| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,893,647 | C→T | Q331* (CAG→TAG) | ygcN → | putative oxidoreductase with FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,893,647 | 0 | C | T | 98.2% | 191.5 / NA | 57 | Q331* (CAG→TAG) | ygcN | putative oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (33/23); minor base A (0/1); total (33/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGG > NC_000913/2893408‑2893886 | gCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACcgcg > 1:382006/1‑249 (MQ=255) aaGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCa > 1:184076/1‑250 (MQ=255) cGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGttt > 2:407763/1‑251 (MQ=255) ggCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTc > 2:315325/1‑251 (MQ=255) ggCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTc < 2:129759/251‑1 (MQ=255) gcgcCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCttt < 1:315325/251‑1 (MQ=255) ttATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATcac > 1:198767/1‑251 (MQ=255) gAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCCAAATCTGTTTCCGCTTTATCATCACAACGTAGAgcgc > 1:172169/1‑249 (MQ=255) aTCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACACCGTAGAGCGCAGcc > 2:361290/1‑251 (MQ=255) aCTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCctgc > 1:369324/1‑251 (MQ=255) aGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTgg < 1:266479/251‑1 (MQ=255) aGTAGGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTgg < 1:243727/251‑1 (MQ=255) gTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGtt < 1:87531/251‑1 (MQ=255) tGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTc > 2:302315/1‑251 (MQ=255) aTCTGGTGAAAGCAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTAc > 1:363928/1‑249 (MQ=255) tCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGc > 1:151886/1‑251 (MQ=255) gagCCAGAAGGTGGCTTGCACAGTATGCCGGAGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGAAGCGTTGCGCAGTTGCGTCCATCCCGGAATTTCCGTGCGCGGCATGGATATGACGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTCGCACCGCGCGCCGCAACAGCTGTTTCCTCTTTATCATCAAAACCAAGAGCGCAGCCTGCCGTGGGGTGTTCTACAGCgat > 2:381902/3‑248 (MQ=255) gCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCGGTGGGATGGTCTACAGCGTTAt < 1:134057/250‑1 (MQ=255) gCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTGGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTGTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCg < 2:338510/251‑1 (MQ=255) aGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCtttt < 1:302315/251‑1 (MQ=255) aTGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGc < 2:198767/250‑1 (MQ=255) aaTACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCggg > 2:132520/1‑250 (MQ=255) aTACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACCAACGCTG‑CTAAGCGCCTGCTAGCACCGAGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCgggct > 1:62625/1‑249 (MQ=255) ccGGTAACGGTTGGGTGCTGGTGGGCGAGGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTAc < 2:246816/251‑1 (MQ=255) cGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTAcc > 1:200215/1‑251 (MQ=255) ggTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTatcg > 2:64197/1‑248 (MQ=255) tAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGATTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCGACGCCCGGGATGGTACCGt > 2:384556/1‑250 (MQ=255) aaCGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTAc > 1:130148/1‑251 (MQ=255) tgctgGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCg > 1:165422/1‑251 (MQ=255) tggtgcGCGATGCGTTGCGCAGTCGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCACCGCCCGGGATGGTACCGTACGTGGCCTGCGtt > 2:397875/1‑250 (MQ=255) gtggGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTGCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCAGCGCGAGCCGCAAAATCTGTTTCCGCTGTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGGTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAAt < 1:63495/251‑1 (MQ=255) gggCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGc > 1:435496/1‑251 (MQ=255) gATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGACTGCGTTAATGCAgg > 1:351090/1‑250 (MQ=255) gATGCGTGGCGAAGTTGCGTCATTACCGGAATTTCTGTTCGCGGCATGGATAGGGCGCTGGCGGGCGCGCAGGCTGCGGCACATACGCTG‑ATAAGCGCCGGCTTGCACCGCGCGCCGCAGAATCTGTTTCCGCTTCATCATCACAACGTAGAGCGCAGCCGGCTGTGGGATGTTCTACAGCTTTATCAGCATGTTCCGGCGCTTTTGCAACGCGCGGGATGGTTCCGTACGTGGCTTGCGTTAATGCAggt < 2:431204/251‑2 (MQ=255) tGCGCAGTTGCGTCACTACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTcc > 2:261649/1‑251 (MQ=255) tGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGCACCGTACGTGGCCTGCGTTAATGCAGGATATTTc < 1:64968/250‑1 (MQ=255) aCCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATc > 1:285001/1‑250 (MQ=255) cGGAATTGCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAgg < 2:215356/251‑1 (MQ=255) gAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAgggg > 2:350712/1‑250 (MQ=255) tttCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGGTGGTACCGTACGTGGCCTGCGTTAATGCAGGCTATTTCCCGCGATTTATGGGATCAGGGTGAt > 1:215764/1‑251 (MQ=255) ttccgcgagcgGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCACAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGAt > 2:30378/9‑250 (MQ=255) ttCCGTGCGCGTCATGGATAAGACGCTGACTGTCGCGTCGGCGGCGGCACAAACGCTG‑ATAAGCGCCTTCTAGCACCGCGGGCCGCAAATTCTGTTGTCGCTTTATCATCACATCGTAGATCACAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCTTACGTGTTCTGCGTTAATTCAGCATATTTCCCGCGATTTATGGGATCATGGTGAt < 1:230234/250‑1 (MQ=255) tCCGTGCTCGGCATGGATATGGCGTTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTGAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATa < 1:391209/250‑1 (MQ=255) gTGCGCGGCATGGCTATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAAcc > 2:283444/1‑251 (MQ=255) gTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCGGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAAcc < 2:165422/251‑1 (MQ=255) cgcgGCATGGATAAGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGt > 1:99211/1‑251 (MQ=255) cATGGATAGGGCGCTGACGGGCGCGAAGGGGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTAGCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc < 2:156944/251‑1 (MQ=255) ggtgaggggtctgccTGGCGCGCAGGCGGCGGCTCAAACG‑TGTATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCTCTTTAGCATCACAACTGAGAGCGTAGCCTGCTGTGGGATGTTCTACAGCGTTATCATCATGTTCCGGCGCTTTTGCGACGTCCGCGATGGTACCGTACGTGTCCGGCGTTAATGCAGGATATTTCTCGCGATTTATGGGATCAGGGTCATAAACCTGCTCCACCg < 2:438345/236‑1 (MQ=255) caggcgcggactgggtggaagtcggcggcACAAACGCTG‑AATGGGGCGTGCACGCTCCGCGATCCGCAAAATCGGTTTCCGCTTTATCATCACAACGTAGAGCTCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCACGGGATGGTACCGTACGTGACCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAATCCTGTTCCACCGCTgc < 2:240120/228‑1 (MQ=255) cgcTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAg < 1:361290/251‑1 (MQ=255) gcTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc < 2:112821/239‑1 (MQ=255) gcTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc > 1:112821/1‑239 (MQ=255) ggCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGACTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTc < 2:72402/249‑1 (MQ=255) gcAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTTTTCCACCGCTGCGCCCGTTATTCTGGcatc > 2:28825/1‑251 (MQ=255) aCGCTG‑ATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACgg < 1:30378/251‑1 (MQ=255) aTAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgtg > 2:331756/1‑251 (MQ=255) tAAGCGCCTGCTAGCACCGCACGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGTATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATCTTTCCCGCGATTTATGGGCTCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgttg > 2:341866/1‑249 (MQ=255) | GCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTG‑ATAAGCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGG > NC_000913/2893408‑2893886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |