Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,133,175	G→A	T13I (ACC→ATC)	yghR ←	putative ATP‑binding protein YghR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,133,175	0	G	A	100.0%	126.9 / NA	40	T13I (ACC→ATC)	yghR	putative ATP‑binding protein YghR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/25);  total (15/25)

AGATTTGGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGG  >  NC_000913/3132928‑3133388                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        agaTTTGGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTg                                                                                                                                                                                                                      <  1:302550/251‑1 (MQ=255) agaTTTGGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGGCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTg                                                                                                                                                                                                                      <  2:71659/251‑1 (MQ=255)  gaTTTGGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGt                                                                                                                                                                                                                     <  1:288467/251‑1 (MQ=255)        gTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTAtt                                                                                                                                                                                                               <  1:317356/251‑1 (MQ=255)            tCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTCCCGGTGCCATCACCCCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAAc                                                                                                                                                                                                            >  2:387912/1‑250 (MQ=255)              aTGCTTTGGGTTTTCGAGGGTTTGGCCGCCAGTAGCAGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGCTTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTCACagt                                                                                                                                                                                                         >  2:253131/1‑251 (MQ=255)                         tttCGGGGATTTGGGAGCCAGTAGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTGTCCGTCTTGGCCGTAGAGCAGACCCAGATAGCGCCGCGCGGTTTGCCAGGGTTGTTGCAGCGATGTCACCAGGTCGGTGGGCAGTGTGGAGTTACCGGGGCCATCACACCCGACCACCGCAATCAGGCCCGGAATGTAGTTGGGCTGCGGCGCGATGGTACTATTAACAGTTTGAgtgtgt                                                                                                                                                                                               <  2:280880/250‑1 (MQ=255)                                     ggCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTGCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCAtt                                                                                                                                                                                   <  1:246678/250‑1 (MQ=255)                                       ccgccAGTCGTCGGTCCAGCCAGTCGCCAACCAACGGCAATCTTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGACAGTGTTGGTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCCCCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGTGATTGTACTATTAACAGTTTGAGTTTGTAGAGCATCCATTCGt                                                                                                                                                                                <  2:222124/251‑1 (MQ=255)                                               cGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACagtttgagt                                                                                                                                                                                                   >  1:125910/1‑224 (MQ=255)                                               cGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACagtttgagt                                                                                                                                                                                                   <  2:125910/224‑1 (MQ=255)                                                gCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCCTTCGTTAAatcatc                                                                                                                                                                       >  2:267266/1‑251 (MQ=255)                                                    ttCCAGCCAGACGCCAACAAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCt                                                                                                                                                                   >  2:188440/1‑251 (MQ=255)                                                            agACGCCAACCAACGGCCCTCGTTTGATTTTGTCGCCCTCTTCGCCGGAGAGCAGCCCCCGACAGCGACGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACAAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCACCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAttt                                                                                                                                                           >  2:183295/1‑251 (MQ=255)                                                                          ggCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGaaa                                                                                                                                             <  1:81876/251‑1 (MQ=255)                                                                             aaTCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAgcg                                                                                                                                          <  2:225008/251‑1 (MQ=255)                                                                                   ttGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGTCGCGATTGTACGATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTGTGGTGATCAGGAAAGcgcg                                                                                                                                        <  1:416448/247‑1 (MQ=255)                                                                                   ttGATTTTGACGCCGTCTTCGCCGGAGAGCCGCAACAGATAGCGCCGCTCGGTTTGCCGGTGTTGTTGCAGCGCTTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACAACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGAGCGATTGTACTATTACCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGcgcg                                                                                                                                        >  2:416448/1‑247 (MQ=255)                                                                                              gCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCCCCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGt                                                                                                                         >  2:24114/1‑251 (MQ=255)                                                                                                     tCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGTCGCGATTGTACTATTAACAGTTTGAGTGTGTAGTGCATCCATTCGTGAAATCATCCCCTATCCATTTTGGTGATCAGGAAATCGCGGCGGGAATTGCGCGTAGTg                                                                                                                     <  2:67654/248‑1 (MQ=255)                                                                                                     tCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTg                                                                                                                     <  2:135429/248‑1 (MQ=255)                                                                                                                     ccAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATc                                                                                                  <  1:199846/251‑1 (MQ=255)                                                                                                                          tAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGCATTGCGCGTAGTGACGCTTGCAGAATTTCATcggcgg                                                                                             >  2:286327/1‑251 (MQ=255)                                                                                                                                 gcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCACGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCg                                                                                     <  1:258337/251‑1 (MQ=255)                                                                                                                                    cGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTTGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCTTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑cc                                                                                  <  1:294174/250‑1 (MQ=255)                                                                                                                                                 ttgttgCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACCGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTAATCGGCGGGGTG‑TCGGCCCA‑CCAGtttcagg                                                                    >  2:320312/1‑246 (MQ=255)                                                                                                                                                  tgttgCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGG‑TCG‑CCCATCCAGTTCAAgg                                                                    >  1:78501/1‑250 (MQ=255)                                                                                                                                                   ggtgCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGAt                                                                  <  2:120399/249‑1 (MQ=255)                                                                                                                                                           gATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGG‑CCC‑TCCCGTTCAAAGATCTTTtcgc                                                          >  1:159901/1‑251 (MQ=255)                                                                                                                                                           gATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGG‑TAG‑CCCATCCAGTTCAAGGATCTTTgcgc                                                          >  1:361656/1‑251 (MQ=255)                                                                                                                                                              ttCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGTCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATGTTGGTGATCAGGAAAGCGCGGCGGGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCa                                                        <  1:176149/250‑1 (MQ=255)                                                                                                                                                               tCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATGTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCAt                                                       <  1:207949/250‑1 (MQ=255)                                                                                                                                                                             gtCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgcg                                        <  1:388521/251‑1 (MQ=255)                                                                                                                                                                                   gtgGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGTGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTaa                                  <  1:49415/251‑1 (MQ=255)                                                                                                                                                                                         ttACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGG‑ACG‑CCCATCCAGTTCCAGGATCTTTTCGCCATTTAATGGCGGTTGCGGGGGAACGGCgg                            >  2:237183/1‑250 (MQ=255)                                                                                                                                                                                                            cGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCTATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCTGCGTAACGGCGATTTTTTCCTGTAACGCTGc         <  1:369809/251‑1 (MQ=255)                                                                                                                                                                                                             gACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCAGTCGTTAAATCATCCCCTATCCATTTTGTTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGTCGATTTTTTCCTGTAACGCTGcc        <  2:12215/251‑1 (MQ=255)                                                                                                                                                                                                                caccGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGG‑GCG‑CCCCTCCAGTTCAAGGAACTTTGCGCCATTGAATGTCAGTTGCGGCGTAAAGGCGGTTTTTTTCTGTTACGCTGCCAgg     >  1:393355/1‑250 (MQ=255)                                                                                                                                                                                                                  ccGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtg   <  1:295699/251‑1 (MQ=255)                                                                                                                                                                                                                   cGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCGGTtgg  <  1:117457/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        AGATTTGGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTG‑TCG‑CCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGG  >  NC_000913/3132928‑3133388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑