Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	208.5 / NA	60	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (27/33);  total (27/33)

TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCT  >  NC_000913/3560209‑3560697                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   tcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                     <  1:170187/247‑1 (MQ=255)      tACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGTATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATc                                                                                                                                                                                                                                             <  1:364223/250‑1 (MQ=255)       cccATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCGGGCG‑TGGGGCCGATATCGATAAAC‑A‑GCGTCGAGCCTTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCa                                                                                                                                                                                                                                            <  1:305123/249‑1 (MQ=255)                        accgtggagttttttgGTGAAAATGCGCAATGTGTTGTGATGGAGCAGTGCGTGCGCTCCCTCTCCCGGCG‑TGGTGCCGTTATCGATAAAA‑A‑GCGTCGAGCCTTTGGTGTTTTCCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                          <  1:217944/238‑1 (MQ=255)                        aaCGTTGAGATTGTTGGTGACAATGCACAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCATGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAAGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                          >  2:40227/1‑251 (MQ=255)                        aaCGTTGAGACTGCTAGAGACAATGCACAAATTGCTGTGCTTGAGCACTGCGTGAGCTCACGCATCCGGCG‑TGGAGCCGCTATAGATAAAC‑A‑GCGTCGAGCCCTTGGGGCTTTGCTCCGCCACTTTACGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGTGATCGTGCCACGGCGTGTTCACCGACATGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATAAGGTTTTGCTCCGCCCGCt                                                                                                                                                                                                                          >  2:188137/1‑251 (MQ=255)                           gtgggggtgttgggggaaaaggtgcAAATGGCTGTGTTTGAGCTGTGCGGGTGTTACCGCTACCGGCG‑GGGTGCCGATA‑C‑ATAAACTACGCGGTGCCCCATGGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCT‑GGCGATCGTGCCACGGCGTGTTACCCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                                       <  2:334107/228‑1 (MQ=255)                               agatggtgggTGACAATGGGCAAATGGCTGTGATTGAGATGTGCGGGCGTTACCGCTGCCGGCG‑TGGTGCCGTTTGCGAAAAAC‑A‑GCGTCGAGCCTTTGGGGATTTGTCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                                   <  1:316355/243‑1 (MQ=255)                                  ttgttgGTGCCAATGCGCAAATGGCTGGGATGGAGCTGTGCGTGCGCTACCGTTTCCGTGG‑TGGTGCGGAAATCGATAAAC‑A‑GCGTCGATCCGTGGGGTATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCGT‑TGCGATCGGGCCACGGCGTGTGAACCTAACTGGAAGTCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt                                                                                                                                                                                                                 <  1:64057/250‑1 (MQ=255)                                   tgttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                               >  1:62118/1‑251 (MQ=255)                                          gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                                         >  1:130054/1‑250 (MQ=255)                                              aTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                                    >  1:207284/1‑251 (MQ=255)                                                  gcAAATTGCTTGGATGGAGCAGTGCGTGCGCTCCCGCTTCCGGCG‑TGGTGCCGATATCGTTAAAC‑A‑GCTTCGAGCCATTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                                <  1:269500/251‑1 (MQ=255)                                                   cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                               >  2:276323/1‑251 (MQ=255)                                                    aaaTTGCTGTGATTGAGCAGGGCTGGCGCTACCGCTTCCGGCG‑GGGTGCCGATATCGAAAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                              <  1:156532/251‑1 (MQ=255)                                                            gtgATTGAGCAGGGCGTGCGCTCCCGCTTCCGGCG‑GGGTGCCGATATCTAAAAAC‑A‑GCGTCGAGCCTTTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                                        <  1:369066/249‑1 (MQ=255)                                                              gtttGAGCAGGGCGTGCGCTACCGCTTCCGGGG‑TGGGGCCGATATCGATAACC‑A‑GCGTCGAGCCATTGGGGATGTGCCCCGCCCCTTTGCGGGCGTTGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                                                    <  1:398970/249‑1 (MQ=255)                                                              gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACggcg                                                                                                                                                                                    >  1:392862/1‑249 (MQ=255)                                                              gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCACCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                      >  1:398693/1‑249 (MQ=255)                                                               aTTGAGAAGTGCGAGCGCTACCGCTTACGGCG‑TGGGGCCGACATCGATAAAT‑A‑GGGTCGAGCCGTTGGGGACTTGATCCGCCACTTTGCGGGCGATGCGATCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCCACGCACCGACATGATGGCGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGAGCGCGGCCAATAGACTGCGTGCTGACgg                                                                                                                                                                                      >  2:187525/1‑248 (MQ=255)                                                                       gtgcgtgcGCTACCGCTTCCGGCGGTGG‑GCGGGACTCGATAAAC‑A‑GCGTCGTGCCATTGGGTTTTTGCCCCGCCACGTTGCGGGCGATGCGCTCTTTTTTTTCGTTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                                                                                                                                           <  1:108037/251‑1 (MQ=255)                                                                                   ccGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                                                                                                                               >  1:44121/1‑251 (MQ=255)                                                                                         cccgtcggggtgCTGATATCGAAAACC‑A‑GCGTCGGGCCTTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                             <  2:124692/240‑1 (MQ=255)                                                                                           ggCG‑TGGTGCCGATGTCGCTAAAC‑A‑GCGTAGCGCCATTGAGGCTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGAGTGGCCT‑TTCGTTCGTGCCACAGCGTGTTAACCGACCTGGAAGGCAGCGCCTCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCAGCGGGCTGCCGGAGAAATGCTCTACCAGACATTCGGTACTGa                                                                                                                                                        >  2:287593/1‑250 (MQ=255)                                                                                           cgtgggggtgCTGATACCGA‑AAAC‑AAGCGTCGACCTATTGGGGTTTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                        <  2:344206/245‑1 (MQ=255)                                                                                               ggggtgCCGATATCGATAAAC‑A‑GCGGCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                                                                                                                    <  1:77491/249‑1 (MQ=255)                                                                                                     ccGATATCGATAAAC‑A‑GCATCGAGCCTTGGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                                                                                                                              <  1:377317/251‑1 (MQ=255)                                                                                                            cGATAAAC‑G‑GGGTCGAGCCATGGGGTATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                       <  1:122169/251‑1 (MQ=255)                                                                                                                ataaacaccttggatccATTGGGTATTTGTGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                     <  1:426776/236‑1 (MQ=255)                                                                                                                  aC‑A‑GCTTCGAGCCATTGGGGTTTTGCTCCGCCTCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                                 <  1:272817/251‑1 (MQ=255)                                                                                                                       gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                              <  1:90248/251‑1 (MQ=255)                                                                                                                           ggAGCCATTGGGGATTTGCCCCGCTACTGTGCGGGCGATTCGCTCTTTTTCTGCGGGCTGGGTGGCCT‑TCCGATCGCGCCCCGGCGTGTTACCCAAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTACCATCTCTTCGGTACTGACATAACCCTGCTGTTTACCCAGTTCGataa                                                                                                                          <  1:41694/250‑1 (MQ=255)                                                                                                                                            ccgccGCCCCTTTGCGGGCGATGCGCTTTTTTTCTGGGGTCTGGGTGGCCT‑GGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                          <  2:207284/247‑1 (MQ=255)                                                                                                                                             cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                                        >  1:5087/1‑251 (MQ=255)                                                                                                                                              cccGCCATTTTGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCTT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTgtgt                                                                                                       <  2:240645/250‑1 (MQ=255)                                                                                                                                                cGCCTCTTTGCGGGCGATGCCCTTGTTTTCTGCGGTCTGGG‑GGTCTCTCCGAGCGTGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCCCACCGCCATGATGGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTGAACCAGTCCGATAATACCGTTGTGACGTTGtgttt                                                                                                     <  2:183437/251‑1 (MQ=255)                                                                                                                                                  ccactatccggccGATGCGCTCCTTTACTTCGGTGTGGGTGGCCT‑TGAGCTCGCGCCAAGGCGTGTTTACCGAAATGCAAGGCAGCGACGCACCGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGGCAGATCACTGCGGTCGCGGCGAGTAGTCTGCGGGCTGACGGAGAAATGATCTACCAGCTATTCGGTACTGACATCACCGTGCTGTTTAACCTGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                   >  2:183229/13‑251 (MQ=255)                                                                                                                                                          ggggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                           <  1:120986/250‑1 (MQ=255)                                                                                                                                                           gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                                                          >  1:75035/1‑251 (MQ=255)                                                                                                                                                               gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGGTGTGACGTTGTGTTTGTTTCCTTTATAAAt                                                                                      >  2:371181/1‑251 (MQ=255)                                                                                                                                                                atgggccagtttgcgtggtgcTGGGGGGCCT‑TGCGAACGTGCACCGGCGGTTTAACGGACGGGGAAGGCGGGGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTTTTCGGTACTGACATAACCCTGCTGTTTAACCAGGTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                                                     <  2:24786/231‑1 (MQ=255)                                                                                                                                                                           gtcgtcggtcggGGTGGCCG‑TGCGATCGTGCGTCGGCGGGTTACCCAAACTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGGTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatta                                                                           <  2:60678/246‑1 (MQ=255)                                                                                                                                                                             cttcGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                   >  1:328637/1‑224 (MQ=255)                                                                                                                                                                             cttcGGTCGGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                   <  2:328637/224‑1 (MQ=255)                                                                                                                                                                                   tCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttt                                                                  >  2:327538/1‑251 (MQ=255)                                                                                                                                                                                    cTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtttt                                                                 >  1:260582/1‑251 (MQ=255)                                                                                                                                                                                     ggggTGGCCT‑TGCGATCGTGCCACGGCGTGTGACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttgtcgttttc                                                                <  1:324960/250‑1 (MQ=255)                                                                                                                                                                                             cT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTCTTTTCGTTTTCGCGCCTTg                                                        >  2:284849/1‑251 (MQ=255)                                                                                                                                                                                             cT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                         >  1:45020/1‑250 (MQ=255)                                                                                                                                                                                                tGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                         <  2:45020/248‑1 (MQ=255)                                                                                                                                                                                                      cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGTCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc                                                <  1:40227/251‑1 (MQ=255)                                                                                                                                                                                                       gTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTAATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCTTTGAGCGAATc                                                >  1:322283/1‑250 (MQ=255)                                                                                                                                                                                                            ccGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTGCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                          <  1:276323/250‑1 (MQ=255)                                                                                                                                                                                                                  tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt                                    >  1:147655/1‑251 (MQ=255)                                                                                                                                                                                                                          gAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                                  <  2:26492/197‑1 (MQ=255)                                                                                                                                                                                                                          gAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                                  >  1:26492/1‑197 (MQ=255)                                                                                                                                                                                                                           aaCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc                           >  1:188133/1‑251 (MQ=255)                                                                                                                                                                                                                               ggAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg                       >  1:213094/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGCGCCGCACCGCCATGATGGCGCAGGATCAGGATTTGATCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc                >  1:288793/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc   <  2:123755/251‑1 (MQ=255)                                                                                                                                                                                                                                                    atgatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCt  >  1:331798/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAAC‑A‑GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCT  >  NC_000913/3560209‑3560697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑