Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,293,781	2 bp→AT	coding (239‑240/597 nt)	nrfG →	putative formate‑dependent nitrite reductase complex subunit NrfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,293,781	0	G	A	100.0%	140.3 / NA	43	G80D (GGC→GAT)	nrfG	putative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)
*	NC_000913	4,293,782	0	C	T	100.0%	151.6 / NA	43	G80D (GGC→GAT)	nrfG	putative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

ATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACG  >  NC_000913/4293543‑4294023                                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                                  aTGAAACAGCCCAAAATACCGGTGAAAGTGCTGACGACACTCACGGTATTGATGGTATTTCTCTGTGTCGGCAGGTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGGGTATCAGCGTCAGCGCGCTCCGCTACATCAGTTTGACAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCAAAAAACAGCGAACCGAGGACGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                        >  2:161337/1‑251 (MQ=255)     aaCAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGGACTATCTGTgg                                                                                                                                                                                                                                    >  2:136192/1‑251 (MQ=255)            cAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGAATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGACGGTACTGGATGAGTACTATCTGTGGCAAAACg                                                                                                                                                                                                                             >  2:271297/1‑251 (MQ=255)                                 gACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTgg                                                                                                                                                                                                        <  1:204464/251‑1 (MQ=255)                                   caacaCTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCAAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCACCAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAACAATTCGTTGCTGGCg                                                                                                                                                                                                      >  1:38995/1‑251 (MQ=255)                                         tCACGATATTGATGGGATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCAAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGGTAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGt                                                                                                                                                                                                >  2:65238/1‑251 (MQ=255)                                                atTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGt                                                                                                                                                                                         >  1:252559/1‑251 (MQ=255)                                                           ttCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTCCGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACgt                                                                                                                                                                              >  1:45962/1‑251 (MQ=255)                                                             ctctGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACgtgg                                                                                                                                                                            >  1:310374/1‑251 (MQ=255)                                                                       gCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCt                                                                                                                                                                  >  2:35536/1‑251 (MQ=255)                                                                       gCAGTTATCTGGTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGGATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCt                                                                                                                                                                  <  2:45962/251‑1 (MQ=255)                                                                               cTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGt                                                                                                                                                           >  1:129735/1‑250 (MQ=255)                                                                                      gCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCATACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATgcggcg                                                                                                                                                   >  1:323089/1‑251 (MQ=255)                                                                                         cAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTg                                                                                                                                                <  1:302310/251‑1 (MQ=255)                                                                                                 cAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGt                                                                                                                                        >  1:230782/1‑251 (MQ=255)                                                                                                   ggCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAAGCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGGTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGc                                                                                                                                      <  2:150993/251‑1 (MQ=255)                                                                                                      ggTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCttt                                                                                                                                   >  1:172706/1‑251 (MQ=255)                                                                                                           gTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTAc                                                                                                                              <  1:35536/251‑1 (MQ=255)                                                                                                              cGGAGTATCAGCGTCAGCGCGATCCGCTCCATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAg                                                                                                                           <  1:212170/251‑1 (MQ=255)                                                                                                              cGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAg                                                                                                                           <  2:172706/251‑1 (MQ=255)                                                                                                               ggAGTATCAGCGTCAGCGCGCTCCGCTACATCCGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGCACTGTATGCGGCGCTGGCGACGGTGCTTTATTAcccgg                                                                                                                          >  2:34132/1‑248 (MQ=255)                                                                                                                gAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACTGTGCTTTATTACCAGgc                                                                                                                         <  2:46973/251‑1 (MQ=255)                                                                                                                 aGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGgcc                                                                                                                        >  2:8320/1‑251 (MQ=255)                                                                                                                      cagcgtcagcgCGATCCGCTACATCCGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGGGCTTTATTACCCGgccagcc                                                                                                                    >  2:74348/1‑250 (MQ=255)                                                                                                                            cagcgCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCatat                                                                                                             <  1:136192/251‑1 (MQ=255)                                                                                                                                gcgATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAcc                                                                                                         <  1:8320/251‑1 (MQ=255)                                                                                                                                gcgATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAcc                                                                                                         <  1:191804/251‑1 (MQ=255)                                                                                                                                gcgAGCCGCTGCATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAcc                                                                                                         <  2:56785/251‑1 (MQ=255)                                                                                                                                     ccGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATCTGACCGccc                                                                                                     >  1:78349/1‑250 (MQ=255)                                                                                                                                              cAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGTGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCgcgc                                                                                           >  1:168166/1‑251 (MQ=255)                                                                                                                                                                        aGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTgg                                                                 <  1:190277/251‑1 (MQ=255)                                                                                                                                                                         gcgcAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                <  1:169728/251‑1 (MQ=255)                                                                                                                                                                            cAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCGGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCGGGCGACGGTGCTTTATTACCAGGCCAGCCAGGATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAg                                                             <  1:124824/251‑1 (MQ=255)                                                                                                                                                                                     gCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAAt                                                    <  1:161337/251‑1 (MQ=255)                                                                                                                                                                                       aTTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAAGGAAATCa                                                  <  2:323089/251‑1 (MQ=255)                                                                                                                                                                                           cAGGATAAAAACCTCGCTAATCCCCAAAACAGCGAACAGGGGGCGTTACTGTATGAGTACTATCTGTGGCAAAACGATTACAGCAAGACGTTGCTGACGTACCGTCAGGCGGTGCAACTACGTGGTGAGAACGCTGAACTGTATTCGGCGCTGGAGACGGTGCTTTAGTACCAGGACAGCCAGGATATGGCCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGTCAATAATGAAGTCACCGc                                              <  2:217108/251‑1 (MQ=255)                                                                                                                                                                                                         cgcTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTgg                                >  1:295695/1‑251 (MQ=255)                                                                                                                                                                                                             aaTCCACAAAACAGCGAACAGTGGGCGTTACTGTATGAGTACTATCTGTAGCAAAACGATTACAGCAATTCGTTGCTTGCGTACCGTCAGGCGTTGCAACTTCGTGGTGAGAGCGCTTAACTGTATGCGGCGCTGGCGACGATGCTTTAGTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAGTGAAATCACCGCCCTGATGCTGCTGGCTTc                            <  1:136371/251‑1 (MQ=255)                                                                                                                                                                                                             aaTCCACAAAACAGCGAACAGTGGGAGTTACTGGATGAGTACTATCTGTGGCAAAACGAGTACAGCAATGCGTAGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTAGTACCAGGCCATCCCGCATATGACCGCCCATGCTCTCGCAAGGATCGACAAAGCCCTCGCGCTGTACAGTAATGAAATCACTTCCCTGAtgctgggggctg                             <  2:302814/250‑8 (MQ=255)                                                                                                                                                                                                                   caAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGc                      >  1:243745/1‑251 (MQ=255)                                                                                                                                                                                                                     aaaCAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGt                    >  1:82299/1‑251 (MQ=255)                                                                                                                                                                                                                        cAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATTAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTa                 <  2:325862/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTAcg  >  1:71671/1‑251 (MQ=255)                                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                                  ATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACG  >  NC_000913/4293543‑4294023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑