Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	90.3 / NA	27	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/16);  total (11/16)

CAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAG  >  NC_000913/3329507‑3329988                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      cAAAGCCTGTGGATATGGCTTTTATACTCTTGGCTTCTTATTCCCCCGTTACGAGGTTCAGTCAGGTACGCACCCTCCCCCGGGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCTACCGGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGTCGTGCAGGATGGAGCCAGCTATGCCGGAGCAAGTCTGAAATAGGAGTTAAAACAg                                                                                                                                                                                                                                         <  2:220405/251‑1 (MQ=255)          gTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCTTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGACGGTGCAATTCTGAAATATGAGTTAACACAGGCGGGTaat                                                                                                                                                                                                                                >  1:180469/1‑249 (MQ=255)              tatGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCt                                                                                                                                                                                                                            >  2:195464/1‑251 (MQ=255)                        ataCGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGAac                                                                                                                                                                                                                  >  1:102127/1‑251 (MQ=255)                          aCGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAACGGAacac                                                                                                                                                                                                                >  1:174690/1‑251 (MQ=255)                                        attaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACt                                                                                                                                                                                                  <  1:137046/251‑1 (MQ=255)                                           aCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTCTCACCTGGAGCGGGACACTGCTGCGCCAAACTc                                                                                                                                                                                                 >  2:139696/1‑249 (MQ=255)                                               cgtgaCGAGGTTCAGCCAGGTACGCCCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGACCCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTa                                                                                                                                                                                           <  2:160537/247‑1 (MQ=255)                                                       tGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTg                                                                                                                                                                                                                           <  1:227639/211‑1 (MQ=255)                                                            aGCCAGGTCCGCCCCCTCCCCCGTTTTTCTGCCGAAGCGCAATACTTCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAAc                                                                                                                                                                              <  1:186467/251‑1 (MQ=255)                                                                       cACCCTCCCCCGTAATTCTGCCGAAGCGCAGTACTGCGAACTGGACGTGGGGCCCCGCGACCTGAACCGCTTTAAGCTGACGGGAAGCCTCCCACAACGTTCTGAGCCGCTCCCGTTGGCTCTTGCCGTGCAGGCTGGAGCCAGCTATGCCGGTGAAATTCTGACATATGAGTTAAAACAGGCGGGTATCCACTGGGGCGGAACACAGCTGGGCCCGAAGCAGGTTTACGatactgggacgggcgttgccc                                                                                                                                                                   >  2:51558/1‑231 (MQ=255)                                                                                 ggTGGTTCTGCCGAAGCGCAATTCTGCGAACTGGATGGGGTGCCAGGCGACCGGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTGTGCCGTGCAGGATGGAGCTAGCTATGCCGGTGCAATTCTGAAATATGGGTTAAAACAGGCTGTTATCACCTGGAGCGGAACACTGCTGCGCCGGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTc                                                                                                                                                         <  1:51336/250‑1 (MQ=255)                                                                                   gggTTCTGCCGAAGCGCAATACTGCTAACTGGATGTGGGGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCGGGAACGGTAGTTGCCAGTAAACAGTCgg                                                                                                                                                       <  2:314147/250‑1 (MQ=255)                                                                                       tCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGTGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGccc                                                                                                                                                    <  2:232710/250‑1 (MQ=255)                                                                                                 cacAATACTGCGAACTGTATGGGGTTCCAGTCGCCCTGAACCGCTTTACTCGGACGGGAGGCCTGCCCCAACTTTCTGAGCCGCTCCCGTTGGCGTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACCCTTCTGCGCCAGACGCAGGTTAACGAACCTGGAACTTTAGTTGCCATTAAATAGTCGGCCCCGCTGCACGAt                                                                                                                                         <  2:193621/249‑1 (MQ=255)                                                                                                       aCTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGAGGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCt                                                                                                                                    <  1:273906/250‑1 (MQ=255)                                                                                                            gAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCGGAAAACTGCTGCGCCAGACTCAGGGTGACGAACCCGGAACGGGCGTTGCCAGTAAACAGGCCGCCCCCCTGCACCATCTGCTTa                                                                                                                                  >  2:60344/1‑247 (MQ=255)                                                                                                                                  aCCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAACTATGAGTTAAAACAGGCGGGTATCACCCGGGGCGGCACACCGCTGCGCCCGACTCAGGTTAACGAAACAGGAACGGGAGTTGCCAGTAAACAGTCCGCCCCGCTGCACGATGTGCTTAAGATTTTGCTGAAAAAGACGGacaa                                                                                                         >  2:304670/1‑250 (MQ=255)                                                                                                                                                 ggCTGGCGGGAGGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACg                                                                                         <  2:180317/250‑1 (MQ=255)                                                                                                                                                             gccggccACAACGTTCTGAGCTGCTCCCTTTGGCTTTTGCCGTGCAGGATGGAGCCAGCGATGCCGGTGCAATGCTGAAATATGATTTAAAACAGGCGGTTATCACCCGGAGCGGAACACTGCTGCGACAGCCTCAGGTTAACGATCCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGTTCTGCTTAAGAGTATGATGAAAAAGTCGGACAACAGTATCGCCGATACGGTTTTCCGCAt                                                                              <  2:62433/246‑1 (MQ=255)                                                                                                                                                                   cccaACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGc                                                                       <  2:4807/249‑1 (MQ=255)                                                                                                                                                                                gCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGGCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCa                                                          <  2:102127/251‑1 (MQ=255)                                                                                                                                                                                gCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAAAACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGCAAAAAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCCTGATAGGCCATGCGCGCTTc                                                           >  1:331273/1‑250 (MQ=255)                                                                                                                                                                                                          aGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCggg                                <  2:116612/251‑1 (MQ=255)                                                                                                                                                                                                             aTGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCg                             <  1:195464/251‑1 (MQ=255)                                                                                                                                                                                                                  gCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTACAACAGGCGGGGATCACCTGGGGCGGGACACTGCTGAGCCAGAATCAGGTTAACGAACATGGAACGGTAGTTGCCCGTAAACAGTCGGCCCCGCTGCACGGTATGCTTAAGATTATGGTGGAAAAGTCGGGCAAAATGATCGCCGATACGGTTTTACGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGCACATGgcggggcggggggggacg                        >  2:61381/1‑243 (MQ=255)                                                                                                                                                                                                                                        tGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGGCGGACGCCGTGCGGCAGGTTCTGCGcccg  >  1:76071/1‑249 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      CAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAG  >  NC_000913/3329507‑3329988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑