Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,550	Δ1 bp	coding (865/1206 nt)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,550	0	C	.	100.0%	215.6 / NA	46	coding (865/1206 nt)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/18);  total (28/18)

ACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACG  >  NC_000913/2360309‑2360787                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              aCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTAAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGCTACCGGTTT‑TGAAAGCGt                                                                                                                                                                                                                                      >  2:338781/1‑250 (MQ=255)      cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGaa                                                                                                                                                                                                                                 >  1:109195/1‑250 (MQ=255)      cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCAACAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGac                                                                                                                                                                                                                                 >  1:349729/1‑249 (MQ=255)         aTTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGaaa                                                                                                                                                                                                                                >  1:403584/1‑248 (MQ=255)               cAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGc                                                                                                                                                                                                                         >  1:396762/1‑249 (MQ=255)                                 cGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCAGGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑GGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCAcc                                                                                                                                                                                                     <  2:403584/251‑1 (MQ=255)                                      gCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTgg                                                                                                                                                                                                >  2:365465/1‑251 (MQ=255)                                                 cAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTAACCGCTGGTGACCATCAtt                                                                                                                                                                                     >  1:28230/1‑251 (MQ=255)                                                  aaTCCGGGCTGGTCATCAGGAATTCGCTGACTGGCGTATTGGAGAACGTGCTAACCGCATGGTGAGAGTAAACAAACGAAACGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCAGCAATTTCCACCAGCGAGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGCATT‑TGCAAGTGAACGAAAAGATTGCAGTGTGCCATGGTGCTCACCACTAGTGAACATCATTc                                                                                                                                                                                    >  1:410650/1‑251 (MQ=255)                                                                                aTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTcgcgct                                                                                                                                                      >  2:352356/1‑249 (MQ=255)                                                                                 tggcggagtggagaagggactCACCGCATGGTGAGAGTAAACAGACGAACAGTGCGGCACCACCAGTTGCACCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGGTACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTcgcgca                                                                                                                                                      >  2:281734/21‑248 (MQ=255)                                                                                  ggCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATaa                                                                                                                                                    <  2:6775/251‑1 (MQ=255)                                                                                              aaGGTCATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGCTTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCAAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTAATActgc                                                                                                                                        >  2:426683/1‑251 (MQ=255)                                                                                                  ggATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGGGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTGTCATACTGCtgtg                                                                                                                                    <  2:157205/250‑1 (MQ=255)                                                                                                    aTCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTggc                                                                                                                                  >  1:81445/1‑251 (MQ=255)                                                                                                       aCCGCATGGTGAGCGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTggcggc                                                                                                                               >  1:295817/1‑251 (MQ=255)                                                                                                         cGCATGGTGAGAGTAAACAGACGAACCGTTCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCggcag                                                                                                                             <  1:261881/251‑1 (MQ=255)                                                                                                             gggTGAGAGTAAACAGCCGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCGCCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCAc                                                                                                                         <  2:289303/250‑1 (MQ=255)                                                                                                                  agagTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTc                                                                                                                    <  2:109195/251‑1 (MQ=255)                                                                                                                                   cGTGCGGCACCCCCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATTCCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTtat                                                                                                   <  2:388019/251‑1 (MQ=255)                                                                                                                                                      cccccTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGc                                                                                >  1:324018/1‑251 (MQ=255)                                                                                                                                                             gATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTTACCATCATGCCTACTGGCGCGTGGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTTTTCGATCCa                                                                                                              <  2:151813/214‑1 (MQ=255)                                                                                                                                                             gATTTAGCGCTTGCGGCAATTTCCACCAGCGCGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATCCTGTCGATACCGGTTT‑TGAAAGCGTACGCAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCa                                                                                                              >  1:151813/1‑214 (MQ=255)                                                                                                                                                                         gCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATTCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCTTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTc                                                             <  1:357177/251‑1 (MQ=255)                                                                                                                                                                                  tCCACCAGCGTGGCTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAACGCGTACGCAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCTATCCATTTCAGGTTATAGGGCCCGCAAGCGTGGGCCAGTTTGGTCGCATAGGTCAAGTCCTgt                                                    >  2:154587/1‑250 (MQ=255)                                                                                                                                                                                     accaGCGTGGTTAAACCACCGCAACAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTc                                                 >  1:98627/1‑251 (MQ=255)                                                                                                                                                                                         gCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATcc                                             >  1:342363/1‑251 (MQ=255)                                                                                                                                                                                          cGTGGTTCAACCACCGCACCAGCCAACATCCGGCTGCATACTGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTCTAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATcc                                             >  1:328747/1‑250 (MQ=255)                                                                                                                                                                                          cGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATcc                                             >  1:73963/1‑250 (MQ=255)                                                                                                                                                                                              gTTAAACCACCGCACCAGCCAACATCCGGCTGCATCATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGGGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTAGCGATAACTTTCATACTGCTGTGGCGGCCGGCACTCTTCGATCCATTTCAGGTTATAGTGCGCGCAAGCGTGTGCCCGTTGGGTCGCATAGTTCACGACATGACTCATCcaga                                          >  1:290224/1‑248 (MQ=255)                                                                                                                                                                                                  aaCCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGGGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGa                                    <  2:316121/251‑1 (MQ=255)                                                                                                                                                                                                   accaccGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAg                                   <  2:170295/251‑1 (MQ=255)                                                                                                                                                                                                     caccGCACCAGCCGACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTGCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCa                                 <  1:432933/251‑1 (MQ=255)                                                                                                                                                                                                     caccGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGc                                  >  1:312986/1‑250 (MQ=255)                                                                                                                                                                                                      accGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCAt                                <  1:338781/251‑1 (MQ=255)                                                                                                                                                                                                        cGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTa                              <  1:352356/251‑1 (MQ=255)                                                                                                                                                                                                           aCCATCCAACATCCGGCTGCATAATGTCGATAACGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCCAGCGTGGGACAGTTTGTTCGCCTAGTTCACGTCCTGACTCATCCAGCGGTCGAGCATTAAcc                           >  1:87600/1‑251 (MQ=255)                                                                                                                                                                                                            ccAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGTGCGCACGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTAACTCATCCAGCAGTCGAGCATTAAca                           >  1:231006/1‑249 (MQ=255)                                                                                                                                                                                                                 cAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATATCTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAAt                     <  1:55382/251‑1 (MQ=255)                                                                                                                                                                                                                   aCATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATcc                   <  2:396762/251‑1 (MQ=255)                                                                                                                                                                                                                   aCATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTGGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATcc                   <  2:342363/251‑1 (MQ=255)                                                                                                                                                                                                                            tGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCAt          >  1:148885/1‑251 (MQ=255)                                                                                                                                                                                                                               aTAATGTCCATACCAGTTT‑TGACAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGCTCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCAAGTCCTGACTCATCCAGCCGTAGAGCCTTAACCAGAAATCCTCACCGCAtttt       >  2:276868/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTc     >  2:73540/1‑250 (MQ=255)                                                                                                                                                                                                                                    gTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACg  <  2:98627/251‑1 (MQ=255)                                                                                                                                                                                                                                    gTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGACAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGCGCATTAACCAGTAATCCTCACCGCATTTTTCAcc  >  1:230238/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              ACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACG  >  NC_000913/2360309‑2360787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑