Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	93.3 / NA	32	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/19);  total (13/19)

CGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTG  >  NC_000913/4223397‑4223878                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  cGCCAACCTTACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGGCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTg                                                                                                                                                                                                                                           <  2:184008/250‑1 (MQ=255)    cAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                       <  1:68617/251‑1 (MQ=255)           aCGCACGAAACCCTGCTGTTTCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑aaa                                                                                                                                                                                                                               <  1:204321/251‑1 (MQ=255)               aCGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAAGTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACCCTGCCCTTGGATTGGGCCATCCACTCCGGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGATGGTGCGGTGCCAAAGGCGGGTTTTCTGACCCGTTATTCTGGGAA‑GGGGGCGACCATGGCAGTGTCCCTTTTCTGTATTGTggtacatat                                                                                                                                                                                                                            >  1:333500/1‑244 (MQ=255)                cGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGTT‑TCGCGGGGATGGGGGCGGCCATGACAGTCTCCTTTTTCTTTATTGTGG‑GAAtcatt                                                                                                                                                                                                                          >  1:101711/1‑251 (MQ=255)                cGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGTT‑TCCCGGGAATGGGGGCGACCCTGACAGTCTCCTTTTTCTGTTTCGTGG‑aaaacctt                                                                                                                                                                                                                          >  1:314583/1‑246 (MQ=255)                         gctgctGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTATCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCTGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAGCGTGT‑AAAgcattttcattt                                                                                                                                                                                                                   <  2:123594/249‑1 (MQ=255)                         gctgTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATcattttcatttt                                                                                                                                                                                                                  <  1:148302/250‑1 (MQ=255)                               gCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGt                                                                                                                                                                                                            <  2:271600/250‑1 (MQ=255)                                                    gggTCGTGGAATTGGGTAACCCGGCTTGGTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAGTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGGGCGGTGGCAACGGCGGGTTTTCTGCCGTGTT‑TCGCGGGAATTGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga                                                                                                                                                                                      <  2:138829/251‑1 (MQ=255)                                                            gAATTGGGGTACCCGGCGTGTTGCTCCAGTTCCGTGAGGGCCACACTGGCTTTGGATTCGGCAAGCCACTCCTGTAATTTCAGGCCACGCGTTATAGTCTGAACCTGTTCAGGCGCGTTTGCTGGGGCAGCGGCGGGTGTTGTGGCGCGTT‑TCGCGTGGATGGGTTCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCGAATGCAATAGTTACTGAACTGATcc                                                                                                                                                                              <  1:313509/251‑1 (MQ=255)                                                              aTTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGGT‑TTGCGGGAATGGGGGCGGGCATGACAGACTCCTTTTTTTGTATCGTGGGAAAT‑ATTTTCATTTTTTTTGTTAGGTAATGCAATAGGTACTGAACTGAttcg                                                                                                                                                                             >  1:47912/1‑247 (MQ=255)                                                              aTTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                                                                                                                                                                            <  1:326003/251‑1 (MQ=255)                                                                  ggTAACCCGGCTTGATGCGCCAGCTCCGTGAGTGCCACACGGCCATCGGAGTCGGCAATCCACTCCATTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCAGGCGCTGGTGCGGTGGCAACGGCGGTTTGTCTTCCGCGTT‑GCGCGGGAATGGGTGCGGCCAGGACAGTCTCCCTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAGTTCAGTAGTTACTTAACTGAGCCTATGAg                                                                                                                                                                        <  2:323439/251‑1 (MQ=255)                                                                                                                tGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCCTT‑TCGCGGGGATGGGGGCGACCCTGACAGTCTCCTTTTTTTGTATCGTGG‑AAATCCTTTTTATTTTTTTTTTTAGCTAATGCCATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTTCcac                                                                                                                          >  1:162373/1‑251 (MQ=255)                                                                                                                      cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATg                                                                                                                     <  2:101711/250‑1 (MQ=255)                                                                                                                          aaTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGttt                                                                                                                <  1:247722/251‑1 (MQ=255)                                                                                                                             ccccTCCAGTAATTTCAGGGCACGCGTGAAAGACTTAACCTGGCCAGTCGCTGTGGCGGTGGCGACGGCGGGTTTTCTGCCGCGTT‑TCGCGGTAATGGGTGCGACCATGACCGTCTCCTTTTTCTGTATCGTAT‑AATTCATTTTCATTTTGATTGTTAGCTAATTTAATAGTTACTGAACTGATCCGATGAGTTAATGTTGATCAAATCTCTTGTTGCGTGGTGGTCGCTTTTACcacatttggtgatacg                                                                                                             <  2:37698/248‑13 (MQ=255)                                                                                                                                           tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCGCCGTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTttg                                                                                                <  1:191312/250‑1 (MQ=255)                                                                                                                                              ggCCACGCGTTAAAGTCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGa                                                                                            <  1:235155/251‑1 (MQ=255)                                                                                                                                                               tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGTT‑TCGCGGGGATGGGGGGGAACATGACAGGCTCCTTTTTTTGTATCGTGG‑GAATCATTTTTCTTTTTTTTGTTAGCTAATGCAATAGTTTCTGAACTGATACGATGAGTTAATGTTGAACAAATTTTATGTTGCGTGGTGGGCGCTTTTTCCACAGATGCGTTTATGCCCGTTTGGTTTTTTGGATTTTTTTTAAATATgg                                                                           >  1:50833/1‑251 (MQ=255)                                                                                                                                                                gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGGATGGGTGCGGCCCTGCCAGTCTCCTTTTTTTGTATCGTTG‑AAATCATTTTCCTTTTTTTTTTTTGCTAATGCAATAATTTCTTAACCTATCCGATGAGTTTATTTTGTACCAATCTCATGTTTCGTGGTGGTCGCTTTTACCACAGATGCGTTTTTTCCCGTATGGTTTGTTGTAttgctcggcagagcccc                                                                          >  1:137900/1‑236 (MQ=255)                                                                                                                                                                gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTGTACCACAGATGCGTTGATGCCAGTATGGTTTGTTGAAt                                                                                          <  2:137900/235‑1 (MQ=255)                                                                                                                                                                                          gCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCTTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGTGCAATTGTGag                                                <  2:47912/251‑1 (MQ=255)                                                                                                                                                                                                  gggTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAg                                        <  1:76600/251‑1 (MQ=255)                                                                                                                                                                                                  gggTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTACTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCaa                                          >  1:208185/1‑249 (MQ=255)                                                                                                                                                                                                               cgTT‑TCGCGGGACTGGGAGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGACCTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcg                           >  2:234629/1‑251 (MQ=255)                                                                                                                                                                                                                           aaTGGGAGAGACCATGACAGTCTACTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGTTGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTa                 >  2:207672/1‑250 (MQ=255)                                                                                                                                                                                                                              gggTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGtt                                                                                                                 >  1:10713/1‑151 (MQ=255)                                                                                                                                                                                                                              gggTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGtt                                                                                                                 <  2:10713/151‑1 (MQ=255)                                                                                                                                                                                                                              gggTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATg             >  1:97294/1‑251 (MQ=255)                                                                                                                                                                                                                                         tGACAGTCTCCTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg  >  2:82036/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  CGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTT‑TCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGG‑AAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTG  >  NC_000913/4223397‑4223878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑