Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,216	G→A	Q152* (CAG→TAG)	ftsP ←	cell division protein FtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,216	0	G	A	100.0%	174.4 / NA	53	Q152* (CAG→TAG)	ftsP	cell division protein FtsP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/26);  total (27/26)

GTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGT  >  NC_000913/3161973‑3162449                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          gTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACCCCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGcc                                                                                                                                                                                                                                    <  2:226066/251‑1 (MQ=255)        gcgcACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGt                                                                                                                                                                                                                             <  1:218003/251‑1 (MQ=255)         cgcACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGtt                                                                                                                                                                                                                            <  1:210151/251‑1 (MQ=255)              ccAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAg                                                                                                                                                                                                                       <  1:331057/251‑1 (MQ=255)                      gcgAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCg                                                                                                                                                                                                               >  2:99636/1‑251 (MQ=255)                                aaCGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACcaca                                                                                                                                                                                                     <  1:89731/251‑1 (MQ=255)                                     aCGGGCGTTGTACGCCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACGTCATCTGCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTa                                                                                                                                                                                                <  2:221266/251‑1 (MQ=255)                                        ggCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCt                                                                                                                                                                                             <  2:383846/251‑1 (MQ=255)                                             tGTACCCCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGtt                                                                                                                                                                                        >  2:197832/1‑251 (MQ=255)                                                aaacCGTTAACAAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCCGCCGTTTATCCTGGATAATGACAGGAAAATAATCAACACCATAATGGATGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGACGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGAGACAACAGAGTAGCTGCGTTCTg                                                                                                                                                                                     >  2:189635/3‑251 (MQ=255)                                                           cagcagCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGGTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCAGCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCGCATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTGTGGCGAATGGGCa                                                                                                                                                                          <  2:82489/251‑1 (MQ=255)                                                              cagcGTACCAACAACAAAAACGCAGCTTCACGGATCGTTGTGTACTGGCGTCCCACAGTTATCCAGCCGTTTATCCTGGATAATGTCCGGAAAATGATCCACACCATAATGATTGGGGATAGGCAGCGACTTGCTGACTTACTCTTCCACCAGCTACACTCCGGCAAGGCAGTTATAGAACTACTGTGCCATGCGGTATGGCGGATTGGCGTGATACAACGGAGTAACTGCGTTCTGGCAAATGGGCAgg                                                                                                                                                                        >  2:284123/1‑249 (MQ=255)                                                                    aTCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGCTAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTTGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCGGTACGGGTg                                                                                                                                                                 >  2:100861/1‑251 (MQ=255)                                                                      cACCAACAAATCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAAGGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTgt                                                                                                                                                                <  2:347282/250‑2 (MQ=255)                                                                            caaAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                                                                                                                                                         <  1:332325/251‑1 (MQ=255)                                                                                 ccgccgCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTTAGCGtt                                                                                                                                                    <  2:118856/251‑1 (MQ=255)                                                                                    ccgcTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTgg                                                                                                                                                 >  1:142636/1‑251 (MQ=255)                                                                                             ggTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCCCACCATAATGGTTGGTTATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACAGTCCGGCAAGGCCGTGATAGACCTACTGAGCCGTGCGGTAGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTGCTGGTGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatcat                                                                                                                                        <  1:260852/251‑1 (MQ=255)                                                                                                           tGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGc                                                                                                                          >  1:353091/1‑251 (MQ=255)                                                                                                                   cAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGc                                                                                                                  <  1:88821/251‑1 (MQ=255)                                                                                                                         tATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCt                                                                                                            >  1:149598/1‑251 (MQ=255)                                                                                                                           tCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGAGCCCCGTCAGCGTTTGGCGACATCATCCGTGCCGGACAGCCCATCAGCGGGCCTgg                                                                                                          >  1:66770/1‑251 (MQ=255)                                                                                                                                ccGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCt                                                                                                     >  1:84418/1‑251 (MQ=255)                                                                                                                                    ttATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGt                                                                                                   >  1:175783/1‑249 (MQ=255)                                                                                                                                        ccTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACca                                                                                                                                                                                                       <  2:76610/145‑1 (MQ=255)                                                                                                                                        ccTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACca                                                                                                                                                                                                       >  1:76610/1‑145 (MQ=255)                                                                                                                                        ccTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCg                                                                                             >  1:152465/1‑251 (MQ=255)                                                                                                                                           ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGCGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGcc                                                                                          >  2:90939/1‑251 (MQ=255)                                                                                                                                           ggATAATGACCGGAAAAGCATCCACACCATAATGGGTTGGGATAGGCAGCGACTTGCTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTACGGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCgg                                                                                            <  2:194317/249‑1 (MQ=255)                                                                                                                                              tAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCg                                                                                                                                                                                  >  1:152313/1‑160 (MQ=255)                                                                                                                                              tAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCg                                                                                                                                                                                  <  2:152313/160‑1 (MQ=255)                                                                                                                                                    ccGGAAAATCACCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAtt                                                                                 <  2:413225/251‑1 (MQ=255)                                                                                                                                                      ggAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTg                                                                                >  1:29171/1‑250 (MQ=255)                                                                                                                                                       gAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTg                                                                                >  1:240463/1‑249 (MQ=255)                                                                                                                                                       gAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATATTCCACCAGCCACATTCAGGCAAGGACGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACAACAGAGTAGCTGCGTTCTgg                                                                                                                                                                                    >  1:31268/1‑149 (MQ=255)                                                                                                                                                       gAAAAGCATCCTCACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCATCCACATTCCGGCAAGGCCGTTATAGACGTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTgg                                                                                                                                                                                    <  2:31268/149‑1 (MQ=255)                                                                                                                                                        aaaaTCATCCACACCATAATGGTTGGGGATAGACAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGAACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTg                                                                                >  1:317567/1‑248 (MQ=255)                                                                                                                                                             catcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAttt                                                                        >  1:349802/1‑251 (MQ=255)                                                                                                                                                               cccACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCCCATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTc                                                                      <  1:103242/250‑1 (MQ=255)                                                                                                                                                                   cacCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTc                                                                  >  1:205223/1‑251 (MQ=255)                                                                                                                                                                       agaaTGGTGGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCTCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGc                                                              <  2:351092/249‑1 (MQ=255)                                                                                                                                                                        gaaTGGTTGGGGATAGGCAGCGATTTGCTGCCTTCAGCTCCCACCAGCCACCTTCCGGCAAGGCCGTTATAGACCTACTTAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCCGGTACCTGTAGACCGGCCACCGTCAGTGAGACAATTTCTGTCAGGCg                                                             <  2:71825/250‑1 (MQ=255)                                                                                                                                                                          aTGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTAACTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                           >  2:39793/1‑251 (MQ=255)                                                                                                                                                                            ggTTGGGGATAGGCGGCGACTTGCGGACTTCATCCTCCACCAGCCACAGTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGGCCGCGTTTGGCGACATCTTCCGTGCCGGACCGCCCAGCAGCGGGCCTGGGACCTGGAGCCCGGCCACCGTCATTGAGACCTTTTCTGTCAGTCGGTTg                                                         <  2:419888/251‑1 (MQ=255)                                                                                                                                                                                              aCTTGCTGACTTCAGCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACa                                       <  2:344856/251‑1 (MQ=255)                                                                                                                                                                                                          cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCtt                            <  2:349802/250‑1 (MQ=255)                                                                                                                                                                                                               tCCACCAGCCACATTCCGGCAAGGACGTTATAGACCTACTGAGCCCTGCGGTTGGGAGTATTGGCGTGATACAACAGTGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACCTCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCCGGCGGTTGCTGTAAATAAGCTTACCATCGTCGCCCTTCCAGAc                      >  2:222266/1‑251 (MQ=255)                                                                                                                                                                                                                        cacaTTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGt             >  1:298534/1‑251 (MQ=255)                                                                                                                                                                                                                        cacaTTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGCTGGGAGTATTGGCGTGATACCACAGAGTAGATGCGTTCTGGCGAATGGGCAGTACGGGGGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGGAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATatt             >  2:56338/1‑249 (MQ=255)                                                                                                                                                                                                                           aTTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCgg          >  1:314694/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGt  <  1:303972/251‑1 (MQ=255)                                                                                                                                                                                                                                           tataGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATaa                                               <  2:354476/198‑1 (MQ=255)                                                                                                                                                                                                                                           tataGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATaa                                               >  1:354476/1‑198 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          GTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGT  >  NC_000913/3161973‑3162449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑