Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,269	C→T	D4N (GAT→AAT)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,269	0	C	T	100.0%	93.7 / NA	30	D4N (GAT→AAT)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/18);  total (12/18)

ACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACC  >  NC_000913/662023‑662505                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             acatCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGACTTTTATTCTg                                                                                                                                                                                                                                           >  1:86020/1‑250 (MQ=255)     cACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACa                                                                                                                                                                                                                                      <  2:25355/251‑1 (MQ=255)             gCCCCGGCCCGTTATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGTTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGCCCGAGCTGGCGGACAAGAATTGTATTCTGATACAAAACGGc                                                                                                                                                                                                                               <  2:422965/250‑1 (MQ=255)               cccGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCAtc                                                                                                                                                                                                                            >  1:197007/1‑251 (MQ=255)                                  ccTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAggaa                                                                                                                                                                                                          >  1:136803/1‑247 (MQ=255)                                           cgcgATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGAGGACAAGAATTTTATTCTGATACAAAACGGCCTCTCCGTGGAGAATGAGTAAAAGTggggg                                                                                                                                                                                                 >  1:223777/1‑250 (MQ=255)                                                        gATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCAGCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATcaca                                                                                                                                                                                   <  1:235409/251‑1 (MQ=255)                                                           cACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCg                                                                                                                                                                                >  1:121233/1‑251 (MQ=255)                                                                         ccGGCAGTAAAATGGGCGCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTAGCGGTGAATTCATGCATAGCCTGTTAGAGGGGCTCGTAAGGCTGAAGACCGAGCTGGGGGACAAGAATTGTAGTCTGATCGAAATCGTCATCTCCGGGGAGAAGGAGTGAAAGTGGGGGAAAAGTATTTCACAGCGAGGGGAGGGGAGtt                                                                                                                                                                  <  2:421045/251‑1 (MQ=255)                                                                            gCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTAcc                                                                                                                                                               <  2:133281/251‑1 (MQ=255)                                                                                        gCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCCGATTTCCTCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGCGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAgggg                                                                                                                                                                      >  2:284691/1‑232 (MQ=255)                                                                                        gCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAgggg                                                                                                                                                                      <  1:284691/232‑1 (MQ=255)                                                                                                  tCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACgg                                                                                                                                          <  2:270900/250‑1 (MQ=255)                                                                                                  tCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACgg                                                                                                                                          <  2:121233/250‑1 (MQ=255)                                                                                                      gcctgACCTTGGGTGAATAACGGATAGTGCTCGACCAGCCAGATTTCCTCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCCTAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAACGGGGGCAAATGATATCAACGCGAGGGGAGGGGAGTTCACCGACCAGGAGCCGGGTAACGgaag                                                                                                                                       >  2:38729/1‑247 (MQ=255)                                                                                                                        aCCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATg                                                                                                                    <  1:130360/250‑1 (MQ=255)                                                                                                                                                        aaGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATAc                                                                                   <  2:360219/251‑1 (MQ=255)                                                                                                                                                           ggTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTACGTGGAGAATGAGTAAAAGTGGGGGAAAAGGATATAACAGCGAGGAGAGGGGGGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGGTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAgt                                                                                >  1:266791/1‑251 (MQ=255)                                                                                                                                                            gTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAgtg                                                                               >  1:25679/1‑251 (MQ=255)                                                                                                                                                                                 gTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTAGTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAg                                                          <  2:116828/251‑1 (MQ=255)                                                                                                                                                                                   gAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGt                                                         <  2:437207/250‑1 (MQ=255)                                                                                                                                                                                             aTAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGCGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTtgat                                                >  1:361217/1‑249 (MQ=255)                                                                                                                                                                                                     ggAGATTTGATCGTAAGGCTGAAGATCGAGCTGGCGGACAAGGAGTTTATTCTGATACAAATCGGCAGCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGGCCAGTAGCCGGGTAACGGAGAAGCGAGTTACAGTACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTAGATATGATTGGCGTTGATAGTgatagat                                      <  2:104711/251‑1 (MQ=255)                                                                                                                                                                                                            ggCTCGTAAGGCTTAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAATCATGCGGACAATATCGATTTTGCCCAGTTCTTCATTCAGTGTGTCAACCTGCGCGATATGAGTGGCGTTGATAGTGATAGATACCGAg                                <  2:437756/250‑1 (MQ=255)                                                                                                                                                                                                               ccttgaGGCTGAAGACCGTGCTGGCTGACATGAATTTTATTCTGATATAAAACGGCAGCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGTGTAGAGGGGAGTTACCCGACCAGGTGCCGGGTAACGGAGATGCGAGTTACAGACCCATGCTGCCAATATTGATTTTGCCCACGTCTTCAGACAGTGTCTCAGCCTGCTCGATATGAGTTTCGTGGTTAGTTATAGATACCGAGtcgt                            <  2:29256/246‑4 (MQ=255)                                                                                                                                                                                                                   aaGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGtt                        >  2:24306/1‑251 (MQ=255)                                                                                                                                                                                                                          aaGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTGCATACAGTGTTGCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTtg                 <  1:38729/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAg                                >  2:258392/1‑222 (MQ=255)                                                                                                                                                                                                                                        ggACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAg                                <  1:258392/222‑1 (MQ=255)                                                                                                                                                                                                                                         gACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGAGAGTGATAGATACCGATTGGTAGGTGCTTTTGCTGCTTGGTTTTAcc  <  2:402043/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             ACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACC  >  NC_000913/662023‑662505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑