Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,883,115	G→A	P342S (CCT→TCT)	casA ←	type I‑E CRISPR system Cascade subunit CasA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,883,115	0	G	A	100.0%	64.1 / NA	20	P342S (CCT→TCT)	casA	type I‑E CRISPR system Cascade subunit CasA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

AGAAATGCCTTTCTGCAGTCTCATGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGGACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATA  >  NC_000913/2882869‑2883358                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aGAAATGCCTTTCTGCAGTCTCATGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACtt                                                                                                                                                                                                                                                 <  2:230215/251‑1 (MQ=255)                       aTGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAAtt                                                                                                                                                                                                                           >  1:18425/1‑251 (MQ=255)                                      ggCCCCTTTGAAGTCTCTATTTTTAAACCCTTATGCAACGGTATCTAACGCCTTGCGTCAGGCTGTTTTATATCCCAAACCCACAGTCACTATTTCGTTTCTCAAATTGCCGTATTGTTGCCACACCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCACTATTTCTGAATTGATTCACAAccgccg                                                                                                                                                                                                            >  2:209928/1‑251 (MQ=255)                                                              aaaCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACCCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCcattttcattt                                                                                                                                                                                     <  2:114315/250‑1 (MQ=255)                                                                     tCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTCCGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGaata                                                                                                                                                                               <  1:215049/249‑1 (MQ=255)                                                                        ccAAATGTATATAACTCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTGTTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCtt                                                                                                                                                                          <  2:18425/250‑1 (MQ=255)                                                                                       gCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCg                                                                                                                                                           >  2:114295/1‑251 (MQ=255)                                                                                                      gTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGTTGCTTGATTATTACGATAGCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTAAATTGATTCACAACCTCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACGCGGCTGATTTGTGTcc                                                                                                                                             <  1:388452/250‑1 (MQ=255)                                                                                                                 cAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCgg                                                                                                                                 >  2:364919/1‑251 (MQ=255)                                                                                                                  aaaCCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCggg                                                                                                                                >  1:159597/1‑250 (MQ=255)                                                                                                                          aGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGAATTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGgtggtg                                                                                                                         >  1:319626/1‑250 (MQ=255)                                                                                                                                                    cGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTcc                                                                                              >  2:52291/1‑251 (MQ=255)                                                                                                                                                             gCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCtt                                                                                      <  1:46557/250‑1 (MQ=255)                                                                                                                                                                                         atGAGGCCGTTCAAAAATAGATGCTTGATTTTTACGATATCCCCCCAGAATCAATTCAAGAGAACTTGGCGGCGCAATTTTTCTGAATTGTTTCACAACCGCCGCCACGCGATTTCCAGTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAGCCTCCCCTTTCATTATTGTTACCAGACAAGGGGAATGCGGAt                                                         <  2:50379/251‑1 (MQ=255)                                                                                                                                                                                                                    attattACGATATCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAgg                              >  1:119326/1‑251 (MQ=255)                                                                                                                                                                                                                        ttaCGATATCCCCCCATAATCACTTCACGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGCTTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACAAGACAAGGGGAATGCGGCTGGGGCCATAGCCCCTTAACTGTAAAGGTaa                           >  2:48460/1‑250 (MQ=255)                                                                                                                                                                                                                          aCGATAACCCCCCATAATCAATTCAAGAGAACTTGGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCTCGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCGCAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAATAAATGTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAAt                         <  1:438490/250‑1 (MQ=255)                                                                                                                                                                                                                           cGATACCCCCCCATAATCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATGTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTGTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAAttt                       <  1:251838/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaTCAATTCAAGAGAACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAAc         >  1:419141/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cAAGAGAACTTTGCGGAGCAATATTTCTGAATTGCTTAACAACCGCAGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAAATCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGAAAATTTTTCCTTAAGAAAACCGGtata  >  2:197592/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AGAAATGCCTTTCTGCAGTCTCATGAACAGAGACTCCGGCCCCTTTGAAGTCTTTATTTTTAAACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGCTTGATTATTACGATATCCCCCCATAATCAATTCAAGAGGACTTTGCGGCGCAATATTTCTGAATTGATTCACAACCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATA  >  NC_000913/2882869‑2883358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑