Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,171,3840AC32.3% 41.5 / 26.4 31K285Q (AAG→CAG) insF5IS3 element protein InsF
Reads supporting (aligned to +/- strand):  ref base A (11/10);  new base C (4/6);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCT  >  NC_000913/2171190‑2171629
                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
cAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACCATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGGAGGATCAgagtggtgttgt                                                                                                                                                                                               >  2:81137/1‑240 (MQ=2)
cAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtggtgttga                                                                                                                                                                                               <  1:54530/251‑12 (MQ=2)
 aaaaGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGAGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGCACAATTTGAAAACCAGAAACTCGCTTAGGCCTGTGTCCATATTACGGGGGTAGGCTCAgagaggtgttgat                                                                                                                                                                                              >  1:75219/1‑239 (MQ=11)
        tgctgcTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGGGTCCATATTACGTGGGTAGGATCAgagtggtgttgatgcaggc                                                                                                                                                                                        <  2:144932/250‑19 (MQ=11)
         gctgctACGATAATGCCTGCGTGGAAAGCTGCTTTCATTCGCTGAAAGGGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtggtgttgatgcaggcc                                                                                                                                                                                       <  1:30432/250‑20 (MQ=11)
           ggctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGGGGATCAtttt                                                                                                                                                                                    <  1:268958/250‑1 (MQ=38)
            gctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtggtgttgatgcaggccagtg                                                                                                                                                                                   >  1:19009/1‑228 (MQ=11)
            gctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCAtttt                                                                                                                                                                                    <  1:12105/250‑1 (MQ=40)
             ctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTAGCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtggtg                                                                                                                                                                                                   <  1:261396/234‑8 (MQ=2)
               aCGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGAc                                                                                                                                                                                 <  2:300620/250‑1 (MQ=255)
                 gATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACACTTTGAAAACAAGAACCTCGCTTAGGCCTGTGACCATATTACGTGGGTAGGATCAGTCCTCCCTTCGTGGATCATTTTGACTgc                                                                                                                                                                              >  2:263924/1‑251 (MQ=255)
                    aaTGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTgccgc                                                                                                                                                                           >  1:97471/1‑251 (MQ=255)
                             gTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTCCGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTAt                                                                                                                                                                  >  1:298933/1‑251 (MQ=255)
                                       tcttTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGGGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCTCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGAGCATGTTGACGGCCGCCTGCGCTAGTCTGttttt                                                                                                                                                         <  2:32055/250‑1 (MQ=255)
                                                   tGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGAGCAACGGAGTTTAATTATATCGCATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGACGCCCGCGCTATTCTGTTTTTTGGCGCGCTGGCg                                                                                                                                            >  1:254939/1‑251 (MQ=255)
                                                        gTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTg                                                                                                                                       <  1:409049/251‑1 (MQ=255)
                                                                    cATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCttt                                                                                                                           >  1:325992/1‑251 (MQ=255)
                                                                                      aGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGGTCAgagtggtgttgatgcagg                                                                                                                                                                                         >  2:149461/1‑154 (MQ=11)
                                                                                      aGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtggtgttgatgcagg                                                                                                                                                                                         <  1:149461/171‑18 (MQ=11)
                                                                                       gCCGGGAAATAATGCGGGCAACGGTGTGTAATTATATCGAATGTGATTACAAGCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGtc                                                                                                        <  1:249135/251‑1 (MQ=255)
                                                                                               ataatGCGGGCAACGGTGTTTAATTATATCGAGTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCCTCCCTTCGTGGATCATTTTGACTGCAGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCGGGGTCCCGGTTTc                                                                                                >  2:37787/1‑251 (MQ=255)
                                                                                                          aaCGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtg                                                                                                                                                                                                      >  1:80480/1‑134 (MQ=2)
                                                                                                          aaCGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAgagtg                                                                                                                                                                                                      <  2:80480/138‑5 (MQ=2)
                                                                                                            cGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGTGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTACTGCCAGAc                                                                                   <  2:351173/251‑1 (MQ=255)
                                                                                                             ggTGTTTAATTATATCGAATGTGATTACAATCGGGGGCGGCGGCACAGTTGGTGTGGCGGACTCAGTCCGGAACAATGTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTTCGTGGGTAGGATCAGTCATCCCTTCGTGGATCAGTTTGACTGCCGCCTGCGCTATTCTGTTTGTTGGCGCGGGGGGGCTCTGAAATCGCTCTTTAAACCCGACTTCCTGCGGCTCAGTTGCACTATTACCAGAcc                                                                                  <  2:249891/251‑2 (MQ=18)
                                                                                                               tgtTTAATTAAATCGAATGAGATTACAATCGGTGGCGGAGGCACAGTTGGTGTGACTGACTAAGTCCGGAACAAATTGAAAACACGCACCTCGCATCGGACTGTGTACATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGGTCATTTTGAATGCCGCCTGCACCATTATGTTTTTTGGCGTGCTGGCGTGCTTGAATCGCTCTTTAAAACCGACTTCCTGGGTCACGGTTTTACTATTACCCGACatt                                                                                >  2:381061/1‑251 (MQ=255)
                                                                                                                      ttATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGTCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGtctc                                                                                                      <  2:203985/222‑1 (MQ=255)
                                                                                                                      ttATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGtctc                                                                                                      >  1:203985/1‑222 (MQ=255)
                                                                                                                                     ttACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAAAAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGGGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCTCCGCACCGCCATGa                                                           >  1:300075/1‑250 (MQ=255)
                                                                                                                                       ccagtCGGGGGCGCCGGCCCAGTTGGTGTGGCGGCCTCGGTCCGAAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCTTATTACGTGGGTAGGATCATTCTTCCCTTCGTTGATCATTTTGACTGCCGCCGCCGCATTTCTTTTTTTGGGCGCGCTGTCGAGCTGAAATCCCCCTTTAAACCCGACTTCCTGGGTTTCGTTTTCACTTTTACCAGACATTATTTTCGCCGCTCCGGCATGaaa                                                         <  2:152553/246‑1 (MQ=18)
                                                                                                                                                                                             aaaaCAAGAACCTCGATTAGGCCTGTGTCCATATTAAGTGGGTAGGATCAGTCATCCCTTCGTGGAACATTTTGACTGCCGCCTGCGCTATTATGTTTTTTGGCGCGCTGGCGAGCTGACATCGCTCTTTAAACACGACTTCCTGGGTCTCGGTTTCACTATTACCCGACATTATTTTCGCCGCACCGCCCTGAAAGCGCGTAAACACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCt  >  2:387092/1‑251 (MQ=255)
                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
CAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCT  >  NC_000913/2171190‑2171629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: