Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,076,643	C→A	E272* (GAG→TAG)	yggP ←	putative zinc‑binding dehydrogenase YggP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,076,643	0	C	A	100.0%	111.2 / NA	32	E272* (GAG→TAG)	yggP	putative zinc‑binding dehydrogenase YggP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/16);  total (16/16)

ACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATC  >  NC_000913/3076399‑3076880                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              gctgTTTCTGGCACCGCATCCAGGCCGCCGATAGGTGTCACCATAACCGACGGCTTTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATTTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGCCGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTGCCGCCAGTAATGCAGCAGCCATCTAAACGAc                                                                                                                                                                                                                                         <  1:283059/248‑1 (MQ=255)       tCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTCGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAgg                                                                                                                                                                                                                                   >  2:327744/1‑251 (MQ=255)                            ccGATATAGGTCACCATAAACGAAGGCTGTCACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGCCGTTGTAGCAATTAGACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCGAAGAAGTTCCGACAGCCATCTTCCGCTAGTATTTCATCAGCCATCTAAACGACAGCAGGCACAGCCGCATACACaaaac                                                                                                                                                                                                               >  2:1190/1‑249 (MQ=255)                                     gTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGACGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGa                                                                                                                                                                                                      >  2:231051/1‑250 (MQ=255)                                                       tGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTc                                                                                                                                                                                     >  2:56354/1‑249 (MQ=255)                                                        gtacttgccacgTGGCGCTAAGGGCAATCCCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACTTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACGGCCATCTTCCGCCAGTAATGCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAgc                                                                                                                                                                                  <  2:134477/240‑1 (MQ=255)                                                        gTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGCAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGCCATCGAACCCGGCATCTCCTGTCAgc                                                                                                                                                                                  >  2:68957/1‑251 (MQ=255)                                                                   gTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCAt                                                                                                                                                                       <  1:327744/251‑1 (MQ=255)                                                                                cAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCg                                                                                                                                                          >  1:91017/1‑251 (MQ=255)                                                                                             cATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTg                                                                                                                                              <  1:278026/250‑1 (MQ=255)                                                                                                gtcgtcCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTAt                                                                                                                                          >  2:120353/1‑251 (MQ=255)                                                                                                         tGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGATGTAGCAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCCGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAACCAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATacc                                                                                                                                 >  1:201768/1‑250 (MQ=255)                                                                                                            cccGCCAGATGTACCGACGACGTGCGTGCTGTTGTAAGGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCa                                                                                                                              <  2:91017/250‑1 (MQ=255)                                                                                                                  aGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATCCACCAGCTCa                                                                                                                         >  1:172371/1‑250 (MQ=255)                                                                                                                          gggcgacgTGCGTGCTGTTGTCATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTATCTCCGTCGGCCAGGCAAAGAAGGCCAGACAGCCATCTTCCGCCGATAATTCTTCAGCCATCTAGACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCATCAGCTGGATAGGATCGCTCGTCCCTTGGTTAGTCATCTATACCAGCTCaagcgcttta                                                                                                                <  2:140548/248‑9 (MQ=255)                                                                                                                             cgacgTGCGGGCTGTTGTAAGGGACGTTGTAGAAATTAAACGGCACTTGTAATTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTg                                                                                                             <  2:255580/251‑1 (MQ=255)                                                                                                                                          gttgtAATGGACGTTGTAGAAATTAAACGGCCCTTTGAATTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACAGACACCAGCTCAATGCCTTTACTGGCCGCCAGTTcc                                                                                                 <  2:73617/250‑1 (MQ=255)                                                                                                                                                        tGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTc                                                                                  >  2:330009/1‑251 (MQ=255)                                                                                                                                                        tGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTc                                                                                  >  1:180427/1‑251 (MQ=255)                                                                                                                                                         gTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCt                                                                                 <  1:120353/251‑1 (MQ=255)                                                                                                                                                              aaTTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCCGTTCCACCGGCAGCAGCTTCTGTAcc                                                                            >  2:279066/1‑251 (MQ=255)                                                                                                                                                              aaTTAAACGGCACGTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCATACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGTACAGGATCGGTCATTCCTTTGGTATTCACATACACGATGTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTAcc                                                                            <  1:165476/251‑1 (MQ=255)                                                                                                                                                                                  tttttATCCGTCGTCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTgtcgtc                                                        >  2:263678/1‑251 (MQ=255)                                                                                                                                                                                           gTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGac                                               <  1:330009/251‑1 (MQ=255)                                                                                                                                                                                                    gCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccaccaccac                                      <  1:312376/251‑1 (MQ=255)                                                                                                                                                                                                           agtacAGACAGCTATCTGCCGCCAGTAATTAATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATTCCTTTACTGGCCGTCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCTGACGTTTGTCTTCGAGATCGACCACCTCCACCCGCGAc                               <  2:145182/247‑1 (MQ=255)                                                                                                                                                                                                                ggACAGCCGTCTTCCGCCAGTAATTCAACAGCCATCGAAACGACAGCAGGCACCGCCGCAGAAGCAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATTCACCAGCTCAGTGCCTTTACTGGCCGCCAGTTCCACCGTCAGTAGCTTCTGTACCTGCGCCGTACGTTTGACGGGTAGATCGacccccaccaccgcctacggttg                          <  2:216507/250‑12 (MQ=255)                                                                                                                                                                                                                  aCAGCCATCTTCCGCCAGTAAATCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTa                        <  1:56354/251‑1 (MQ=255)                                                                                                                                                                                                                                ccAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAACAATGTCATCGAACACGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCATTTACTGGCCGCCAGTTCCACCGGAAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGCCGGTTGTATGCCGCCGTTAATg          >  1:193655/1‑251 (MQ=255)                                                                                                                                                                                                                                 cAGTAATTCATCAGCCATCTAAACGACCGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGACATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACCCCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATgg         >  2:288748/1‑251 (MQ=255)                                                                                                                                                                                                                                 cAGTAATTCATCAGCCATCTAAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATgg         >  2:92140/1‑251 (MQ=255)                                                                                                                                                                                                                                         catcaGCCATCTAACCGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATc  <  1:231051/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATC  >  NC_000913/3076399‑3076880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑