Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,900	C→T	G52R (GGA→AGA)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,900	0	C	T	100.0%	117.1 / NA	34	G52R (GGA→AGA)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

GATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAAT  >  NC_000913/4473664‑4474142                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   gatgatTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATACCAGGGCCTCGCCAAAGACGCCAATACCTGCATGTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCa                                                                                                                                                                                                                                      <  1:314884/251‑1 (MQ=255)               gcccgccTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGc                                                                                                                                                                                                                         >  1:295835/1‑250 (MQ=255)                  cgccTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCt                                                                                                                                                                                                                     <  1:256532/251‑1 (MQ=255)                   gccTTCGGGAATCTGCCGGGCGGCTTCAACAGCGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCtt                                                                                                                                                                                                                    >  2:226632/1‑251 (MQ=255)                                 gCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGAGTTTGAAAAGGCGATCAAGATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCTGCATCTTTCGACCCCGCa                                                                                                                                                                                                         <  2:69258/248‑1 (MQ=255)                                      gcggaTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCGTTCGACCCCGCATAGGTGaa                                                                                                                                                                                                 <  2:29004/251‑1 (MQ=255)                                       gggcTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGTCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCGTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGGCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGTCCCCGCATAGGTGAAt                                                                                                                                                                                                <  2:187838/250‑1 (MQ=255)                                       cggcTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAAt                                                                                                                                                                                                <  2:78224/251‑1 (MQ=255)                                                     gCATGATACGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCCTTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCCTAGGTGAATCGTACATTGGcccc                                                                                                                                                                                  >  2:235860/1‑251 (MQ=255)                                                     gCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcccc                                                                                                                                                                                  >  1:70359/1‑251 (MQ=255)                                                        ggATAAGGAGCATGAATATTGAGTTTGAAAAGGCGTTCAATATCGTCTGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                                                                                                                                                                               <  1:218185/250‑1 (MQ=255)                                                         gATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCCGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGCGAATCGTACATTGGCCCCATCg                                                                                                                                                                              >  2:106863/1‑251 (MQ=255)                                                                           tGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGAcccc                                                                                                                                                                                                            <  2:93666/203‑1 (MQ=255)                                                                           tGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGAcccc                                                                                                                                                                                                            >  1:93666/1‑203 (MQ=255)                                                                            gATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGa                                                                                                                                                           <  1:266044/251‑1 (MQ=255)                                                                             aTTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATc                                                                                                                                                                                                                      <  2:233854/191‑1 (MQ=255)                                                                             aTTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATc                                                                                                                                                                                                                      >  1:233854/1‑191 (MQ=255)                                                                             aTTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGAt                                                                                                                                                          <  2:6619/251‑1 (MQ=255)                                                                                        gCGATCAATATCGTCGGCCTTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCCCTAGTCTCTTGTGCCCGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGCAACGCCGTACGATAGCGGCACCg                                                                                                                                                >  1:61235/1‑250 (MQ=255)                                                                                                 atCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGAc                                                                                                                                      >  1:314667/1‑251 (MQ=255)                                                                                                    gtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGc                                                                                                                                   >  1:292236/1‑251 (MQ=255)                                                                                                      cgggatgtAATGCCAGGGGCGCGGCAAAGACGCCAATGCCTGCATTAGCCACCAGGAGAGCCAAGGCGGCGCTCTGACGAACGACAGCAAGGACAGCGTCTCTGTCAGCACTAGCTGTGAATACGGCTGTCGCTCTAGTCGCTTGGGCCAGGCGTGTAGCGGCAGCGTTCGGCCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTCCGATAGCGGCACTGATACCACGAGTGCCa                                                                                                                                 <  2:61235/244‑1 (MQ=255)                                                                                                                              cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGGCAGCACTATCTGTGAATACTGCTGTCGCGCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTCCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCt                                                                                                         <  1:235860/251‑1 (MQ=255)                                                                                                                                                         ccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCTAGGATGAGAACGGTCTTACCTGTAAAAGCGCCCATAAGGGCTCCTTGa                                                                              <  2:70359/251‑1 (MQ=255)                                                                                                                                                           aGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTTCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGTCTCCTTGAtt                                                                            <  2:314667/251‑1 (MQ=255)                                                                                                                                                                           cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCGGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCa                                                            <  1:116033/251‑1 (MQ=255)                                                                                                                                                                            gcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCAt                                                           <  1:92985/251‑1 (MQ=255)                                                                                                                                                                                              aaTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCGGTCGCTCTAGTCTCTTGTGCCAGGCGTTGAGCGGCATCTTTCGACCCCGCATAGTTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGTCACCGATACCACGACTGCCACCGAGGATGATAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAGTTATTATGTAACATGCATTACAAAACTGTTTTAACt                                         <  1:181396/251‑1 (MQ=255)                                                                                                                                                                                                             gTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGt                           <  2:81544/250‑1 (MQ=255)                                                                                                                                                                                                               cAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgc                                                                                                 <  2:14524/178‑1 (MQ=255)                                                                                                                                                                                                               cAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgc                                                                                                 >  1:14524/1‑178 (MQ=255)                                                                                                                                                                                                                    cTATCTGTGCATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGCGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCACAAGGTTTTGTAAt                   >  2:233817/1‑251 (MQ=255)                                                                                                                                                                                                                    cTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGCTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAAt                   >  1:152744/1‑251 (MQ=255)                                                                                                                                                                                                                                     tgTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCACCAGGTTTTGTAATGGTAACTAAAAAACAAt  >  2:233345/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   GATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAAT  >  NC_000913/4473664‑4474142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑