Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,449	G→A	L216L (TTG→TTA)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,449	0	G	A	95.7%	144.6 / NA	47	L216L (TTG→TTA)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (22/22);  minor base C (1/1);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAA  >  NC_000913/3065208‑3065693                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     gTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGcc                                                                                                                                                                                                                                             <  1:210209/251‑1 (MQ=255)                                              tgctgcGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGcc                                                                                                                                                                                                >  2:118408/1‑251 (MQ=255)                                                    gggCGAAGAAAGTGATCATTGAACTCAATCACTATCACGAGCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAc                                                                                                                                                                                          <  2:351982/251‑1 (MQ=255)                                                    gggCGAAGAAAGTGATAATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCCTGGAAACCAACTTACCCGACCCCGGCAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAc                                                                                                                                                                                          >  1:159741/1‑251 (MQ=255)                                                      gCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGt                                                                                                                                                                                        >  1:432387/1‑251 (MQ=255)                                                         aagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGt                                                                                                                                                                                     <  1:356552/251‑1 (MQ=255)                                                          agaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGCAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGCAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                                                                                                                                                                    >  1:205515/1‑251 (MQ=255)                                                              aGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGt                                                                                                                                                                                <  1:95673/251‑1 (MQ=255)                                                                          aCTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAgg                                                                                                                                                                    <  2:219834/251‑1 (MQ=255)                                                                          aCTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAgg                                                                                                                                                                    >  1:99680/1‑251 (MQ=255)                                                                           cTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGGGTCGATCTTCCATGCAATAGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTAACGTTCTTATTGCAgg                                                                                                                                                                    >  1:231300/1‑250 (MQ=255)                                                                             cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaa                                                                                                                                                                  >  1:331044/1‑250 (MQ=255)                                                                                         cgaaccGCGCGTTGCAGAACTGGCGTAGATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGTCGTCGTGGAAAACAACTTACCCGATGTCGGGAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAGATGGCGCATGGGc                                                                                                                                                     <  2:251702/247‑1 (MQ=255)                                                                                          gATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCg                                                                                                                                                    >  2:335278/1‑251 (MQ=255)                                                                                                  cgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCAACGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTccgc                                                                                                                                            >  1:411174/1‑251 (MQ=255)                                                                                                     tGCATAACTTGCGGATAGTTTGATTCCTGGCGCGCCCCCGCGGCGCAATAGCTTGTCGATCTTCCATGCAATGGATCGCGTCGGTTCCCGCTATGTGCAAATCGATCCGAAAAAGATTGTTTCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTTCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTccgccg                                                                                                                                          <  1:254276/250‑1 (MQ=255)                                                                                                     tGCAGAACTTGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTccgccg                                                                                                                                          <  1:352947/250‑1 (MQ=255)                                                                                                        aGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAAt                                                                                                                                      >  1:150283/1‑251 (MQ=255)                                                                                                               gCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCACATGTGCCAGCAGATTGACGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTCTTCCGCCGGAATTTCTGcc                                                                                                                               >  1:266981/1‑251 (MQ=255)                                                                                                                  gATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATGCCGCCGGAATTTCTGCCGCt                                                                                                                            <  2:41249/251‑1 (MQ=255)                                                                                                                   atatTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTg                                                                                                                           >  1:272066/1‑251 (MQ=255)                                                                                                                     atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGc                                                                                                                          <  2:331044/250‑1 (MQ=255)                                                                                                                        gtgATGCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAGTGGATCGCGTCGGTAGCCGCTATGTGCAAATCGATCCGAAAAATATTGTCGCCGTCGTGGAATCCAACTTGCCCGACGCCGGTAATATGCTGGAGAAGCAAAAGCCCATGGGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAg                                                                                                                      <  1:213534/251‑1 (MQ=255)                                                                                                                          gATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTg                                                                                                                    <  2:3311/251‑1 (MQ=255)                                                                                                                           aTTCCTGGCGCGACACCGCGGCGCAATAGCGTGTCGAGCTTCCATGCAATGGATCGCGTCGGTACCCTCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTgg                                                                                                                   <  2:378009/251‑1 (MQ=255)                                                                                                                                         aCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCCACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATc                                                                                                      >  2:382485/1‑250 (MQ=255)                                                                                                                                           cgcgGCGCAATAGCGTGTCGATCTTACATGCAATGGATCGCGTCTGTATCCGCTATGGGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCaat                                                                                                   <  2:337093/251‑1 (MQ=255)                                                                                                                                                 gcAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCTTGGAAACCAACTTACCCGACGCCTGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGAGTGCCGATATCGTGGTCTCGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTg                                                                                                                           <  1:63757/221‑1 (MQ=255)                                                                                                                                                 gcAATAGCGTGACGATCTTTCATGCCTTGGATCGCGTCGGTACCCGCTATGTGCACATCGATACGACAAAGCTTGTCGCCGTCGTGGAAAACAACTTACCCGACGCCGGTAATATGCTGGATACGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCCGGAAATGGCGCATGGGCGTATTCCGACGGAATTTCTGCCGCTg                                                                                                                           >  2:63757/1‑221 (MQ=255)                                                                                                                                                    aTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGt                                                                                           >  1:304667/1‑250 (MQ=255)                                                                                                                                                              gATCTTCCATGCAATGGATCGCGTCGGAACCCGCTATGTGCAAATCGCTACGAAAAAGATTGTCGCCGTCGTGGACAACAACTTACCCGACGCCGGTAATATGCTGGATAAGAAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGACAAGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCAGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAGTGGcgcgg                                                                                 >  1:228598/1‑249 (MQ=255)                                                                                                                                                                 cTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCAGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTgg                                                                             >  2:121569/1‑251 (MQ=255)                                                                                                                                                                   tCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGTGCGTCt                                                                               <  2:70904/247‑1 (MQ=255)                                                                                                                                                                            tGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGcc                                                                                                                               <  2:32206/190‑1 (MQ=255)                                                                                                                                                                            tGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGcc                                                                                                                               >  1:32206/1‑190 (MQ=255)                                                                                                                                                                                       ggTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATtcctcc                                                       <  1:83157/251‑1 (MQ=255)                                                                                                                                                                                               cTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatg                                                >  1:104027/1‑250 (MQ=255)                                                                                                                                                                                                     gCAAATCGATCCGAAAATGATGGTCGCCGTCGGGGAAACCAACTCCCCCAACGCCGGTAAAATGCTGGAGAAGCAAAATCCCATGTGCCATCAGATTGCCGATAACGGGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCTCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTAtt                                         <  2:234674/251‑1 (MQ=255)                                                                                                                                                                                                      cAAATCGATCCGAAAAAGATTGTAGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATACTGCGGTAATGTCTCGTCTGGGGGTAAACCCGGTAATTCCTCCGTTTATGATGAATTc                                        >  2:147664/1‑251 (MQ=255)                                                                                                                                                                                                             aTCCGAATATGATTGACGCGGTCGTGGAAACCAACTTACCCGCAGCCGGTAATTTGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGCTAACGTGGTCACGTTCTTATTGCAGGAAATGGAGCATGGGCGTATTCAGCCGGAATTTCTGCCGCTGCAAAGTGGCGTgg                                                                                                              >  2:284528/1‑174 (MQ=255)                                                                                                                                                                                                             aTCCGAACAAGATTGTCGCCGTCGTGGAAACCAACTTCCCCGACGCCGGTAATATGCTGGATAAGCAAAATACCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATAGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGAAAAGTGGCGTGGGCAATATCCATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCCGGAATTTCTCCGATTATGATGGATTCGGAAGTg                                 >  2:288284/1‑251 (MQ=255)                                                                                                                                                                                                             aTCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTgg                                                                                                              <  1:284528/174‑1 (MQ=255)                                                                                                                                                                                                               ccGAAAAAGATTGTCGCCGTCGTGGAAACCACCTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCt                               <  2:300739/251‑1 (MQ=255)                                                                                                                                                                                                                 gAAAAAGCTTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTAc                             >  2:219091/1‑251 (MQ=255)                                                                                                                                                                                                                        aTTGTCGCCGTCGTGGAAACCAACTGACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATc                      <  2:138652/251‑1 (MQ=255)                                                                                                                                                                                                                                    gtGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAAGCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCGCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt          <  1:276962/251‑1 (MQ=255)                                                                                                                                                                                                                                            cAACTTACCCGACGCCGGGAAGATGCTGGATAATCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAATCCGCCGGAATTTCGGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCGACAGGAATCGGTGGTGCATGTTCTGGaaa  <  2:259899/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     GTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAA  >  NC_000913/3065208‑3065693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑