Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,642	T→C	intergenic (+18/‑296)	asnT → / → yeeJ	tRNA‑Asn/inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,642	0	T	C	100.0%	30.0 / NA	9	intergenic (+18/‑296)	asnT/yeeJ	tRNA‑Asn/inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/7);  total (2/7)

TAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTA  >  NC_000913/2044446‑2044874                                                                                                                                                                                                     |                                                                                                                                                                                                                                          taattaaCAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCTCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGATGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTGTAACGGATAGCg                                                                                                                                                                                     <  2:179647/250‑1 (MQ=255)                   attaattaACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgc                                                                                                                                                                  <  1:314301/251‑1 (MQ=255)                               aatGAAATGACTTATGAAATTTAGTGTTGAAAGACAAGGTACGGCTAAGTAATATTCGCCCCGTTCCCACGATGCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGATGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACAGCTTAAGCGCCCTCGACCtt                                                                                                                                                      <  2:281392/249‑1 (MQ=37)                                                          tGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCTCACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGaa                                                                                                                            <  1:131761/250‑1 (MQ=255)                                                                  aaGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAc                                                                                                                   >  2:200571/1‑251 (MQ=255)                                                                             aaGTAATATTCGCCCCTTTCACACGATTCCCCTGTAGTTCAGGCGGCAGAACATCTGAATGTTAATCCGTATGTCACTGGTTCTAGTCCAGTCAGAGGAGCCATATTCTCAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGTACAGTTGTTTTT‑AACGGATAGCGGGTTTTTAACATCTTAAGCGCCCGCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCATCATTTTCCCCt                                                                                                        <  2:177811/251‑1 (MQ=255)                                                                                                     gATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCa                                                                                <  1:200571/251‑1 (MQ=255)                                                                                                                                                                                   aaaTTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGt   >  1:2152/1‑250 (MQ=255)                                                                                                                                                                                   aaaTTCTAAAAATTCGCTCGTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGTGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCATTAATCTCCCCTCTTGCATCTCACACCCAAAACTTCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTAGCAGTa  <  2:193375/251‑1 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                                          TAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACA‑TTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTA  >  NC_000913/2044446‑2044874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑