Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,263	G→A	A45T (GCC→ACC)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,263	0	G	A	100.0%	114.8 / NA	35	A45T (GCC→ACC)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/12);  total (23/12)

CAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTAC  >  NC_000913/3134019‑3134502                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                cAACCTTATTGTGACAATAGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAAGTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGGGAACATCTTATTACCGTTGTCGAAGAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGaa                                                                                                                                                                                                                                           <  1:62053/251‑1 (MQ=255)                                        gAATTACGCTTTCATAAAAACATATTAACCAAAAAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGACGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCAGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACAGTTGATGGGa                                                                                                                                                                                                     >  1:91886/1‑250 (MQ=255)                                                 tttCATAAAAACATATTACCCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTAc                                                                                                                                                                                           >  1:42719/1‑251 (MQ=255)                                                                  aaCCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACg                                                                                                                                                                           >  2:389585/1‑250 (MQ=255)                                                                    ccAAATAAATATTTTTACTGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGaa                                                                                                                                                                         >  1:133880/1‑250 (MQ=255)                                                                     cAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGACGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAAt                                                                                                                                                                       >  2:351642/1‑251 (MQ=255)                                                                       aataaataTTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAAAACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACACAATTACCGTTGATGGGAAAATCCTTACATAAAACAAtt                                                                                                                                                                                >  2:75505/1‑240 (MQ=255)                                                                       aataaataTTTTTAATGGATAATTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAAtt                                                                                                                                                                                <  1:75505/240‑1 (MQ=255)                                                                                          tatTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCaaaaaa                                                                                                                                                   >  1:303527/1‑250 (MQ=255)                                                                                                  ttaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTgg                                                                                                                                          >  2:98965/1‑251 (MQ=255)                                                                                                  ttaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTgg                                                                                                                                          >  2:298964/1‑251 (MQ=255)                                                                                                             atTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCgg                                                                                                                               <  1:80026/251‑1 (MQ=255)                                                                                                              ttCATGCAGCCAACCACACCTCCATTAATTGCCGTTCTTGGTCGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGCTGTCGAACCATCTGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGCAGTGCAGCGACAAAATTACCGTTGATGGAAACATCCTTAACTAAACCAATTGAACGAAATCAGGTGACAACAGCAAAAAAATTGCCTGGACCAGCTCCGgc                                                                                                                              >  2:203480/1‑251 (MQ=255)                                                                                                                      gTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTa                                                                                                                       >  2:161916/1‑250 (MQ=255)                                                                                                                            aaCACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACa                                                                                                                 <  1:98965/250‑1 (MQ=255)                                                                                                                                ccTCCATTCATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCACCGGTGTGTGAACTTCTTATTACCGTTGTCGAAAACTATGGGGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACAAGTTCCGGCGCTGGTAATTACAGCGtt                                                                                                            >  2:50059/1‑251 (MQ=255)                                                                                                                                   ccATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTGCCGGCGCTGGTAATTACAGCGTTTg                                                                                                          <  2:349176/250‑1 (MQ=255)                                                                                                                                    cATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTc                                                                                                        >  1:63403/1‑251 (MQ=255)                                                                                                                                       tAATTGCCGTTATTGGTAGCGATGGGTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACAGAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAAGTACAGCGTTTGTCGc                                                                                                      <  2:63403/250‑1 (MQ=255)                                                                                                                                        aaTTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATgg                                                                                                                                                                                                       <  1:110467/152‑1 (MQ=255)                                                                                                                                        aaTTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATgg                                                                                                                                                                                                       >  2:110467/1‑152 (MQ=255)                                                                                                                                         aTTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTTCCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCa                                                                                                                                      <  1:66431/216‑1 (MQ=255)                                                                                                                                         aTTGCCGCTATTGGTAGCGATGGTTCAGGCAAGTCCACGGTGAGTGAACATCTTATTACCGTTGTCGAAAAATATGGCGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGCAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCa                                                                                                                                      >  2:66431/1‑216 (MQ=255)                                                                                                                                                           gATGGTTCAGGCCAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGCAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAAAAGACCGGAAATGTAGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGAGCTGGTAATAACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCg                                                                                 >  2:437016/1‑251 (MQ=255)                                                                                                                                                             tGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTc                                                                               >  1:297502/1‑251 (MQ=255)                                                                                                                                                                       aaGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGc                                                                     >  1:191988/1‑251 (MQ=255)                                                                                                                                                                                              tattaCCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTg                                              >  1:400493/1‑251 (MQ=255)                                                                                                                                                                                                 taCCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTc                                           >  2:188681/1‑251 (MQ=255)                                                                                                                                                                                                   ccGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTa                                         <  2:85254/251‑1 (MQ=255)                                                                                                                                                                                                            aaaaaTATGGTGCTGCCGCAAGAGTTCCTTTAGGAAAACAGACCGGAACTGGCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATCAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACAAGTTCCGGCGCTGGTCATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGCCGCGGGTTAATTGTTCTAACCGACCGt                                >  2:41116/1‑251 (MQ=255)                                                                                                                                                                                                                    ggTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGTTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTc                         <  1:197356/250‑1 (MQ=255)                                                                                                                                                                                                                     gTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTc                         >  1:433116/1‑249 (MQ=255)                                                                                                                                                                                                                                     tCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGgc        <  2:297502/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCt     <  2:400493/251‑1 (MQ=255)                                                                                                                                                                                                                                          tAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGAGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTAc  >  1:157386/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTAC  >  NC_000913/3134019‑3134502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑