Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,423,337 C→T S164S (TCC→TCT)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,423,3370CT96.8% 226.0 / NA 63S164S (TCC→TCT) ulaEL‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/29);  minor base G (0/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTAT  >  NC_000913/4423096‑4423577
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
ggggCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAg                                                                                                                                                                                                                                         <  1:75287/250‑1 (MQ=255)
     aGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGAGTAAAGATATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGGCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGGGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGgcgcg                                                                                                                                                                                                                                    >  2:88203/1‑250 (MQ=255)
                         ggCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAGGGCGCTGGGATACGCGCACTAtctc                                                                                                                                                                                                                 >  1:180586/1‑250 (MQ=255)
                             cAGGGGCTGGAGATTATGCGTAACGCTATCCAGCTCGACCAGGATGTCGGTCTTCGCGTGATCCAGCTGGAGGGCTATGACGTTTACTATCAGGAAGCCAATAACGACACGCGTCGTCGTTTCCGTGACGGCCTGAAAGCGCGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGGTGGAGATCATGGATTATCCGTTGATGAGCTCTATCGGCAAGGCGCTGGGATACGCGCCCTATCTcaccc                                                                                                                                                                                                             >  2:42659/1‑247 (MQ=255)
                              aGGGGCTGGAGATTTTGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGAGGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAAt                                                                                                                                                                                                           <  2:267179/251‑1 (MQ=255)
                                       agaTTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTc                                                                                                                                                                                                  >  1:267664/1‑251 (MQ=255)
                                               cGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGATGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGAATATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCCGctct                                                                                                                                                                                           >  2:179650/1‑250 (MQ=255)
                                                      cTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGat                                                                                                                                                                                   >  2:41332/1‑251 (MQ=255)
                                                          ccAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCg                                                                                                                                                                               >  1:179502/1‑251 (MQ=255)
                                                            aGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCCGGAAGCCAATAACGAACCGCGTCGTCGTTTCCGTGACGGCCTGCAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGc                                                                                                                                                                             >  2:329675/1‑251 (MQ=255)
                                                                cGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAAcc                                                                                                                                                                         >  1:4809/1‑251 (MQ=255)
                                                                    cAGGAAGTCGGTATTCGAGTGATCCAGCTGGCGGGCTCTGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGACAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGCTGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTc                                                                                                                                                                     >  2:308189/1‑251 (MQ=255)
                                                                     aGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCg                                                                                                                                                                    <  1:238396/251‑1 (MQ=255)
                                                                       gATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGc                                                                                                                                                                  <  1:41332/251‑1 (MQ=255)
                                                                                aTTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGGCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACaa                                                                                                                                                         <  1:121304/251‑1 (MQ=255)
                                                                                               cTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGAtgtcgatcgga                                                                                                                                           >  2:261455/1‑242 (MQ=255)
                                                                                               cTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGAtgt                                                                                                                                                   <  1:261455/242‑1 (MQ=255)
                                                                                                 ggCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGa                                                                                                                                          <  1:199081/249‑1 (MQ=255)
                                                                                                  gCGGGCTATGACGTTTACTAGCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGtt                                                                                                                                       <  1:314385/251‑1 (MQ=255)
                                                                                                     ggCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACACCGATGTGCAGATGGAGTTGc                                                                                                                                     >  1:6812/1‑250 (MQ=255)
                                                                                                          tGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGCCAACGATGTGCAGATGGAGTTGCAGGCCg                                                                                                                               >  2:106420/1‑251 (MQ=255)
                                                                                                                 tACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCACCAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCgg                                                                                                                        >  1:293480/1‑251 (MQ=255)
                                                                                                                      tCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGGGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTg                                                                                                                                      >  1:307954/1‑232 (MQ=255)
                                                                                                                      tCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCCCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAggtg                                                                                                                                      <  2:307954/232‑4 (MQ=255)
                                                                                                                        aGGAAGCCACTAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGCGAGCGCTGAGATGGCAAGCCGCGCGCAGGTGACGATTGCGATGGCGATCATGGATTATCCGTTGATGAGAGCTATCTGCAAGGCGCTGGGATACGCGCACTATCTCAACAAGCCGTGGTTCCCGCTCAACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGCAGTGCAGATGGAGTTGCAGGACGGAATCGGGCATAtc                                                                                                                 >  2:118959/1‑251 (MQ=255)
                                                                                                                         ggAAGCCAATAACGAAACGCGGCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCGACCCGGATAGCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcg                                                                                                                <  1:23395/251‑1 (MQ=255)
                                                                                                                          gAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt                                                                                                               <  1:47096/251‑1 (MQ=255)
                                                                                                                          gAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt                                                                                                               >  1:206971/1‑251 (MQ=255)
                                                                                                                               cAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGCTGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCgg                                                                                                          >  2:150458/1‑251 (MQ=255)
                                                                                                                                  tAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGCTGGCAAGCCGCGCGCAGGTGACGCTGGCGTTGGAGAACATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTagcgttcc                                                                                                       >  1:296767/1‑247 (MQ=255)
                                                                                                                                   aaCGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTAc                                                                                                       <  1:240022/250‑1 (MQ=255)
                                                                                                                                       aaaCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAtgtg                                                                                                  <  1:214155/251‑1 (MQ=255)
                                                                                                                                       aaaCGCGTCGTCGTTTCCGGGACGGCCTGAAAGAGAGCGGTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCAGGGATTATCCGTTGATGAGCTCGATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAtgtg                                                                                                  <  2:34256/251‑1 (MQ=255)
                                                                                                                                        aaCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAtgtg                                                                                                  >  1:100724/1‑250 (MQ=255)
                                                                                                                                         aCGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaa                                                                                                <  1:187736/251‑1 (MQ=255)
                                                                                                                                               cgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGCGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGacac                                                                                          >  2:143971/1‑251 (MQ=255)
                                                                                                                                                gtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGAGGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCTGAATCGGGCATATCTGCGCGGTACATGTGAAAGACAcc                                                                                         <  2:216530/251‑1 (MQ=255)
                                                                                                                                                        cGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTgg                                                                                 <  1:58930/251‑1 (MQ=255)
                                                                                                                                                           gACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGt                                                                              <  1:140004/251‑1 (MQ=255)
                                                                                                                                                                ccTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTc                                                                          >  1:331681/1‑250 (MQ=255)
                                                                                                                                                                ccTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTc                                                                          >  2:80694/1‑250 (MQ=255)
                                                                                                                                                                   gAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATTCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACCCCAAACCTGGCGTCTTCaaa                                                                       <  2:1228/250‑1 (MQ=255)
                                                                                                                                                                             gTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGtt                                                            >  1:53583/1‑251 (MQ=255)
                                                                                                                                                                               ggagaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGGCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTTCATGTAAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTg                                                          <  1:126695/250‑1 (MQ=255)
                                                                                                                                                                                 agaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGc                                                        <  1:262808/251‑1 (MQ=255)
                                                                                                                                                                                  gaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCg                                                       <  2:179502/251‑1 (MQ=255)
                                                                                                                                                                                            cgcgcgCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGTCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTGCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGt                                             <  1:211138/251‑1 (MQ=255)
                                                                                                                                                                                                 gcAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCCGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAACAACGTGCCGTTTGGCGAAGGTGTAGTGGAtt                                        >  1:222192/1‑251 (MQ=255)
                                                                                                                                                                                                 gcAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGAtt                                        >  2:111362/1‑251 (MQ=255)
                                                                                                                                                                                                  cAGGTGACGCTGGCGATGGAGATCATGGATTATCCGGTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAAGCCGGGGTTCCAGCTCTAGCCGGATAGCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGTTACATGTGAAAGTCTCCAAGCCTGGCGGCTTCAAAAACGTGCCGTTTGGCGAAGGTGTatgggatt                                        <  2:296767/250‑8 (MQ=255)
                                                                                                                                                                                                       gACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCACAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCATTTGGCGAAGTTGTAGTGGATTTCGAAc                                  <  2:331681/251‑1 (MQ=255)
                                                                                                                                                                                                        aCGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg                                 >  1:231826/1‑251 (MQ=255)
                                                                                                                                                                                                        aCGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCGTGAGCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTTGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg                                 <  1:16136/251‑1 (MQ=255)
                                                                                                                                                                                                         cGCTGGCGATGGAGATCATGGATTATCCGTTTATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTGCAAAAACGTTCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgg                                <  2:24507/251‑2 (MQ=255)
                                                                                                                                                                                                             ggCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgttgt                             <  2:237522/250‑1 (MQ=255)
                                                                                                                                                                                                              gCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGCTTTCGAACGTTGttt                           >  1:10125/1‑251 (MQ=255)
                                                                                                                                                                                                                gATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCg                         <  2:196291/251‑1 (MQ=255)
                                                                                                                                                                                                                   ggAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGaaa                      <  1:25696/251‑1 (MQ=255)
                                                                                                                                                                                                                      gaTCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGc                   >  1:80731/1‑251 (MQ=255)
                                                                                                                                                                                                                            ggATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAAc             >  1:197667/1‑251 (MQ=255)
                                                                                                                                                                                                                            ggATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAAc             >  1:325891/1‑251 (MQ=255)
                                                                                                                                                                                                                                       tgatgaGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTa   <  1:106420/250‑1 (MQ=255)
                                                                                                                                                                                                                                       tgatgaGCTCTATCAGCAAGGCGATGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGACTCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGGGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTAt  >  2:194519/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
TGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTAT  >  NC_000913/4423096‑4423577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑