Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,449,586	G→A	P23S (CCG→TCG)	ppa ←	inorganic pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,449,586	0	G	A	100.0%	88.8 / NA	26	P23S (CCG→TCG)	ppa	inorganic pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/16);  total (10/16)

CGCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTAC  >  NC_000913/4449341‑4449830                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cGCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGAtct                                                                                                                                                                                                                                                 >  1:213715/1‑251 (MQ=255)        tCACCGGCTTCGTCGGTCATTGTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCaa                                                                                                                                                                                                                                              <  2:275136/247‑1 (MQ=255)         cACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATaac                                                                                                                                                                                                                                          >  2:101371/1‑250 (MQ=255)                          aTTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCgg                                                                                                                                                                                                                        <  2:67889/251‑1 (MQ=255)                            tttCAGACCGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGACACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCACCGAACAGGGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGcc                                                                                                                                                                                                                      >  2:236806/1‑250 (MQ=255)                             ttCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTTCAGCGGGTACAGAGTTGGGACCAGTACGTCAACCGGGTCAGCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTGGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTTTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGGAGATTTCTTCCGGCa                                                                                                                                                                                                                      <  2:54485/250‑1 (MQ=255)                                        aaCCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTGTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCGTCCGGCAGATCTTTACCCg                                                                                                                                                                                                          <  2:28863/251‑1 (MQ=255)                                            ggACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAgg                                                                                                                                                                                                      <  1:226428/251‑1 (MQ=255)                                                           acaGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCt                                                                                                                                                                                       >  1:275676/1‑251 (MQ=255)                                                            ccgcatccGGCTGCCGCGGGTACGGATTTGGGACCAGTACGTCAACCGGGTCTCCGTCCAGAGACAGTGTG‑TGGTTGATGTAACCGTAGTTTCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACTAACAGTGCGCCGCTCTCTTTGTCTATTTCGTATTTGATCGGATCTGCGTTATCCGAGATCTCAATAACAACGTAGATTTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTc                                                                                                                                                                                      <  2:211831/245‑1 (MQ=255)                                                                aCCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGt                                                                                                                                                                                  <  1:251756/251‑1 (MQ=255)                                                                    ggCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGt                                                                                                                                                                              <  1:271013/251‑1 (MQ=255)                                                                               gTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTaaa                                                                                                                                                                    >  1:122720/1‑250 (MQ=255)                                                                                           gACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGGTGGT‑GATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGTTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCTTTAGCCGAGATCTCAATAACAGCGTAGATTTCTTCCGGCAGATCTTTACCCGCAGGTACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCaaa                                                                                                                                                       <  1:306030/251‑1 (MQ=255)                                                                                              cAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGTCGTTGAGTAAGCTCCTGTCTGTTTCCTTTAAAAATATGTGGAAAACa                                                                                                                                                     >  2:130786/1‑250 (MQ=255)                                                                                                  aCGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGc                                                                                                                                                <  1:208733/251‑1 (MQ=255)                                                                                                                          gaCAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCg                                                                                                                                                                                                          <  2:278357/169‑1 (MQ=255)                                                                                                                          gaCAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCg                                                                                                                                                                                                          >  1:278357/1‑169 (MQ=255)                                                                                                                                                    aGTTGCATGGATAGAACATCGCGGTGTACATGAAGCGGTCAACGAACAGGGCGCCTCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATTTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTGTCGTCtt                                                                                               <  2:243176/251‑1 (MQ=255)                                                                                                                                                                       cgcgGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAg                                                                            <  2:275676/251‑1 (MQ=255)                                                                                                                                                                               aCATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTa                                                                       >  1:54479/1‑248 (MQ=255)                                                                                                                                                                                      gCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTTCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGtata                                                             <  2:37844/251‑1 (MQ=255)                                                                                                                                                                                           cAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACagag                                                        <  1:130786/251‑1 (MQ=255)                                                                                                                                                                                                                  ttGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCt                                                                                                                                                                                >  1:31784/1‑108 (MQ=255)                                                                                                                                                                                                                  ttGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCt                                                                                                                                                                                <  2:31784/108‑1 (MQ=255)                                                                                                                                                                                                                                                 tAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTAc  >  1:50202/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CGCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTG‑TGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTAC  >  NC_000913/4449341‑4449830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑