Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,493	T→C	F19F (TTT→TTC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,493	0	T	C	100.0%	39.3 / NA	13	F19F (TTT→TTC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

CGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCA  >  NC_000913/4475268‑4475725                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         cgcCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCGCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATa                                                                                                                                                                                                                 <  1:307957/251‑1 (MQ=255)            gTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATTCCTGGCGTGtt                                                                                                                                                                                                      <  2:190493/251‑1 (MQ=255)               cGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTgg                                                                                                                                                                                                    <  1:21544/250‑1 (MQ=255)                   aaGCAGAAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAGATAACTCAAGGTTTACGGCGAGTTTGTGAAACGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTTATGCTTAGACGTTCTTATTCACTAAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTCAATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGgaatg                                                                                                                                                                                                >  1:32669/1‑250 (MQ=255)                                                             tAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGAGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATTAATGATTTTTCCCGTATTTCCGGGGGGCAGTATAACAGAAgg                                                                                                                                                      >  2:266754/1‑250 (MQ=255)                                                                               cGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                                                                   <  1:24038/251‑1 (MQ=255)                                                                                                                                                     tATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                                                             >  1:123727/1‑251 (MQ=255)                                                                                                                                                                      cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGAGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGATTATGTGGATTTTAGCACACGCTCCAACGAAGCGGGTAGAAATATGATgg                                            >  1:2347/1‑251 (MQ=255)                                                                                                                                                                                aaaaTATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGAGGGTAGAAATATGATGGCTCATATTa                                   >  1:217423/1‑250 (MQ=255)                                                                                                                                                                                       cAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTGCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTc                            <  1:266754/250‑1 (MQ=255)                                                                                                                                                                                                      cgcAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATAGTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTGACCCTGGGGTAGGGGTATTTTAGCAAACGCTCCAACGAATCGGGGAGAAATAGGAGGTCTTATATTAAGTCCTCATCTTATTCGAAAGa             <  2:200309/248‑1 (MQ=255)                                                                                                                                                                                                         aaCACATTACATTAGCCGACAAGTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGTATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGAGTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATAGTACGGCCTCATCTTATTCTAAAGATACt         <  2:333332/251‑1 (MQ=255)                                                                                                                                                                                                                 acattaGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCa  <  2:275786/250‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         CGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCA  >  NC_000913/4475268‑4475725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑