Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,159	A→C	C303G (TGT→GGT)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,159	0	A	C	100.0%	77.2 / NA	26	C303G (TGT→GGT)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/13);  total (13/13)

AAAATAGTTGGAGCGAGGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACG  >  NC_000913/3265921‑3266394                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            aaaaTAGTTGGAGCGAGGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATaa                                                                                                                                                                                                                                   <  2:231268/249‑1 (MQ=255)                  gCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTATTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCt                                                                                                                                                                                                                 >  2:310980/1‑250 (MQ=255)                                  aaTTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCg                                                                                                                                                                                                 >  1:72813/1‑250 (MQ=255)                                          cagccagTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTGATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCGTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATTCTGCtt                                                                                                                                                                                        <  2:148120/251‑1 (MQ=255)                                                       tttCGCTTCAATAATGTCATCAATAGCAACCGGCAGCTCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCACGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGAt                                                                                                                                                                            >  2:202909/1‑250 (MQ=255)                                                               cAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTc                                                                                                                                                                    <  1:329921/250‑1 (MQ=255)                                                                                 cAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATAtt                                                                                                                                                 >  1:333958/1‑251 (MQ=255)                                                                                         aGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCcac                                                                                                                                          >  1:29228/1‑250 (MQ=255)                                                                                           aTCGTATGTCATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACAGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTACATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCAATTATAAGATGAATACTCTTTGGCTACTTCCACCAAAACCGAAGCTGCTTTTTTAATACGCTAATTTTTCGACCATACCGCGGCATATTGTGCCACAgg                                                                                                                                       >  2:179734/1‑251 (MQ=255)                                                                                                       atGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAAGACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTttctt                                                                                                                            <  2:333958/250‑1 (MQ=255)                                                                                                        tGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTttcttc                                                                                                                           >  2:166827/1‑250 (MQ=255)                                                                                                         gCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCa                                                                                                                          <  1:166827/250‑1 (MQ=255)                                                                                                                aaaaCCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAAt                                                                                                                  <  1:310980/251‑1 (MQ=255)                                                                                                                            ccTGAATTAAATATGTATGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATGATAAGATGAATACTCTTGGGCTAATTCCACCAAAACCTAGTCTGTTTTGTTAATACTGTAATTTTTCGACCATACCGCGGCATATTGGGCCGCAGTTAATATTCCTTGAACCGGAATAGTAATAAATTg                                                                                                      <  2:330363/251‑1 (MQ=255)                                                                                                                            ccTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCTGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTg                                                                                                      <  2:44849/251‑1 (MQ=255)                                                                                                                                           tAGGTAACCGTAACCGACTCCTGCAAGACCGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCACATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGATGCttt                                                                                                                                                                                       >  2:234270/1‑155 (MQ=255)                                                                                                                                           tAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCttt                                                                                                                                                                                       <  1:234270/155‑1 (MQ=255)                                                                                                                                                                           cagGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCAGTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAAGTACAGTTAAGa                                                       <  2:29228/251‑1 (MQ=255)                                                                                                                                                                                 gATTATTTATGGTCAAACAAAAACAAATAACTAACCAACGTCTATTAATTGCCCTCGTTTTGCCCCATTATAAGATGAAGACTCTTGCGCTAACGCCGCCAAACCCGATGCTGCCTTGTTAATACGATAATTTTGCGGGCATGCCGCTCCAGATTGTGCCTCAGATAATGTTTCTTCACGCGGAATAGTAATAAATTGGTTAGAACCAAAAGTTGACGTGATATCACAAGGAATTACAGTTAAGAAATCAg                                                 <  1:14903/251‑1 (MQ=255)                                                                                                                                                                                            tttaactcaacaccaaTACCTAACCACCTTCTATTACCAGCCTTAGTCTAACCCCATTTTAAGATGAATACTATTTGACTAATTCTACCAAAACCGATACTGCTTTTTTAATACGATAATTTTTCGCCCATACCGCGGCATATTGTACCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGCCGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAAc                                       >  2:157048/15‑250 (MQ=255)                                                                                                                                                                                              taaataacAACAACTAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATCCTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACa                                      >  2:58814/1‑249 (MQ=255)                                                                                                                                                                                                aataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACa                                      >  2:289781/1‑247 (MQ=255)                                                                                                                                                                                                    aaaaCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCACAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGAAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATtat                               >  2:230937/1‑250 (MQ=255)                                                                                                                                                                                                                taacCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGAc                  >  1:88815/1‑251 (MQ=255)                                                                                                                                                                                                                              tAATTGCCTTCTGCTTCCCCCATTATAAGATTAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTTATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGTATTGAGAACAAGAGTATAAATTGTCTCGACTGAGTCGGTTTTa     <  2:11053/250‑1 (MQ=255)                                                                                                                                                                                                                                aTTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACg  <  2:277298/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            AAAATAGTTGGAGCGAGGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACG  >  NC_000913/3265921‑3266394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑