Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,577,911	Δ1 bp	coding (467/2280 nt)	maeB ←	malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,577,911	0	A	.	100.0%	183.7 / NA	41	coding (467/2280 nt)	maeB	malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/19);  total (22/19)

GCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCC  >  NC_000913/2577665‑2578156                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGCTAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCCCCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGGTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTc                                                                                                                                                                                                                                                   >  2:386138/1‑251 (MQ=255)    ttGGTTTCCGCCATGTTTGGCTCGCGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGTCCCAGCGCTACCAGCAGGTTCATACAGGCGAGTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCTCCACGCGCAATCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCAt                                                                                                                                                                                                                                                 <  1:302739/250‑1 (MQ=255)          tCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACACGCGCCGGAAACCACCATCCTCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑GATTCATCC‑GCTcg                                                                                                                                                                                                                                         >  1:382417/1‑251 (MQ=255)           ccGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTcgc                                                                                                                                                                                                                                        >  1:151179/1‑251 (MQ=255)               cATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTGTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTTGCGCAg                                                                                                                                                                                                                                    <  2:61408/251‑1 (MQ=255)                            cGGCCCTGATAGATAACGCCGTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGTCACCCGCGCCGGAAACCACCATCCGCACGTCGTAGATGTTTTTCTCCACCACGCGCAAGTCGTTGAGGATGGCGGCAGTGTCGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCCCGCTCAGTTTCTGTTCAata                                                                                                                                                                                                                       <  1:87107/251‑1 (MQ=255)                                  tGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGaa                                                                                                                                                                                                                  >  1:340300/1‑250 (MQ=255)                                         taACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTg                                                                                                                                                                                                          >  1:431226/1‑251 (MQ=255)                                              ccTTTTGAAACGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGGTTTTCTCCACCACGCGCAAGCCGGTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAGCGTCGTGTAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCt                                                                                                                                                                                                     <  2:190007/251‑1 (MQ=255)                                                      aTCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTc                                                                                                                                                                                             >  2:52516/1‑251 (MQ=255)                                                         gCAAACCACGATGTTATGTTTTTGCAGGCCCAGCGCTACCAGCAGTTTCATACAGGCGATTGCTGCGGCCCCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTc                                                                                                                                                                                          <  2:216046/251‑1 (MQ=255)                                                                                gCAGACACAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAggttg                                                                                                                                                                   >  2:60750/1‑251 (MQ=255)                                                                                   gACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAggttggtt                                                                                                                                                                >  1:165177/1‑251 (MQ=255)                                                                                          cgcTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAgcgc                                                                                                                                                         >  1:74607/1‑251 (MQ=255)                                                                                                     ggTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACtt                                                                                                                                              <  2:431226/251‑1 (MQ=255)                                                                                                                         gcGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCg                                                                                                                          <  1:63769/251‑1 (MQ=255)                                                                                                                                ccGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGt                                                                                                                   <  1:274212/251‑1 (MQ=255)                                                                                                                                                caTCCGCCCGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCaaa                                                                                                   <  2:326854/251‑1 (MQ=255)                                                                                                                                                 aTCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGCGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAAt                                                                                                  >  1:149472/1‑251 (MQ=255)                                                                                                                                                     gCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGCTGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACAt                                                                                              >  2:194340/1‑251 (MQ=255)                                                                                                                                                        cGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCa                                                                                            >  2:104689/1‑250 (MQ=255)                                                                                                                                                         gTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAAt                                                                                          >  1:88649/1‑251 (MQ=255)                                                                                                                                                                 tGTTTTTCTCCACCACACGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACAGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCg                                                                                   >  2:375353/1‑250 (MQ=255)                                                                                                                                                                     gttCTCCACCACGCGCAAGCCGTTGAGGAGGGCGGCAGTGCTGATAACTTCCGTGCCGTGTTGATCGTCGTGGAATACCGGA‑TATTCATCC‑TCTCGCGCAGTTTCTGTTCAATATAGAAACATTTTGGCGCTTTAATGTCTTCGAGGTTGATGTCTCCGAAGGTTGGTTCGAGCGCTGCGACAACTTCAATAAATTTGTCCTGGTCGTGTTCGTCAACTTCAATGTCAAATGCTTCAATCCCGTCGAAtt                                                                               <  2:409330/249‑1 (MQ=255)                                                                                                                                                                      ttCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTGCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAAttt                                                                              <  1:386138/250‑1 (MQ=255)                                                                                                                                                                       tcccccccccccgccagtcGTTGAGGATGGCCGGAATGGTGATAAATGCCGTGCCGTGTTGATCGGCCTGGAAAACCGGA‑TATTCATCCCGCT‑GCGCAGTTTCTGGTCCATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTc                                                                             <  2:277543/232‑1 (MQ=255)                                                                                                                                                                        ctcCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAAt                                                                                                                                          <  2:178300/188‑1 (MQ=255)                                                                                                                                                                        ctcCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAAt                                                                                                                                          >  1:178300/1‑188 (MQ=255)                                                                                                                                                                          ccaccaCGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCtt                                                                           <  2:340300/249‑1 (MQ=255)                                                                                                                                                                          ccaccaCGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTaa                                                                         <  1:52516/251‑1 (MQ=255)                                                                                                                                                                                    cAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGcc                                                               >  1:243746/1‑251 (MQ=255)                                                                                                                                                                                     aaGCCGATGAGGCTGGCGGCAGTGCTGATAATTGACGTGCCGTGCTGATCGTCGTGGCATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGccc                                                              >  2:75618/1‑251 (MQ=255)                                                                                                                                                                                         cGTTGAGGATGGCGGCGGTGCTGATAATGGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TCTTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCGTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTTGTTCGAGCGCGGCGGCAACTTCAATAAATTTGTCCGGGTCGTTGTCGTCGACGTCGATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGATCGCCCTTGc                                                          <  2:58816/251‑1 (MQ=255)                                                                                                                                                                                          gTTGAGGCTGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACCTTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGcc                                                         >  1:27889/1‑251 (MQ=255)                                                                                                                                                                                            tGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTc                                                                                                                                                               >  2:286036/1‑147 (MQ=255)                                                                                                                                                                                            tGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTc                                                                                                                                                               <  1:286036/147‑1 (MQ=255)                                                                                                                                                                                              aGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATTCCGCCGAAGGTTGGTGCGAGCGCGGCGACAACTTCAATAAATGTGTCCGGGTCGAGTTCGTCAACTCCAATGTCAGATACATCAATCCCGGCGAAGTTCTTAAACAGAACGCCCTGGCCTTcc                                                     <  2:27889/251‑1 (MQ=255)                                                                                                                                                                                                      ggcAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCgg                                             >  1:273793/1‑251 (MQ=255)                                                                                                                                                                                                                         gtgccgtgcTGATCGTCGTGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTACTGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGcc                           >  1:284581/1‑250 (MQ=255)                                                                                                                                                                                                                                         cgtGGAATACCGGA‑TATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAAcccc           >  1:231871/1‑250 (MQ=255)                                                                                                                                                                                                                                                 aCCGGA‑TATTCATCC‑GCTCGCGCAGGTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTTGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAGCTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTGGCCTGCCAGCGCGCCAATGTAGCCTAACCCAAGCAccgct  <  2:129638/251‑2 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCC‑GCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCC  >  NC_000913/2577665‑2578156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑