Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,121,033	T→C	I83V (ATT→GTT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,121,033	0	T	C	100.0%	71.8 / NA	21	I83V (ATT→GTT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/14);  total (7/14)

ATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAAC  >  NC_000913/3120788‑3121276                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    atcggCAATCAGCCACACCCCCGCCGCGTATAACGGTTTGAACCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCAc                                                                                                                                                                                                                                                 <  1:137621/247‑1 (MQ=255)    ggCAACCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCCCCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTa                                                                                                                                                                                                                                             <  1:97846/251‑1 (MQ=255)         ccggccccaGCCCCGCCGCGTATAACGTTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGc                                                                                                                                                                                                                                        <  1:152506/244‑1 (MQ=255)          aaGACACACCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGcc                                                                                                                                                                                                                                       <  1:14376/250‑1 (MQ=255)                  gCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATcc                                                                                                                                                                                                                                >  2:312259/1‑250 (MQ=255)                                             ccAGGCACACCAGGAGCGTACCGGTCCTCGCCACCGGCGCACCAACGCCAGACGCTCCTTCCAGCAACGCACCAACGGAGAACTCAATCAGTACCACCTGCAAACGCTGATCGTCGGTGATGGAGCTAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGCCAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATATCCCgcagcagc                                                                                                                                                                                                     >  2:390755/1‑250 (MQ=255)                                                       cagcagCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGCGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCatat                                                                                                                                                                                          >  2:420565/1‑251 (MQ=255)                                                       cagcagCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCatat                                                                                                                                                                                          <  1:168780/251‑1 (MQ=255)                                                                 ccGGTAAACGCCCCCGGCGCACAAAAGCCAGCCGCTCCTTCCAGCAACGCACAAAAGGAGAAACCAATAAGTAACCCCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATAGCGAACTGCCCGCTGGCAACGGTTAATTTATAAAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCAt                                                                                                                                                                                <  1:38432/251‑1 (MQ=255)                                                                                                 cgcccctcccAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAAAATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGgatagata                                                                                                                                                <  1:275065/243‑1 (MQ=255)                                                                                                  gCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGgatagata                                                                                                                                                >  1:362183/1‑250 (MQ=255)                                                                                                      cTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCa                                                                                                                                                             >  2:260801/1‑233 (MQ=255)                                                                                                      cTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCa                                                                                                                                                             <  1:260801/233‑1 (MQ=255)                                                                                                                            gaAACCAATAAGTAACAACTGCAAACGCTGATCGTCGGAGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGGTAATTTATAAAGGAACACCGCCGAGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGGTATTGCTCCAGCGACAt                                                                                                                     >  2:146383/1‑251 (MQ=255)                                                                                                                              aaCCAATCAGTAACACCTGCAAACGCTGTTCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACGGCCCGCTGGCACCGGTTAATTTATACAGGACCACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGt                                                                                                                   <  2:98137/251‑1 (MQ=255)                                                                                                                                                                          aTAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAAtattat                                                                       >  1:92864/1‑251 (MQ=255)                                                                                                                                                                              ccGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCggg                                                                   <  1:214940/251‑1 (MQ=255)                                                                                                                                                                               cGAGCTGCGGATAATAACGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTAGCGGGa                                                                  <  2:314538/251‑1 (MQ=255)                                                                                                                                                                                                                                          ccgcGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAg       <  2:92864/251‑1 (MQ=255)                                                                                                                                                                                                                                            gcgACAATAACCCACGCTATTGGCAATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGTGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGTGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGt     <  1:261537/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCAATAACCCACGCTATTGGCCATAATCCAGAAATAAAGCCATAGCCCGCAGCATCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTTCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCAGCGGCATATACATTTGGGTCCAGGTAAc  <  1:219513/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAAC  >  NC_000913/3120788‑3121276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑