Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,127,981	G→A	P14L (CCC→CTC)	glcD ←	glycolate dehydrogenase, putative FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,127,981	0	G	A	100.0%	221.7 / NA	66	P14L (CCC→CTC)	glcD	glycolate dehydrogenase, putative FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/40);  total (26/40)

ACCAGAAAGCCCGGTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGC  >  NC_000913/3127743‑3128224                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    accaGAAAGCCCGGTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCAt                                                                                                                                                                                                                                         <  1:7709/251‑1 (MQ=255)  ccaGAAAGCCCGGTGCCGGCACCCCGGGTCACCCCCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTACATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACGCGTAAGGAATGATCGCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATc                                                                                                                                                                                                                                        <  1:236499/251‑1 (MQ=255)       aaGCCCGGTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGAc                                                                                                                                                                                                                                   <  2:272112/251‑1 (MQ=255)       aaGCCCGGTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTGGCGTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACGCGTAAGGAATGATCTCCTCATCGGTATGCAGGAGCTCAAGTCCAGGGACATGCTCACGCAGTGCCAGCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGAc                                                                                                                                                                                                                                   <  1:221314/251‑1 (MQ=255)            cGGTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGTACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGctct                                                                                                                                                                                                                              <  1:417108/251‑1 (MQ=255)             ggTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCCCCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCtt                                                                                                                                                                                                                             <  2:96358/251‑1 (MQ=255)                   tGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTAc                                                                                                                                                                                                                        >  2:335394/1‑250 (MQ=255)                                        tACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTc                                                                                                                                                                                                  >  1:221508/1‑251 (MQ=255)                                             gCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCttt                                                                                                                                                                                             <  1:376402/251‑1 (MQ=255)                                                   gaaggcggagagctaGAATCGCTTTTGCCTGTTCCATTTGCTTAGGCAGAACAGCCAGTAACGGACGCGTGCTATACGCGCTCAACCCGTCACGCGCGTAAGGAATGATCTCCTCATCGGTATGCAGTATCTCAAGTCCAGGGACGTGCACACGCAGTGCCATCAGTACCGATGTGCGGTTGACAGCGAGTAAAGCGCCATCAAGACGCTCTTCGTACCAGATGCTCATGAGTAGTCTTCGCTTtgttgtt                                                                                                                                                                                       <  2:245978/237‑2 (MQ=255)                                                   gATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTtgttgtg                                                                                                                                                                                       <  1:129388/251‑1 (MQ=255)                                                    aTGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACAGCGAGTAAAGCGCAATCAAGACGCTCTGCGTACAAGATGCTCATGAGTAGGCTTCGCTTtgttgtgt                                                                                                                                                                                      <  2:2789/251‑1 (MQ=255)                                                                cAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCt                                                                                                                                                                          <  1:83459/251‑1 (MQ=255)                                                                 aGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAATCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTg                                                                                                                                                                         <  2:75937/251‑1 (MQ=255)                                                                  gAATCGCTGCTACCCGTTCCATTTGCTTCGGCCGCACAACCAGTAACGGCCGCGCGCGATACGCGCTCACCCCATCAAACTCGTAAGGACTGCTCTCCTCATCGATATGCAGGATCTCAAGACCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCCTGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTg                                                                                                                                                                         >  2:406469/1‑250 (MQ=255)                                                                    aTCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCGCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGAtt                                                                                                                                                                      <  2:191231/251‑1 (MQ=255)                                                                       gCTGTCACCTGTTCAATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCCGCTGAttttt                                                                                                                                                                   >  2:48527/1‑251 (MQ=255)                                                                         tGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAGGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGCGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTgc                                                                                                                                                                 >  2:308539/1‑251 (MQ=255)                                                                            cACCTTTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTgcgcg                                                                                                                                                              <  2:269313/251‑1 (MQ=255)                                                                             aCCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTgctcgc                                                                                                                                                             >  2:66724/1‑251 (MQ=255)                                                                                 gTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCgctg                                                                                                                                                           <  1:66886/249‑1 (MQ=255)                                                                                     cATTTGCTTAGGCAGAACAACCAGTAATGTACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtg                                                                                                                                                     <  2:73878/251‑1 (MQ=255)                                                                                              aGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGt                                                                                                                                             <  1:156449/250‑1 (MQ=255)                                                                                              aGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGt                                                                                                                                             <  1:371498/250‑1 (MQ=255)                                                                                              aGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCACAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGtt                                                                                                                                            <  1:66724/251‑1 (MQ=255)                                                                                               ggCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGtta                                                                                                                                           <  1:335395/251‑1 (MQ=255)                                                                                                gCAGAACAACCAGTAATGGACGCGTGAGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGATTCTGTGACCAGttat                                                                                                                                          >  2:400798/1‑251 (MQ=255)                                                                                                      caacCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCg                                                                                                                                    <  2:180207/251‑1 (MQ=255)                                                                                                          cAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCt                                                                                                                                >  2:429252/1‑251 (MQ=255)                                                                                                           aGTAATGGACGCGTGCGATACGCGCTCAACACGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGCCGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCtt                                                                                                                               >  2:405597/1‑251 (MQ=255)                                                                                                                 ggACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgtt                                                                                                                         <  1:328472/251‑1 (MQ=255)                                                                                                                      cgTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTtttcgttttcgt                                                                                                                    <  1:340514/251‑1 (MQ=255)                                                                                                                           gATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTAtc                                                                                                               >  2:17589/1‑251 (MQ=255)                                                                                                                                      aaCCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTAc                                                                                                    >  2:217799/1‑251 (MQ=255)                                                                                                                                          cGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGt                                                                                                >  2:112272/1‑251 (MQ=255)                                                                                                                                          cGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTGCGTCTATCTCTTTAGCTACCGGt                                                                                                <  1:112077/251‑1 (MQ=255)                                                                                                                                            tCACACTCGTAAGGACTGATCTCCTCATCGGTATGCCGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCCGTACCGATGTGCGGTCGACATCGAGTAACGCGCCATCAAGACGCTCTTCGTCCAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTc                                                                                               >  2:170844/1‑250 (MQ=255)                                                                                                                                             cacaCTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCTCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCa                                                                                              <  1:191592/250‑1 (MQ=255)                                                                                                                                               cacTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGGGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGAc                                                                                           >  2:324491/1‑251 (MQ=255)                                                                                                                                                acTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGAcc                                                                                          >  1:176591/1‑251 (MQ=255)                                                                                                                                                   cGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtt                                                                                       <  1:7020/251‑1 (MQ=255)                                                                                                                                                    gTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttt                                                                                      >  1:176315/1‑251 (MQ=255)                                                                                                                                                        ggAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttttttt                                                                                  >  1:7355/1‑251 (MQ=255)                                                                                                                                                         gAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttt                                                                                 <  2:48024/251‑1 (MQ=255)                                                                                                                                                         gAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttt                                                                                 <  1:17589/251‑1 (MQ=255)                                                                                                                                                         gAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGAACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttt                                                                                 >  2:319473/1‑251 (MQ=255)                                                                                                                                                                  cctcGTCGGTATGCAGGAGCGCAAGTCCAGGGACATGCTCACGCATGGCCATCAGTAGCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTGCAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTGGTGTGGCAGCTTATTTTTGCGCGCTGCTTCTTTGAACAGTTATTAAGCTGGTTTATCGGTTTCGTCTATCTCTTTAGCTACCGGTCAGTCCATTTTTTTTCCAGc                                                                            <  2:211381/247‑1 (MQ=255)                                                                                                                                                                  cctcATCTGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGct                                                                           <  2:378856/248‑1 (MQ=255)                                                                                                                                                                  cctcATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCGTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCACTGTAGCTACCGGTGAGACCATTTTTTTTCCAGct                                                                           <  2:24077/248‑1 (MQ=255)                                                                                                                                                                    tcATCGGTATGCCGGATATCCAGTCCACGGATATGCTCACGCAGTGCCATCCGTACCGATGTGCGGTAGACATCGAGTAACGCGCAATCAAGACGCTCTTCGTAGAAGATGATCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCCGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGAGGGCTTTTCGTTTTCGTCTATATCTTTAGCTACTGGTCAGACCGTTTTTTTTACAGCTCtgtg                                                                      >  2:256768/1‑251 (MQ=255)                                                                                                                                                                    tcATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACaa                                                                                                                                                                                                                      >  1:369459/1‑107 (MQ=255)                                                                                                                                                                    tcATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACaa                                                                                                                                                                                                                      <  2:369459/107‑1 (MQ=255)                                                                                                                                                                        cGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGCCCt                                                                  <  2:331128/251‑1 (MQ=255)                                                                                                                                                                               cAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTg                                                           <  1:48527/251‑1 (MQ=255)                                                                                                                                                                                      tcAAGTCCCGGGACATGCTCACGCAGTGCCATCAGTACCGCTGTGCGGACGACATCGAGTAACGCGCCATCAAGCCGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGATGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACt                                                    >  2:55492/1‑251 (MQ=255)                                                                                                                                                                                        aaGTCCATGGACATGCTCACGCAGTGCCATCAGTACCGATGGGCGGTCGACATCGAGTAAAGCGTCATCAAGACGCTTTTCGGACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGTCTTTTCGTTTGCGTCTATCTCTTGAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCa                                                  <  2:16916/251‑1 (MQ=255)                                                                                                                                                                                        aaGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGGGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGTTCTTTTCGTTTGCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCGCTGTGACCTTGGCTTGGTTAACTCa                                                  <  2:414318/251‑1 (MQ=255)                                                                                                                                                                                         aatCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACCAGTTGCTCATGGGTAGGCTTCGCTTTGTTGTGTTGTGGGGCAGCTGCTTTTTGCGAGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGATACCGGTCAGCCCATTTTTTTTCCAGATATGTGACCTTTTCTTGGTTAACtac                                                  >  2:347269/3‑248 (MQ=255)                                                                                                                                                                                                ggACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAg                                                                                             >  2:330123/1‑200 (MQ=255)                                                                                                                                                                                                ggACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAg                                                                                             <  1:330123/200‑1 (MQ=255)                                                                                                                                                                                                         cACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGt                                   <  1:16391/249‑1 (MQ=255)                                                                                                                                                                                                             cAGTGCCATCAGTACCGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACAt                              >  1:128154/1‑250 (MQ=255)                                                                                                                                                                                                                            cGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGAtg              >  2:232737/1‑251 (MQ=255)                                                                                                                                                                                                                            cGATGTGCGGTCGACATCGAGTAAAGCGCCATCAAGACGCTCCTCGTACAAGATGCTCATGAGTATGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTGGCGCGCTGTTTCTGTGAACAGGTATTAAGCGGGCTTTTCGTTTTCGACTATCTCTTTAGCTACCGGTCATACCATTTTTTGTCCAGCTCGGGTACCTTGTCTTGGTTAACGCAATGTTAAATTAAGCTAACATAAGCACTTGCGTGAtg              <  2:400476/251‑1 (MQ=255)                                                                                                                                                                                                                                     gTCGACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGGCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGttt     <  1:429252/251‑1 (MQ=255)                                                                                                                                                                                                                                        gACATCGAGTAAAGCGCCATCAAGACGCTCTTCGTACAAGAGGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATGTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGc  >  1:157153/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    ACCAGAAAGCCCGGTGCCTGCACCACGGGTCACCACCGGTACACGCAGGCGATGGCAGACAGCCAGAATCGCTGTCACCTGTTCCATTTGCTTAGGCAGAACAACCAGTAATGGACGCGTGCGATACGCGCTCAACCCGTCACACTCGTAAGGAATGATCTCCTCATCGGTATGCAGGATCTCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGC  >  NC_000913/3127743‑3128224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑