Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,881,212 C→T A311T (GCG→ACG)  casC ← type I‑E CRISPR system Cascade subunit CasC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,881,2120CT96.0% 71.7 / ‑6.2 25A311T (GCG→ACG) casCtype I‑E CRISPR system Cascade subunit CasC
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (10/14);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAGCATATGAACAACATGGGTTGCAATTTCCAGAGCCTGCTCCCTGGAGGCACCACCTAA  >  NC_000913/2880969‑2881428
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        
gTAGGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGtata                                                                                                                                                                                                                   <  1:317489/251‑1 (MQ=255)
                cAAAGGTCTGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGAGTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGAGAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAGCGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAg                                                                                                                                                                                                    <  1:178795/250‑1 (MQ=255)
                   aGGTCGGCTGCCCCCGGCCTTGCGTTGGCCCAGCAAGCCGCAAGTGAAAATAAGAGCTCATGTTCCCGCCTCGCCATTATTACGAACCCAGGACTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCGGAATTGCGCAGCAGCACCGTTCAGACCATATCCAGTGGCAACGCGATCTCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGTCAt                                                                                                                                                                                                <  2:233970/251‑1 (MQ=255)
                         gCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCg                                                                                                                                                                                          <  2:121457/251‑1 (MQ=255)
                                 ccgtttcctcttccgatcccaatccGCATGATCAAATATTTTCTCATTTTCACGCCTCGCCATTATTTCGACCCCATGTTTTTAACTGTTCTAATGTTGGCATTTTTTTAACTTGAGCAGTTATTGGGTCTCCTTCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCATACCATATCCATTGGCTACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAAcc                                                                                                                                                                                  <  1:87024/228‑1 (MQ=255)
                                                    cAAGCCGCAAGATAAAATAAGATCTCATGTACACGCCTCGCCATTATTACGAACACAGGATTTTAACTGTTCTAAAGAAGGCATTTGTTTAACTTGAGCAGCAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAAcc                                                                                                                                                                                  >  2:87024/1‑232 (MQ=255)
                                                                tCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTg                                                                                                                                                   >  2:327244/1‑251 (MQ=255)
                                                                ccAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTg                                                                                                                                                   <  2:306801/250‑1 (MQ=255)
                                                                 cAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGCTTTTAACTGTTCTAAAGTCGGCATTTGTTTAACTTGAGCAGTACTTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCACTATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTgg                                                                                                                                                  >  2:136070/1‑251 (MQ=255)
                                                                             cATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGaa                                                                                                                                      <  2:326253/251‑1 (MQ=255)
                                                                                       ccTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTATCTTGAGCAGTAATTTGGTCTACATCAGATAAGCTGAATTGCGCATCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACTGCTTTTTCAAAAGCAGGTGCCATATAAAGTGGCATATCGGAGAAATTAACCAtt                                                                                                                            <  2:296056/251‑1 (MQ=255)
                                                                                        cTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCAtt                                                                                                                            >  1:67874/1‑250 (MQ=255)
                                                                                          cGCCATTATTACGAACCCAGTATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGTGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGTAGAAATTACCCATTAc                                                                                                                          <  2:310602/250‑1 (MQ=255)
                                                                                             cATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGTTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGACATGTCGGAGAAATTAACCATTACCata                                                                                                                      <  2:155493/251‑1 (MQ=255)
                                                                                                          ccAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTaa                                                                                                         <  2:67874/251‑1 (MQ=255)
                                                                                                             ggATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAg                                                                                                      <  2:303702/251‑1 (MQ=255)
                                                                                                                ttttAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGctg                                                                                                   >  2:302597/1‑249 (MQ=255)
                                                                                                                 tttAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGc                                                                                                  >  1:256384/1‑251 (MQ=255)
                                                                                                                               aGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGtt                                                                                    >  2:208101/1‑251 (MQ=255)
                                                                                                                                                   gAGCAGTACTGGGGCCTACATATGATCAGCTGAATTGCGCCGAAGCCCCGTTCACACAATCCCCATTGGCAACGCGATCCCCCTATTGATTAACA‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATACAAAGTGGCATATCGGAGAAATTCACCATTACCATATCCGAAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGtt                                                                >  2:207437/1‑251 (MQ=255)
                                                                                                                                                    gtaagtaatatggtctgcatacgagaaGCTGAATTTCGCAGCCGCTCCGTTCTGTCCAGAAGCAATGGCAAAGCGATCCCAATTTTGATTAAACCTCGTGTA‑AGAAGGTTTCAAAAAGCCCGCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTGACCATTACCATATCCGCAGGGTTAAAATCGGCATAAGTACGCTTTTTTGCTCCAGGGACTTGTGTTg                                                               <  2:31771/226‑1 (MQ=255)
                                                                                                                                                       aGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTACAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGcc                                                             >  1:417457/1‑250 (MQ=255)
                                                                                                                                                                  cTACATCAGATAAGCTGACTTGCGCAGCAGATCCGTTCAGACCCTATCCATTGAAAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCCAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGAATTTGCCATAGAAAGAGGCTTATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGAGGCATAAGAAGGCTGTTTTGCTCACGGGCCCTCTGTTGCCAGCTTATGaac                                                  >  2:398816/1‑250 (MQ=255)
                                                                                                                                                                               gCTGAATTGCGCAGCAGCTCCGTACAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCAGCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAGCATATGAACAACATGGGTTGCaa                                    <  1:421898/251‑1 (MQ=255)
                                                                                                                                                                                                                 ccATTGGCAACGCGATCCCAATATTGATTAAAC‑GTCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATAGCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAGCATATGAACAACATGGGTTGCAATTTCCAGAGCCTGCTCCCTGGAGGCACCACCTaa  <  1:433484/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        
GTAGGTCGCGTTCCTTCAAAGGTCGGCTGCCCCCAGGCTTGCATTGGCCCAGCAAGCCGCAAGATCAAATAAGATCTCATGTTCACGCCTCGCCATTATTACGAACCCAGGATTTTAACTGTTCTAAAGTAGGCATTTGTTTAACTTGAGCAGTAATTGGGTCTACATCAGATAAGCTGAATTGCGCAGCAGCTCCGTTCAGACCATATCCATTGGCAACGCGATCCCAATATTGATTAAAC‑GCCTGTATAGACGGTTGCAAAAAGCCATCTTTCGCTTTAACCGCTTTTTCAAAAGCATTTGCCATAGAAAGTGGCATATCGGAGAAATTAACCATTACCATATCCGCAGGGTTAAAAGCGGCATAAGTACGCTGTTTTGCTCCAGGGACCTCTGTTGCCAGCATATGAACAACATGGGTTGCAATTTCCAGAGCCTGCTCCCTGGAGGCACCACCTAA  >  NC_000913/2880969‑2881428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑