Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,950,1730AG24.2% 135.8 / 10.1 67E311G (GAG→GGG) argAN‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base A (7/43);  new base G (16/0);  total (24/43)
Fisher's exact test for biased strand distribution p-value = 2.87e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCGGCTT  >  NC_000913/2949928‑2950422
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                  
agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCCGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGGGTTGTTCTCACGCGCCGGAATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCCGATTCGTCGCGAAACAATCAACGATATTGGCGGTATTTTGGC‑GTTgg                                                                                                                                                                                                                                                              >  1:434774/1‑250 (MQ=255)
               aGAAGCCCAGGAAGAGAAAGGCGATTACAACGCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAGCAGTTATCAGGAAGAGGGCGCGCTGTTGCAAGAGTTGGTCTCACGCGACGGTATCGGTACGCATATTGTGATTGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGagc                                                                                                                                                                                                                                              <  1:429190/251‑1 (MQ=255)
                         gAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGGCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTAt                                                                                                                                                                                                                                 <  2:145250/251‑1 (MQ=255)
                               aaaGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCCTTTAATCAGTTATCCGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGATTCGccccccgtagagtctggaagagcgccg                                                                                                                                                                                                                               >  2:172110/1‑227 (MQ=255)
                               aaaGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGGCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTAGCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGag                                                                                                                                                                                                                                               <  1:172110/234‑1 (MQ=255)
                               aaaGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGAGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTTGG‑GTTGATTGGCCC‑CCCAGAGCAA‑C‑AA‑GGTATTTTGGt                                                                                                                                                                                                                           >  2:287596/1‑251 (MQ=255)
                                    cGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGcc                                                                                                                                                                                                                      <  1:332075/251‑1 (MQ=255)
                                        tACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑tt                                                                                                                                                                                                                  <  1:67567/250‑1 (MQ=255)
                                         ccAACGCCGGTACTGTGCGTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTACCGGTACGCAGATTGTGATGGAAAGCGCC‑ATCATATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCGGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑Ttctc                                                                                                                                                                                                               <  1:277459/250‑1 (MQ=255)
                                            aCTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GGTGATTCGCCC‑ACTGGAGACG‑C‑AA‑GGTATTCTTGTGCGCCG‑TTCTcgc                                                                                                                                                                                                             >  2:335680/1‑251 (MQ=255)
                                                cGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GGTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGGATTCTGGAACGCCG‑TTCTCGCGagc                                                                                                                                                                                                         >  1:154285/1‑251 (MQ=255)
                                                  gTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGagcag                                                                                                                                                                                                       <  2:397549/251‑1 (MQ=255)
                                                   tACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGATTTGCCC‑ACTGGAGCAG‑C‑AA‑GGGAATCTGGTACGCCG‑TTCTCGCGggcagg                                                                                                                                                                                                      >  1:102724/1‑250 (MQ=255)
                                                      ggTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGCCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGCGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGACTCTGGG‑GTTGTTTAGCCC‑ACTGGAGCAG‑C‑AA‑GGGATTCTGGTACGCCC‑TTCTCCCGAGGAGCTgg                                                                                                                                                                                                   >  1:223457/1‑251 (MQ=255)
                                                             tttttGCGTGGCGCAGTGAACGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTTTGGG‑GTTTGTTTGCCC‑CCCGGAGCAA‑C‑CA‑GGGATTCTTGTCCGCAG‑GTCTCGCGAGCCGATGgagacggg                                                                                                                                                                                            >  2:358144/1‑247 (MQ=255)
                                                                tggCGTGTCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTAGCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCGCACGCGACGGTATCGGTACGCAGATTGTGAGTGAAAGCGCCGAGCAGATTCGTCGCGCATCAATCATCGATATTGGCGGGATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGGATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGaaa                                                                                                                                                                                          <  2:437676/248‑1 (MQ=255)
                                                                   cGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGATTCGGCC‑CCTGGGGCAG‑C‑CA‑GGGATTTTTGGACGCCG‑TTTTCGCGCGCAGCGGGGGATGGAAATCgg                                                                                                                                                                                      >  2:271599/1‑250 (MQ=255)
                                                                            gTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGTTTTGCCC‑CCTGGAGCAG‑C‑AAAGGTGTT‑TTGTACGCCG‑TTCTCGCGGGCAGCTggggagtgaaagaaccgaagaggg                                                                                                                                                                             >  2:93389/1‑229 (MQ=255)
                                                                            gTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGaa                                                                                                                                                                                           <  1:93389/237‑1 (MQ=255)
                                                                              gAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATGTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTAGCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAAGCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGGACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCa                                                                                                                                                                           <  1:41951/251‑1 (MQ=255)
                                                                               aaaGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGTGCAGATTCTTCGCGCAACAATCAACGATATTGGCGGTATGCTGGA‑GTTGATTCGCGC‑ACTGGAGCAG‑C‑AA‑GTTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAAGTCAcaat                                                                                                                                                                          <  2:434774/251‑4 (MQ=255)
                                                                                     tGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGATTTTGGG‑GGTGATTCGCCC‑CCTTGAGGAG‑C‑AA‑GGGATTCTGGGACGCCG‑TTTTCGCGGGCAGCTGGGGAGGGGAAACGACAAATTTACCcttatttc                                                                                                                                                                    >  2:254925/1‑249 (MQ=255)
                                                                                      gCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCa                                                                                                                                                                    <  2:167702/250‑1 (MQ=255)
                                                                                       ccGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTTATTTGCCC‑CCATGAGCAG‑C‑AA‑GGGATTTTGGTACGCCG‑GTCTCGAGAGCAGCTGGGGATGGAAAGTGACAAATTCACCATTATTTAgc                                                                                                                                                                  >  2:113063/1‑251 (MQ=255)
                                                                                         gCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAgcgc                                                                                                                                                                <  2:355589/251‑1 (MQ=255)
                                                                                            gcggcgTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGATCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGAt                                                                                                                                                             <  1:183970/251‑1 (MQ=255)
                                                                                            gcggcgTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAAGCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGATCAGCTGGAGATGGAAAGCGACAAATACACCATTATTCAGCGCGAt                                                                                                                                                             <  2:223457/251‑1 (MQ=255)
                                                                                                 gtgcgtCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATGCAGCGCGATAacac                                                                                                                                                        <  1:69392/251‑1 (MQ=255)
                                                                                                       cGCTGTCATTTAATCAGTGATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACGGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGATCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TAt                                                                                                                                                 <  2:250296/251‑1 (MQ=255)
                                                                                                             cATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTg                                                                                                                                           <  1:271599/251‑1 (MQ=255)
                                                                                                                   aTCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTTATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTTGCGGTATGCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCAGTATTCAGCGCGATAACACGAC‑TATTGCCTGcgccgc                                                                                                                                     <  2:211534/251‑1 (MQ=255)
                                                                                                                    tCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGGTTTGCCC‑ACTGGAGCAG‑C‑AA‑GGGATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTTACCATTATTCAGCGCGATAAAACGAC‑TATTGCCTGCGCcgcg                                                                                                                                    >  1:164041/1‑251 (MQ=255)
                                                                                                                          aTCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GGTGATTCTCCC‑ACTGGGGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTTAGCGCGATAAAACGAC‑TATTGCCTTCGCCGCGCTCTAt                                                                                                                              >  1:375420/1‑251 (MQ=255)
                                                                                                                            cAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATcc                                                                                                                            <  1:216872/251‑1 (MQ=255)
                                                                                                                            cAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATc                                                                                                                             <  1:38542/250‑1 (MQ=255)
                                                                                                                             aGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCg                                                                                                                           <  1:319060/251‑1 (MQ=255)
                                                                                                                              ggAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTTTGGG‑GTTGATTCGCCC‑ACCGGAGCAG‑C‑AA‑AGTATTCTTGGACGCCGGTTTTC‑CGAGACGCTGGGGGAGGAAATCGACAAAATCACCATTATTCAGCGCGATAACAAGAA‑TATTGGCTGCGCCGCGCTCTATccct                                                                                                                          >  2:268560/1‑249 (MQ=255)
                                                                                                                                aaGATGGCGCGCTGTTGCAAGAGTTGCTCTCCCGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTTCGCCGCGCTATATCCGTTc                                                                                                                        <  1:357826/251‑1 (MQ=255)
                                                                                                                                aaGATGGCGCGCTGTTGCAAGAGCTGTTCTCACGCGACGGTATCGGTCCGCAGATTGTGATGGAAAGCGCCGAGCAGATTCATCGCGCAACAATCACCGATATTGGAGGTATTCTGGA‑GGTGGTTTGCCCCAC‑GGAGAAGAG‑AA‑GG‑ATTCTGGTACGCCG‑GTCTGGCCGGCAGCTGGAGGTAGAGATTGACAAATTTACAATTATTTAGCGCGCTAAAACGACATATTGC‑TGcgccgccgcccttccctat                                                                                                                        >  2:357826/1‑238 (MQ=255)
                                                                                                                                 aGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGAc                                                                                                                                                                                     <  1:261913/190‑1 (MQ=255)
                                                                                                                                   aTGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCg                                                                                                                     <  2:375420/251‑1 (MQ=255)
                                                                                                                                          gcTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTTGG‑GTTGATTTCCCC‑ACAGGAG‑AGAC‑AA‑GGGAATCTTGTACGCCG‑TTCTCTCGGGCAGCTGGAGATGGAAATCGACAAAATCACCATTCTTTAGCGCGATAACACGCC‑CATTTCCTGCGCCGCGCTCTTTCCGtatccggatgga                                                                                                               >  2:95842/1‑239 (MQ=255)
                                                                                                                                          gcTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGATTCTGGG‑GTTGATTTGCCC‑CCTGGAGCAG‑C‑AA‑GGGATTTTGGTACGCCG‑TTCTCGCGAGCAGCTGGGGATGGGAATCCACAAATTCACCCTTATTCAGCGCGATAACACGAC‑TATTGCCTGGGCCGCGCTCTATCCCTTCCCGgaag                                                                                                                 >  2:185545/1‑248 (MQ=255)
                                                                                                                                             gtggCAAGAGTTGTTCTCGCGCGACTGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATAGTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAAGCGACAAAGTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCTCTCTCTATCCGTTCCCGgaagagaaga                                                                                                           <  2:21884/248‑1 (MQ=255)
                                                                                                                                             gTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGgaagagaag                                                                                                            <  2:105983/250‑1 (MQ=255)
                                                                                                                                                   agagTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGGAATTTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAAATCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGGAGAGAAGAGTgggg                                                                                                     >  1:360082/1‑251 (MQ=255)
                                                                                                                                                                 gcgACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCtgtgtg                                                                                      <  1:185545/251‑1 (MQ=255)
                                                                                                                                                                         aTCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGAATACTAGA‑GTTGAATCGCCC‑CCTGGAGCAA‑C‑AC‑GGGATTCTGGGCCACCA‑TTACCGCCGGCACCTGGAGATGGAAAAACCCAAATTCACCAATCTTCTACGCGCTAACACGAC‑TATTGCCTGCGCCGCGCACTCTCAGTTCCCGGGAGAACAGGTTGGGGGAAATGCCCtggtgggaattc                                                                                >  1:291495/1‑240 (MQ=255)
                                                                                                                                                                            ggTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑Accc                                                                          <  2:100390/251‑1 (MQ=255)
                                                                                                                                                                               aCGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCgg                                                                        <  1:358144/250‑1 (MQ=255)
                                                                                                                                                                                cGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACCGGAGCAG‑C‑AA‑GGTATTCTGGGACACCG‑TTCTCCCGAGCAGCTGGGGGTGGAAATCGACAAAATCACCATTATTTAGCGCGATAACACGAC‑TATTGCCCGCGCCCCGCCCTTGCCGGTCCCGGATGAGAAGATTGGGG‑GAATGGCCTGTGGGGGAGTTC‑Accctggt                                                                      >  2:351311/1‑247 (MQ=255)
                                                                                                                                                                                        gtgATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCa                                                               <  2:154285/250‑1 (MQ=255)
                                                                                                                                                                                        gtgATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCGGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCa                                                               <  1:220866/250‑1 (MQ=255)
                                                                                                                                                                                          gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTGCCGCAGt                                                             <  2:183095/250‑1 (MQ=255)
                                                                                                                                                                                          gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGt                                                             <  1:346471/250‑1 (MQ=255)
                                                                                                                                                                                            tGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGATTCGCCC‑ACTGGAGCAG‑CCAA‑GG‑ATTCTGGTACGCCG‑CTCTCGCCAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGCTAACAAGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCCGAAGAGAAGATTGGGG‑GAATGGCCTGTGTGGCAGGTC‑CCCCCGCTTACCGCAGTtc                                                           >  1:221445/1‑250 (MQ=255)
                                                                                                                                                                                                     ccgaagaGATTCGTCGCGCAACAAATAACGATATCGGCGGTGTTTCGGA‑GTTGATTCGCAC‑ACTGGAGCAG‑C‑AA‑GGTAGTCTGTTACGCCG‑TTCTCGCGAGACGCTGGAGATGGAAATCGACAAATTCCCCATTATTCAGCGCGAGAACACGAC‑TAGTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAAGGGCCTGTGTGGCAGTTC‑ACCCGGATGACCGCAGCTCATCTAGGGGt                                                 <  2:166209/245‑1 (MQ=255)
                                                                                                                                                                                                     ccGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAT‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGt                                                 <  2:212367/251‑1 (MQ=255)
                                                                                                                                                                                                             aTTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGG‑GTTGATTCGCCC‑CCTGGAGCAG‑C‑AA‑GGGATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATAGACAAATTCACCATTATTTCGCGCGATAACACGAC‑TATTGTCTGCGCCGCGCTCTATCCGGTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGGGGCAGTTC‑ACCCGGATTACCGAAGTTCTTAAAGGGGTggaggttt                                         >  1:28340/1‑244 (MQ=255)
                                                                                                                                                                                                             aTTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTct                                         <  1:335680/251‑1 (MQ=255)
                                                                                                                                                                                                                     cgcAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAAc                                 <  1:254925/251‑1 (MQ=255)
                                                                                                                                                                                                                      gcAACAATCAACGATATTGGAGGTATTCTGGAAGTTG‑TTCGCCC‑ACCGGGGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTTACCCTTATTCAGCGCGATAAAACAAC‑TATTTCCTGCGCCGCGCTCTCTCCGTTCCCGGGAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTCCACCCGG‑GTACCGCAGTTCATCTCGGGGGGTAGTTTTGCTgggac                                 >  1:382037/1‑247 (MQ=255)
                                                                                                                                                                                                                          caaTCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCAtt                            <  1:90921/251‑1 (MQ=255)
                                                                                                                                                                                                                                          cGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCTCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGGTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGTCGAAGc            <  2:30753/251‑1 (MQ=255)
                                                                                                                                                                                                                                          cGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCAGTGCCGCTCAGGCGAAGc            <  1:317215/251‑1 (MQ=255)
                                                                                                                                                                                                                                                tCTGGA‑GTTGATGCGCCC‑ACTGGAGCAG‑C‑AA‑GGGATTCTGTGACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATACAGCGCGATAACACGTC‑TATTGCCGGCGCCGCGCTCGATCCGTTCCCGGAAGAGAAGAGTGGGT‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGGTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGc       <  1:263626/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCg      <  1:287596/248‑1 (MQ=255)
                                                                                                                                                                                                                                                     a‑GTTGCTTCGCCC‑ACTGGAGAAG‑C‑AA‑GGTATTCTGGCACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATACCACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCGGCtt  >  2:316266/1‑250 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                  
AGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGA‑GTTGATTCGCCC‑ACTGGAGCAG‑C‑AA‑GGTATTCTGGTACGCCG‑TTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGAC‑TATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGG‑AAATGGCCTGTGTGGCAGTTC‑ACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCGGCTT  >  NC_000913/2949928‑2950422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑