Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,786	G→A	T165I (ACT→ATT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,786	0	G	A	100.0%	122.0 / NA	36	T165I (ACT→ATT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/23);  total (13/23)

ATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTT  >  NC_000913/3120546‑3121006                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             atatAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAAt                                                                                                                                                                                                                    <  1:301112/251‑1 (MQ=255)               aaaGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGcccc                                                                                                                                                                                                       <  1:241537/250‑1 (MQ=255)                 aGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCCCGTCTCTTTCCCCCCTTCCCAGCCGTCCATCATTCCTCCCAGCCAGAACGGCACAAGACCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCccgcc                                                                                                                                                                                                    <  1:16169/251‑1 (MQ=255)                            aCAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCCCCGGCGCCATATTGGCACTCAGACACAGCCCCGCCGCGGATAACgg                                                                                                                                                                                         >  2:38432/1‑251 (MQ=255)                                   aGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAg                                                                                                                                                                                  <  1:31273/251‑1 (MQ=255)                                            cAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACAAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGccc                                                                                                                                                                         >  2:76264/1‑251 (MQ=255)                                              gCAACCAGCCCCGCTGCCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATGGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCccac                                                                                                                                                                       <  2:45893/251‑1 (MQ=255)                                                aacccgccccgcGGGCCCCGTCTCTTTCACCCCTTTCCGCCCGTCATTCATTGTTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGCCGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAAGCAGTCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCcca                                                                                                                                                                        <  2:141433/240‑1 (MQ=255)                                                aaCCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGACcca                                                                                                                                                                        >  1:141433/1‑248 (MQ=255)                                                             ggCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGt                                                                                                                                                        >  2:138584/1‑251 (MQ=255)                                                                       ttttCACCCCTTTCCAGCCGCCCTTCATTGCTACCGGCCAGACCGGCACAAGAACCGACAGGAACGGTAACTGCCTTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCTCAATATTGGCAGTCAGACACAGGCCCGCCGCGTATAACGGTTTGAAGTCCAGGCCCATCAGCAGCGCGCCGTTAATCGCCa                                                                                                                                                <  2:401515/248‑1 (MQ=255)                                                                       ctTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGcc                                                                                                                                                 <  1:301710/248‑1 (MQ=255)                                                                                           tCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGc                                                                                                                          >  2:29015/1‑251 (MQ=255)                                                                                                   tgcaaCCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGCCGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTcc                                                                                                                   <  1:198095/246‑1 (MQ=255)                                                                                                             cagAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCAcc                                                                                                        >  1:314538/1‑251 (MQ=255)                                                                                                                         aGAACCGACAGGAACGGTAACTGCCGTCCCGCCATTGCGCCAATGGGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAcc                                                                                            <  1:76264/251‑1 (MQ=255)                                                                                                                            aCCGACAGGAACGTTAACTGACTTCCCGCCATGGCGCCAATGTGGAACGGATCGATTCCCGTTACCGGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGa                                                                                                                                                                                                                <  1:63633/132‑1 (MQ=255)                                                                                                                            aCCGACAGGAACGGTAACTGACGTCCCGCCCTTGCGCCCATGTGGCACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGa                                                                                                                                                                                                                >  2:63633/1‑132 (MQ=255)                                                                                                                                        gggaaCTGACGTCGAGCCATTGCGCCAATGTGGAACGGATCGCTTCCCGTTCCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAGCCGACACCGGCGCAATATTGGCAATCAGCCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCCCCAGCACCGCACCGGCACTCGCCCCCGGCGCCCCAAAGCCAGCCGGTCCTTCCAGCAACGCCCCCAAGGCGAAACCAATCAGTCACCCCTg                                                                             >  2:182783/4‑251 (MQ=255)                                                                                                                                              tGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCa                                                                                                                                                                              >  1:256745/1‑148 (MQ=255)                                                                                                                                              tGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTGGAAGCCCa                                                                                                                                                                              <  2:256745/148‑1 (MQ=255)                                                                                                                                                 cGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTg                                                                    <  1:109989/251‑1 (MQ=255)                                                                                                                                                        ccATGGCGCCAATGGGGAACGGAGCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACACCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGCCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCGGAtcgtcg                                                             <  2:10000/251‑1 (MQ=255)                                                                                                                                                             gcgcCAATGTGGAACGGATCGATTCCCGTTACCTGCCCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCTCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGAt                                                        <  1:128418/251‑1 (MQ=255)                                                                                                                                                                   aTGTGGAACGGATCGATTTCCGTTTCCTGACCGGCAACTAGACGCGGCACGCCCACCGCACCATACGCCACCGGAGCAATAGTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCGCCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGGACCAAAGGAGAAACCAATCAGTAACTCCTGCAAACGCTGATCGTCGGTGATGGAGAt                                                  <  2:394778/251‑1 (MQ=255)                                                                                                                                                                     gtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATTGAGATa                                                 <  1:108944/250‑1 (MQ=255)                                                                                                                                                                       ggAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAAcc                                              <  1:138584/251‑1 (MQ=255)                                                                                                                                                                         aaCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACTAAACGCCACCGGCGCAATATTGGCATTCATACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCTCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCGCCTTCCATCAACGCACCAAAGGAGAAGCCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGa                                            <  1:182783/251‑1 (MQ=255)                                                                                                                                                                          aCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCCCCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTCACACCTGCAAACGCTGATCGTAGGAGCTGGAGATAACCg                                             >  2:214940/1‑249 (MQ=255)                                                                                                                                                                                     accGTTACCTGACAGAAGACCAGAATCGGCACGCCCACCGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCAGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAACGAGAAACCAATCCGAAACACATGCCCACCCTCATCGACGGAGATGGAGATAACCGAGCTGCGGaaaat                                >  2:261537/2‑251 (MQ=255)                                                                                                                                                                                       cGTTACCGGACCGGCTACCAGAATCGGCACGCCCAACGCACCACACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGATCTGCGGATAatat                              <  1:365426/251‑1 (MQ=255)                                                                                                                                                                                          tACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCg                            >  2:14376/1‑250 (MQ=255)                                                                                                                                                                                                   ggCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGc                  <  1:29015/251‑1 (MQ=255)                                                                                                                                                                                                           gAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACg          <  1:145413/251‑1 (MQ=255)                                                                                                                                                                                                            aaTCGGCACGCCCCACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACgg         >  1:132555/1‑251 (MQ=255)                                                                                                                                                                                                                   ccGCCCATCTCACAAAACGCCACGGGCGCAATATGGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCATCCAGCAACGCAACAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGAGGATAAGATCGAACTTCCCGCTGGCAACGGTTAAttt  <  2:60319/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             ATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTT  >  NC_000913/3120546‑3121006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑