Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,112,577	G→A	A162V (GCG→GTG)	rfbB ←	dTDP‑glucose 4,6‑dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,112,577	0	G	A	100.0%	35.7 / NA	12	A162V (GCG→GTG)	rfbB	dTDP‑glucose 4,6‑dehydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

CAACATACAACCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTGGATGCTTTGGATGCGGAATAAGGGCTGCTTGGCGCGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGC  >  NC_000913/2112353‑2112821                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     cAACATACAACCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTGGATGCTTTGGATGCGGAATAAGGGCTGCTTGGCACGTAAGCTGTCGTCTCAGTAAATAAg                                                                                                                                                                                                                            <  1:125560/251‑1 (MQ=255)       aCAACCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTGGATGCTGTGGATGCGGAATAAGGGCTGCTTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTaa                                                                                                                                                                                                                       <  2:229288/250‑1 (MQ=255)          aCCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCCGTGCATTAAGAATCACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGCTCGCTGGATGCTTTGGATGCGGAATAAGGGCTGCTTGGCACGTAAGCTGTCGTCTCCGTAAATAAGGGTCAttct                                                                                                                                                                                                                   >  1:14550/1‑251 (MQ=255)                   ggATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCAGTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGAACATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCTGTGGATGCTTTGGATGCGGAATAAGTGCTGCTTGTCACTTAAGCTGTCTTCTCAGTGAATTAGGGTAACTCTCCTGtatta                                                                                                                                                                                                          <  2:147537/251‑1 (MQ=255)                         gatctCCTTTGCCATAAATCGGTAATGCCTTACCGTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGTGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGAGCGCTGGATGCTTTGGATGCGGAATAAGGGCTGCTTTGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTAttt                                                                                                                                                                                                       <  1:120895/248‑1 (MQ=255)                                  tGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCGCAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTTTGTGCTTTGGATGCGGAATAAGGGCTGCTTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTgg                                                                                                                                                                                            <  1:334297/250‑1 (MQ=255)                                               tAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTGGATGCTTTGGATGCGGAATACGGGCTGCTTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCAc                                                                                                                                                                              >  2:22905/1‑251 (MQ=255)                                                                                      aaTAAGCTTTTCAGGGAACTGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTGGACGCTTTGGATGCGGAATAAGGGCTGCTTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGc                                                                                                                                       >  1:249833/1‑251 (MQ=255)                                                                                                                                                                                                gATGCTTTGGATGCGGAATAAGGGCTGCTTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACGTCAGCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCACCAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAAt                             <  1:330287/251‑1 (MQ=255)                                                                                                                                                                                                                       gcagtttGGCACGTAAGCTGTCGTCTCAGTAAATAATGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTATAAATATGATGAAATCGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGGCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTcag      <  2:241391/246‑1 (MQ=255)                                                                                                                                                                                                                          gctTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTcagc     <  1:22905/249‑1 (MQ=255)                                                                                                                                                                                                                           ctTGGCACGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCACCAAAGACTTAGTCAGTAGAAATATGATGAAAGCGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCagcagc  >  1:146454/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CAACATACAACCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCATATGTACGTTTCCACGCGCGGACTAAATGATCGCTGGATGCTTTGGATGCGGAATAAGGGCTGCTTGGCGCGTAAGCTGTCGTCTCAGTAAATAAGGGTAATTCTTCTGTATTATTTACTTCATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGC  >  NC_000913/2112353‑2112821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑