Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,090,968 G→A E208K (GAG→AAG)  endA → DNA‑specific endonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,090,9680GA97.9% 148.8 / NA 47E208K (GAG→AAG) endADNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (22/24);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAG  >  NC_000913/3090724‑3091200
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        
cGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCg                                                                                                                                                                                                                                    <  1:69773/251‑1 (MQ=255)
    gaggtgaATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAgc                                                                                                                                                                                                                                 <  1:340685/250‑1 (MQ=255)
     aggtgaATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCACCCCGTGCACGCGGCGCCCTTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGCCCCTCTCCCGCCAGCAAACGCAGCTGTTCAACGCATGGACCAAGATGCATCCGGTTACCGACTGGGAGTgcgcgcgc                                                                                                                                                                                                                               >  2:145390/1‑251 (MQ=255)
                   cgcgGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGGACGGACCATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGAACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACCACCTGACACTCCCTCGCCAGCAAACGCCGCTGTTCCACGCATGGAACAAGATGTATCCGGTTACCCACTGGAAGGGCGCGCGCGATGACAGCATcgc                                                                                                                                                                                                                 >  2:322270/1‑251 (MQ=255)
                    gcgGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATcgcg                                                                                                                                                                                                                <  1:160018/251‑1 (MQ=255)
                                   aCAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGGCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt                                                                                                                                                                                                 <  2:178163/251‑1 (MQ=255)
                                     aGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGCCCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCCACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATc                                                                                                                                                                                                >  1:264454/1‑250 (MQ=255)
                                      gCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCAt                                                                                                                                                                                              <  2:375196/251‑1 (MQ=255)
                                            ggAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAAccc                                                                                                                                                                                         <  2:19890/250‑1 (MQ=255)
                                                         aGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAAcgcg                                                                                                                                                                           >  1:204344/1‑251 (MQ=255)
                                                                   ggTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTGCTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAATATGTAGCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgc                                                                                                                                                                 <  1:225771/251‑1 (MQ=255)
                                                                   ggTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAAACACCAGCGCGTGCACGCGGAGACATTGCGAGCACCTACTTCTATATGCGCGACAAATACAACCTGAAACTCTCTAGCCAGCAAACGAAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTACGAGCGCGATGAACGCATCGCGAAGGTGCCGGGCAATAATAACCCGTATGTGCAAGGAGCTTGCCAggg                                                                                                                                                                 >  1:432297/1‑250 (MQ=255)
                                                                         tGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGCAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGaa                                                                                                                                                            >  2:5674/1‑250 (MQ=255)
                                                                          gctccagggaGGTCGATTTCAAAGAAAAAGCTGCCGCACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTCCTTCTATAAGCGCGCGCAATACAACCTGACACCCTCGCGCCAGCAAACGCACGTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGTGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAag                                                                                                                                                          <  1:177968/242‑1 (MQ=255)
                                                                           cgcCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAaga                                                                                                                                                         <  1:16448/251‑1 (MQ=255)
                                                                           cgcCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGAGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGTGAAaga                                                                                                                                                         <  2:365641/251‑1 (MQ=255)
                                                                                tGAAGGTCGATTTCAAAGCAAAAGCTCCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTATCGCCAGCAACCGCAGCTGTTCAACGCATGGAACCAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAACGAGCTa                                                                                                                                                     >  2:80861/1‑250 (MQ=255)
                                                                                      tCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTac                                                                                                                                               >  1:194056/1‑250 (MQ=255)
                                                                                         aTTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACt                                                                                                                                            >  1:374109/1‑250 (MQ=255)
                                                                                             cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCgg                                                                                                                                       <  1:174891/251‑1 (MQ=255)
                                                                                                              aaCCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAAcc                                                                                                                      >  1:227059/1‑251 (MQ=255)
                                                                                                                            gCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCTCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTGGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTTCCCCAcgcg                                                                                                         <  2:227059/250‑1 (MQ=255)
                                                                                                                              aCGCGGTGCCATTACGCGCGCCTACGTCTAGATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGCGCGAGCGCGATGATAGCATCGCGCAGGAGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGACAGCGCAAACCTACACTAGCGGGATGCTTTTTTTTAACCCCTACCCCaatcgta                                                                                                       >  2:313803/1‑244 (MQ=255)
                                                                                                                                  ggTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAAcc                                                                                                  >  1:99709/1‑251 (MQ=255)
                                                                                                                                  ggTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCGGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCTCGAAAGAGCTAACCTTCACGAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACatcc                                                                                                  <  2:290947/251‑4 (MQ=255)
                                                                                                                                            cgcgcACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGgag                                                                                        <  2:141411/251‑1 (MQ=255)
                                                                                                                                                     aCTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCgg                                                                               >  2:350548/1‑251 (MQ=255)
                                                                                                                                                        tCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGAt                                                                             <  1:190844/250‑1 (MQ=255)
                                                                                                                                                               gcgcgcCCAATACAACCTGCCACTCTCTCGCCAGCAAACGCAGCTGGTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTTCAACCGCGTGGGGAGACGACGCGGATTTTTAAc                                                                      <  1:439279/250‑1 (MQ=255)
                                                                                                                                                                          aCAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATTCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCTTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATcc                                                           <  1:80861/250‑1 (MQ=255)
                                                                                                                                                                           cAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTGCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATcccc                                                         <  2:239831/251‑1 (MQ=255)
                                                                                                                                                                               atGACCCTCTCTCTCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGACGGTGCAGGGCACTCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTATCCTACACTAGCGGGATTCTCTTTGCTAACCCCTACCCCACACGCACAACCACGCGGGGAGACGACGCGGATATTTAAcccacactatccaccgcc                                                     >  2:246206/2‑234 (MQ=255)
                                                                                                                                                                                  acacTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGAGTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCTCCGCAttt                                                  <  2:164795/251‑1 (MQ=255)
                                                                                                                                                                                   cacTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCAttt                                                  >  2:164012/1‑250 (MQ=255)
                                                                                                                                                                                      tctctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTatc                                               >  2:111952/1‑250 (MQ=255)
                                                                                                                                                                                          tcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCt                                          >  1:69288/1‑251 (MQ=255)
                                                                                                                                                                                           cGCCAGCAAACGCAGGTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACTCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTg                                         <  1:68252/251‑1 (MQ=255)
                                                                                                                                                                                             ccAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGCTGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGCTTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGCCGCGGATTTTTACCTATGCGTATCCCCCGCATTTATCATCCTGa                                        >  1:264727/1‑250 (MQ=255)
                                                                                                                                                                                              cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAAc                                      >  1:145036/1‑251 (MQ=255)
                                                                                                                                                                                                        aGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGTGCGCGATGAACGCATCGCGAAGGTGAAGGGCAATCATAACCCGTATGTGCAACGCGCTTGTCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTATCCCCGCCCCCACGCGTACAACCGCGTGGGGAGACGACGCGGAGTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAg                            <  2:358485/251‑1 (MQ=255)
                                                                                                                                                                                                                  cGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTcac                  <  1:358414/251‑1 (MQ=255)
                                                                                                                                                                                                                  cGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTcac                  >  1:70874/1‑251 (MQ=255)
                                                                                                                                                                                                                   gCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTcac                  >  2:317469/1‑250 (MQ=255)
                                                                                                                                                                                                                     aTGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATc               >  1:63221/1‑251 (MQ=255)
                                                                                                                                                                                                                          acaagcggTATCCGGGTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGGGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTAGGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATGCTCACATCGCGCt          <  2:312006/244‑1 (MQ=255)
                                                                                                                                                                                                                                  gaTCCGGTTACCGACTGGAAGTGAGATCGCGAGGAAAGCATCGCGAAGGGGAAGGGCAATCATAACCCGTATGGGCAACGCGCTTGCCAGGTGCGAAAGAGCTAACCTACACTAGCGGGATTCGTTGTGGTAACCCCTCCCCCCCGCGTACAACCGCGTGGGGAGACTACGCGGATTGTGAACTATGCGTAGCCCCCGCATTTATCATCCTGAACCACTGATCAGCCATTCTCACCTCGCGCTTTGCGAAg  <  2:421635/250‑1 (MQ=255)
                                                                                                                                                                                                                                   aTCCGGTTACCGACTGGAAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTCCACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTCTGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGa    >  1:361025/1‑248 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        
CGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAG  >  NC_000913/3090724‑3091200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑