Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,343,293	+GA	coding (36/219 nt)	osmB ←	osmotically‑inducible lipoprotein OsmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,343,290	1	.	A	85.7%	53.9 / 2.7	22	coding (39/219 nt)	osmB	osmotically‑inducible lipoprotein OsmB
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (9/9);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01
*	NC_000913	1,343,290	2	.	G	86.4%	59.1 / 3.6	22	coding (39/219 nt)	osmB	osmotically‑inducible lipoprotein OsmB
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (10/9);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

AACAGAATCAAGCGTATTATAATTGCGAGTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCC‑‑A‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGTCTTAAT  >  NC_000913/1343057‑1343526                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                                 aaCAGAATCAAGCGTAATATAATTGCGAGTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCCCCTAAGGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGTTGTTGCGGGCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTc                                                                                                                                                                                                                               <  1:277074/251‑1 (MQ=255)                   ataATTGCGAGTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACa                                                                                                                                                                                                             <  2:151566/251‑1 (MQ=255)                             gTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTCATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAc                                                                                                                                                                                                   <  1:113449/251‑1 (MQ=255)                                      cGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑ATCCGCGGTCATTTTTTTGCTCGTGACAAACATAATACTCTCTCCTg                                                                                                                                                                                          <  2:294756/251‑1 (MQ=255)                                                                                         cAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCAACCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCCGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACACACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAAt                                                                                                                                        >  1:201671/1‑250 (MQ=255)                                                                                                       aatgatcacAACGTACTGCGATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTAGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTCCCAGA‑‑GA‑AC‑AGCCGCGGGCATTTTTTTGCTCGTTAAAAACATAATACTCTCTCCTGAATTTATGATTCATGGAAGTAAGCTCTCTATAACTATAATAACTATGATTAAGTCTGGTTAAatt                                                                                                                         >  2:386472/9‑251 (MQ=255)                                                                                                                aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGtt                                                                                                                                                                                                                <  1:183581/155‑1 (MQ=255)                                                                                                                aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGtt                                                                                                                                                                                                                >  2:183581/1‑155 (MQ=255)                                                                                                                    tACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACCTAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGACTAAGTCTGGTGAAATTATTCCGTGAAATc                                                                                                            >  2:433356/1‑251 (MQ=255)                                                                                                                     aCTGCCATCGGTCAGTTCTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGGTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATc                                                                                                            <  1:117773/250‑1 (MQ=255)                                                                                                                      cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTg                                                                                                          >  2:311746/1‑251 (MQ=255)                                                                                                                              ggTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGACCAG‑CACCTCAGAGACATTGCCAAAGTAATTGCC‑‑A‑‑GAGACCAGCCGCGGTCATTTTTTTGCTCGTTAAAAACATACTACTCTCTCCTGATTTTATGCTTCACGGAGGTAAGCTCTCTATAACTATAATAGCTAAGATTAAGTCTGTTGAAATTATTCCGTGAATTCTTCTcgcgcg                                                                                                  >  2:169447/1‑251 (MQ=255)                                                                                                                                                 aaTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAgg                                                                               >  1:432861/1‑251 (MQ=255)                                                                                                                                                              ccGCGCCGATTGCGGTGTTGCGGGTCCGTGTAGGCCAGTGAGGACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCC‑‑AGCGA‑AC‑AGCCGCGGTCATTTTTTTGCTCTTTACAAACATAAGACTCTCTCCTGAATGTATGATTCTCGGAAGTAAGCTCTCTATAACGATAATAGCTAAGAATTACTTGGGTGGAAGTATGGCGTGATATCGGCTCGCGCGTAGGTTGATAGCACTCAGGTGATAGTTACTTc                                                                  <  2:32583/251‑1 (MQ=255)                                                                                                                                                                gcgcCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCCGGGTTATATCACGCAGGCGATAATGACTTCCt                                                                >  2:159700/1‑251 (MQ=255)                                                                                                                                                                gcgcCGATTGCGGTGTTGCGGTCCCGTTTAAACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCa                                                                                                                                                                                                                              >  1:192659/1‑93 (MQ=255)                                                                                                                                                                  gcCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGt                                                              <  2:291492/251‑1 (MQ=255)                                                                                                                                                                                 gCGGTCCCGTTGAGACCAGTTAGAACAAGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCAGTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTGATATCACGCAGGTGATAATGACTTCCTGTTATTTCTCTGataat                                               <  2:160674/251‑1 (MQ=255)                                                                                                                                                                                            tAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTatat                                     <  2:401107/250‑1 (MQ=255)                                                                                                                                                                                                     tAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCC‑‑A‑‑GAGACCAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAACTCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAgtg                           >  1:145959/1‑251 (MQ=255)                                                                                                                                                                                                      aGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAgtgt                          >  2:58560/1‑251 (MQ=255)                                                                                                                                                                                                                               cAAAGTAATTGCCAGA‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTACTAACAGGTAAATCGTCTTAAt  >  1:289700/1‑251 (MQ=255)                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                                 AACAGAATCAAGCGTATTATAATTGCGAGTCGATAATCGACATTTATTTACGATTATTTACCGACCTGGTGACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCAC‑TCAGAGACATTGCCAAAGTAATTGCC‑‑A‑‑GA‑AC‑AGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGTCTTAAT  >  NC_000913/1343057‑1343526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑