Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,050,472	G→A	L66L (CTG→TTG)	gcvT ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,050,472	0	G	A	100.0%	136.3 / NA	40	L66L (CTG→TTG)	gcvT	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/24);  total (16/24)

GCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGG  >  NC_000913/3050231‑3050709                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              gCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCg                                                                                                                                                                                                                                      >  1:407060/1‑251 (MQ=255)                  atcaCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGa                                                                                                                                                                                                                      >  1:184184/1‑250 (MQ=255)                         aCGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATcgac                                                                                                                                                                                                              <  1:138394/251‑1 (MQ=255)                                 ttCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTc                                                                                                                                                                                                       <  2:260359/250‑1 (MQ=255)                                       gCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATAtgtg                                                                                                                                                                                                <  2:126265/251‑1 (MQ=255)                                         cGAAAGGTTCAGATTGTTGGGCAAGTCAGAAGAGGTCTTTTTCGCGGGTTGCAGAGTTAACAACGCGGCGGGCGAAATCTTCAGTAACGAAGTAGACGATGGGGTAATCTATCACACCGCCAGAGGCATTCAACATCCCCGATTAAAGGTCTTTGCCGCTTGTGGTGAGATTCGCCACATCGTTCGCCAGCAGATAACACTAAAACTCCCGGGTGCGGCTGCCGCGAGGATCGACGCTGGTCGTATGTGac                                                                                                                                                                                              >  2:254335/1‑251 (MQ=255)                                          gAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGac                                                                                                                                                                                              >  1:138260/1‑250 (MQ=255)                                          gAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGac                                                                                                                                                                                              >  1:124386/1‑250 (MQ=255)                                                          tGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCgg                                                                                                                                                                              <  1:196194/250‑1 (MQ=255)                                                                    aGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTCCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGgtacgt                                                                                                                                                                   <  2:336555/251‑1 (MQ=255)                                                                     ggAGAGGTCTGTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGGAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCGTTTGGTGAGCTTCGCCACAGCGTGCGCCAGCAGATAACGCAAAAACTCCCGTGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGgtacgta                                                                                                                                                                  <  1:424622/251‑1 (MQ=255)                                                                       agagGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATTGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTAcc                                                                                                                                                                <  1:51931/251‑1 (MQ=255)                                                                               ttttcgctgtgggtggAGTTAAAAACTAGGCGGAAGAAATCGTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCACCTTCTCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACAGCGTTCGCCAGCAGATAACGCAAAAACTCCCGTGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTCCGTACCGCatgat                                                                                                                                                         <  2:336621/236‑1 (MQ=255)                                                                                     cgGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACTCCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTtcgtc                                                                                                                                                  <  2:232803/251‑1 (MQ=255)                                                                                        gTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACCTTCCGGCATCGGTCCGTACCGCATGATGTTCGTCGAt                                                                                                                                               >  2:303266/1‑251 (MQ=255)                                                                                         tGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCCTGATGTTCGTCGAtt                                                                                                                                              >  2:308057/1‑251 (MQ=255)                                                                                                        cGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGc                                                                                                                               <  2:130945/251‑1 (MQ=255)                                                                                                             gggAAGAAATCATCAGTAAATTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATACAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCTACATCTGTCGCCAGTAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACTCGTCAAACATTCCGGCAGTGGTGCGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCgg                                                                                                                          <  1:254335/250‑1 (MQ=255)                                                                                                                    aaTCTTCAGTAAAGTAGTAGACGATGAGGTCATCTCTCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCatcatc                                                                                                                   >  1:145980/1‑251 (MQ=255)                                                                                                                                       gACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAAGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCCTGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATccacc                                                                                                 >  1:258368/1‑250 (MQ=255)                                                                                                                                       aaCGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGTCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATccacca                                                                                                <  2:321309/250‑1 (MQ=255)                                                                                                                                              aGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGt                                                                                                                                                       <  1:218097/189‑1 (MQ=255)                                                                                                                                              aGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGt                                                                                                                                                       >  2:218097/1‑189 (MQ=255)                                                                                                                                                                    agGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGACGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCg                                                                   >  1:29338/1‑251 (MQ=255)                                                                                                                                                                          tCAACCTCCCCGAGTAAAGGGCTTTGCCTCTTTTGGTGCGCTTCGCCACATCGTTCGCCAGCAGATAACGCGAAAACGCCCGGGGGCGGCTGCCGCGAAGAGCGACGATGGTCATATGTGACACATCAAACAGTCCGGCATCGGTACGTACCGCATGATGTTCGTCGAGTTGCGAACCGTAATGCAGCGGCAGCATCCAGCCGTGGAAATCCTCCATGCGAGCTCCGCAAAGGGTGTGTTGTGCGTACaa                                                              <  2:209204/250‑1 (MQ=255)                                                                                                                                                                                     gAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCAGCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATGCACCATGCGAGCGCCGCAAAGCTTGTGTTGTTCGTTCAAAGGAGTCtgttg                                                  <  2:29338/251‑1 (MQ=255)                                                                                                                                                                                       gTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGAGCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTtgtg                                                <  2:145980/251‑1 (MQ=255)                                                                                                                                                                                            gggCTTTACCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATAGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGCTTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCACAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATc                                           >  1:54501/1‑251 (MQ=255)                                                                                                                                                                                             ggCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGATCGCAAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGCAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCt                                          >  2:396140/1‑251 (MQ=255)                                                                                                                                                                                                   gCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCt                                    <  2:164438/251‑1 (MQ=255)                                                                                                                                                                                                     cGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGGGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGGTGGTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCa                                  <  2:166012/251‑1 (MQ=255)                                                                                                                                                                                                         tttGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTg                               <  2:184184/250‑1 (MQ=255)                                                                                                                                                                                                           tGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGaa                             <  1:412260/250‑1 (MQ=255)                                                                                                                                                                                                               gAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGc                        <  2:124386/251‑1 (MQ=255)                                                                                                                                                                                                                   ttCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGt                                       <  2:162372/232‑1 (MQ=255)                                                                                                                                                                                                                   ttCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGt                                       >  1:162372/1‑232 (MQ=255)                                                                                                                                                                                                                       ccACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATc                                                                                                     >  1:158283/1‑166 (MQ=255)                                                                                                                                                                                                                       ccACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGAAGCATCCAGCCGTGGAAATc                                                                                                     <  2:158283/166‑1 (MQ=255)                                                                                                                                                                                                                          caTCGTTCGACCGCAGAGACCGCAAACACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACGTCAAACATTCCGGCATCGGTACGTCCCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACa              >  2:212212/1‑250 (MQ=255)                                                                                                                                                                                                                                     agcagATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGGTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATgt  <  2:150133/251‑2 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              GCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGG  >  NC_000913/3050231‑3050709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑