (MQ=255)                    aaGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTcg                                                                                                                                                                                                                         <  2:326917/251‑1 (MQ=255)                        tGAAACTAGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGAGAAATGCTGATAGGCCGCAGAGCATTGATCCTGCGCTAAATCACCTGTCGCGAc                                                                                                                                                                                                                     >  2:245467/1‑251 (MQ=255)                                                ggCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGgtg                                                                                                                                                                                             <  1:391117/251‑1 (MQ=255)                                                  cACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGAGCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGtgtg                                                                                                                                                                                           <  1:367055/251‑1 (MQ=255)                                                   aCGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTgg                                                                                                                                                                                          >  1:261698/1‑251 (MQ=255)                                                    cGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGc                                                                                                                                                                                         >  2:47193/1‑251 (MQ=255)                                                     gCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATGCGTGCTGGTGTGGcc                                                                                                                                                                                        <  1:331035/251‑1 (MQ=255)                                                         aaCACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAAt                                                                                                                                                                                    <  1:343416/251‑1 (MQ=255)                                                               tGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTc                                                                                                                                                                              >  1:316263/1‑251 (MQ=255)                                                                  cTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATACCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCAGAATCGCCTcc                                                                                                                                                                             >  1:293239/1‑249 (MQ=255)                                                                        aGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCAGATTCGCCTCCAGCACCGc                                                                                                                                                                     >  1:371005/1‑251 (MQ=255)                                                                          cAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCCCCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCt                                                                                                                                                                   <  1:233016/251‑1 (MQ=255)                                                                             cAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGt                                                                                                                                                                >  1:434202/1‑251 (MQ=255)                                                                                gatatGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGACACAAGCACCCTGTAACGCGCTGTGCATGACGCGCTGCAAATCGTGGTTACCAGGCAGCCCGA‑AGCAGGCCGATCGCAACCTTGAGATGCCTTCAGCGACATGCTCATCGGGCGCAGAGAATTGACCCTGCGCTAAACCATCTGTCGCGACAATCAGGTCGAATTCGTGCTGGCGAGGACGAATCGCCTCCAGCACCGCCTGGGAAc                                                                                                                                                             >  1:259211/5‑251 (MQ=255)                                                                                    tGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAActct                                                                                                                                                          <  1:229391/250‑1 (MQ=255)                                                                                           gCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGgt                                                                                                                                                   <  2:122694/250‑1 (MQ=255)                                                                                                     aaactcccgcTTCGCCTGGGAAATACCCGTGTCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGTCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCTCCTGTCGCGACAATCAGGTCGAATTCGTGCTGTTGTGGCCGAATCGCCTCCAGCACCTCCTGGTAACTCTCCCAGGTGTTTACCCCTa                                                                                                                                        <  1:281239/244‑1 (MQ=255)                                                                                                     aaaCCCGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTa                                                                                                                                        <  1:281339/251‑1 (MQ=255)                                                                                                      aaCACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTaa                                                                                                                                       <  1:47193/251‑1 (MQ=255)                                                                                                              cTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGCTAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGCATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCACAGGTGTTTACCCCTAACAGGGCtt                                                                                                                               >  2:141971/1‑251 (MQ=255)                                                                                                                tCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTc                                                                                                                              <  2:356851/250‑1 (MQ=255)                                                                                                                      gggaaaaccccGCATCCCGTAACGCGCTGTACAGCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCC‑GATCGCAGGGCGCACGAGAACTTGCTATGCCTTCAGCGAAACGCTGATAGGCCGGAGAGAAATGATCCTGCGCTAAATCACCTTACGCTACAAGCAGGTGGAATGCGTGCTGGTGTGGCCGAATCGCCGCCAGTTCCGCCTGGTAACTCTCCCAGTTTTTTACCCCTGACaagtctgcgtgcgtt                                                                                                                       <  2:215922/243‑15 (MQ=255)                                                                                                                        gagaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTtg                                                                                                                     <  1:336752/251‑1 (MQ=255)                                                                                                                         agaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGACAACTTGCCATGCCTTCAGCGAAATGCTGCTAGGCAGCAGAGAATTGATCCTGCGCTAAATCACCTGTAGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                                    >  1:434391/1‑251 (MQ=255)                                                                                                                           aTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                                                  >  1:410348/1‑251 (MQ=255)                                                                                                                             aCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCa                                                                                                                 <  1:27184/250‑1 (MQ=255)                                                                                                                                  cATCCTGGAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCCCCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAgg                                                                                                           <  2:366810/251‑1 (MQ=255)                                                                                                                                        ggaaCGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAgt                                                                                                      <  2:183296/248‑1 (MQ=255)                                                                                                                                            cgcgCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGTCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCgg                                                                                                 <  2:389189/251‑1 (MQ=255)                                                                                                                                                                      cGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGcc                                                                       <  1:97002/251‑1 (MQ=255)                                                                                                                                                                         ggTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTcc                                                                    >  2:44992/1‑250 (MQ=255)                                                                                                                                                                                   cAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagccag                                                            >  2:432585/1‑249 (MQ=255)                                                                                                                                                                                       cAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCCCCTGTCCCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGTGCTGCGTGCGTTTGTGCAAACAGGTGATTGGCGGTAATTTGGAAAATCCCGACTCTGGCCTCTCCAGCCAGAGGaat                                                      <  2:387320/251‑2 (MQ=255)                                                                                                                                                                                       cAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAg                                                      <  2:223692/251‑1 (MQ=255)                                                                                                                                                                                         gA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAggg                                                    <  1:188620/251‑1 (MQ=255)                                                                                                                                                                                             gCAGGGCGCACGAAAACTGGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCGCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGGGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAATGGTTa                                                 <  2:263400/251‑1 (MQ=255)                                                                                                                                                                                                gggCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACa                                              <  1:413917/251‑1 (MQ=255)                                                                                                                                                                                                    gcACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGCCAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTg                                                                          >  2:187354/1‑219 (MQ=255)                                                                                                                                                                                                    gcACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTg                                                                          <  1:187354/219‑1 (MQ=255)                                                                                                                                                                                                                  gATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTc                            >  1:339085/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGttt                   <  2:48595/251‑1 (MQ=255)                                                                                                                                                                                                                             cGAAATGCTGATATGGCGCAGAGAATTGATCCTGCGCTAAATCCCCTGTCGCGACACTCCGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTACCTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCATACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGCGGAAGGGTTAACAGGCTTTCCACATGGTGTCCTTAGGTTTcc                 >  2:250288/1‑250 (MQ=255)                                                                                                                                                                                                                               aaaTGCAGATAGGCCGCAGAGAATTGATACTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGAGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTCCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGAAAAATCATGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAgggtacacg               >  2:192313/1‑244 (MQ=255)                                                                                                                                                                                                                                 aTGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGCCGCGACAATCAGGTCGAATTAGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCacgac             >  1:196741/1‑251 (MQ=255)                                                                                                                                                                                                                                         aGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTGTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa     <  2:261698/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCGAATAAAcc  <  1:368449/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccGCAGAGAATTGATCCTGCGCTAAATCACCGGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAcc  <  2:125016/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   GTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGA‑CGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACC  >  NC_000913/3176383‑3176867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑