Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,808,180	(A)6→5	noncoding (59/73 nt)	rirA ←	small regulatory RNA RirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,808,180	0	A	.	100.0%	13.2 / NA	4	noncoding (59/73 nt)	rirA	small regulatory RNA RirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (2/2);  total (2/2)

GATGGTGTCCTGATAAAGCAGCATATCGATTTTTGCATCAGGATAATTCTGCTTGAGCGTACTGATGACAGGAGTAGTTAATAACATATCCCCATGATATCGCATCTTTATGACCAGGATTTTTCGAAATGGCTTTTCCACTAGCGACTCTTTT‑GT‑GTGATTGTCTGGTTAAG‑TTAAGCAGAAAAAAGCACGCTACCGCCCCAGGCTCAACAGCTACCTGAATACTGATAACGCACTTACTTTGACGTGCTAGTGTATCATTTCTTTCGCAGCAATCCGACCCGAATGATTTTTATACACAAAATATACTT  >  NC_000913/3807994‑3808304                                                                                                                                                                                              |                                                                                                                             gATGGTGTCCTGATAAAGCAGCACATCGATTTTTGCGTCAGGGTAATTTTTTTTCAGCGAACTAATGACGGGAGTAGTTAATAACATATCCCCATGAAAACGCATCTTAATGAGCAAAATTCTTCGAAATGGCTTATCCACAAGTAACTCTTTTTGTTGAGATTATCCGATTAAGGTGAAGCAGAAAAA‑GCACGTTACCGCCCCAGGCTCAACAGCTACCTGAATACTGATACCGCactgatattgacgcg                                                                <  2:359007/251‑13 (MQ=255)                              tttttGCGTCAGGGTAATTTTTTTTCAGCGCACTAATGACGGGAGTAGTTAATAACATATCCCCATGAAAACGCATCTTAATGAGCAAAATTCTTCGAAATGGCTTATCCACAAGTAACTCTTTTTGTTGAGATTATCCGATTAAGGTGAAGCAGAAAAA‑GCACGTTACCGCCCCAGGCTCAACAGCTACCTGAATACTGATACCGCACTGATATTGACGCGCTAGTGTATCACTTCTTTCGCGGCAATcc                                   >  1:172175/1‑251 (MQ=255)                                        agggtaattttttttcaGCGAACTAATGACGGGAGTAGTTAATAACATATCCCCATGAAAACGCATCTTAATGAGCAAAATTCTTCGAAATGGCTTATCCACAAGTAACTCTTTTTGTTGAGATTATCCGATTAAGGTGAAGCAGAAAAA‑GCACGTTACCGCCCCAGGCTCAACAGCTACCTGAATACTGATACCGCACTGATATTGACGCGCTAGTGTATCACTTCTTTCGCGGCAATCCGACCCGAATg                         >  1:163078/17‑251 (MQ=255)                                                                aatgaCGGGAGTAGTTAATAACATATCCCCATGAAAACGCATCTTAATGAGCAAAATTCTTCGAAATGGCTTATCCACAAGTAACTCTTTTTGTTGAGATTATCCGATTAAGGTGAAGCAGAAAAA‑GCACGTTACCGCCCCAGGCTCAACAGCTACCTGAATACTGATACCGCACTGATATTGACGCGCTAGTGTATCACTTCGTTCGCGGCAATCCGACCCGAATGATTTTtaagcacattgttgattt  <  1:276689/249‑17 (MQ=255)                                                                                                                                                                                              |                                                                                                                             GATGGTGTCCTGATAAAGCAGCATATCGATTTTTGCATCAGGATAATTCTGCTTGAGCGTACTGATGACAGGAGTAGTTAATAACATATCCCCATGATATCGCATCTTTATGACCAGGATTTTTCGAAATGGCTTTTCCACTAGCGACTCTTTT‑GT‑GTGATTGTCTGGTTAAG‑TTAAGCAGAAAAAAGCACGCTACCGCCCCAGGCTCAACAGCTACCTGAATACTGATAACGCACTTACTTTGACGTGCTAGTGTATCATTTCTTTCGCAGCAATCCGACCCGAATGATTTTTATACACAAAATATACTT  >  NC_000913/3807994‑3808304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑