Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	105.5 / NA	30	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/12);  total (18/12)

ATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATG  >  NC_000913/246523‑246974                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    atatTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                                                                           <  1:277589/251‑1 (MQ=255)   atttattCAGCTTGAATTGTAGCGCAAATGAGCAACAGCTACAATCCATGAGTAATTTAATAAACGGTTGTTTTTACATAATGAACGGAAATATATATGTTAATTTTATAATAATTGTTTAGTAAGTTTGTTCAGAAGCGATCCGGGATACACTGCAACATTAAAGCATTACCATCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtttt                                                                                                                                                                                                         <  1:16575/251‑1 (MQ=255)            gctgtaaTTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTg                                                                                                                                                                                                <  2:85713/246‑1 (MQ=255)                gAATTGTAGCTCAAATGAGCAACAGCGCCACTCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAAATGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCGTTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGcc                                                                                                                                                                                              >  2:152172/1‑249 (MQ=255)                     gTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGTATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAgcgcg                                                                                                                                                                                        <  2:64192/250‑2 (MQ=255)                                  gCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACGGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACatat                                                                                                                                                                          <  1:163186/251‑1 (MQ=255)                                                                     gATGTTTTGTCATAATTAACTGAAAAATATATGTAAATTTAATAATAATGGTTTATTAAGTTTGTTCAGAACCGATCCGGTACACCCTTCAACATTCAAGAATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTGGCCATCGCCACAACATAACATATCTCGTTCAGCTACGCCTCCCGCGAGCGGAGGCaaa                                                                                                                                       <  2:94076/251‑1 (MQ=255)                                                                                aTAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACAGATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGt                                                                                                                            <  1:188880/251‑1 (MQ=255)                                                                                  aaTTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGAAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGTGttt                                                                                                                          >  2:90701/1‑251 (MQ=255)                                                                                   aTTAACTGAAGTATATATGTTAATTTTCTCATAATGGTTTATTAAGTTTGTTCAGAAACAATCCGGGATCCACTGCAAAATTCAAGCATTACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATACTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACTTATCTCTTTCAGCTCCGCCGCCCGCCAGCGGATGCCAAACCGTGCGCGtttt                                                                                                                         >  2:145544/1‑250 (MQ=255)                                                                                          gAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAc                                                                                                                  >  1:234404/1‑251 (MQ=255)                                                                                                                             taAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                                                               >  1:125938/1‑251 (MQ=255)                                                                                                                             taAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                                                               >  1:112781/1‑251 (MQ=255)                                                                                                                                gTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCg                                                                                                                                               >  1:325369/1‑184 (MQ=255)                                                                                                                                gTTTGTTCAGAAACGATCCGGGATACACGGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTAGTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCGTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCg                                                                                                                                               <  2:325369/184‑1 (MQ=255)                                                                                                                                     ttCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                                                                        >  1:135814/1‑250 (MQ=255)                                                                                                                                        aGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGACTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAgg                                                                    >  1:46472/1‑251 (MQ=255)                                                                                                                                                 ccGGGATACACTGAAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCCCATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCCAAACCGTGCGCGTTTATTAAAACAGTACAAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGCAGGCAATGCCg                                                            >  2:293892/1‑250 (MQ=255)                                                                                                                                                          acTGCAACATTAAAGCATGACCAGCCATTATTGAGTCTGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaa                                                    >  2:36958/1‑249 (MQ=255)                                                                                                                                                          acTGCAACATTAAAGAATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaa                                                    >  1:256401/1‑249 (MQ=255)                                                                                                                                                           cTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAgg                                                 >  2:171125/1‑251 (MQ=255)                                                                                                                                                              cAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGttt                                                                                                                          >  2:132897/1‑175 (MQ=255)                                                                                                                                                              cAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGttt                                                                                                                          <  1:132897/175‑1 (MQ=255)                                                                                                                                                                 cATTAAACCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGAACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCt                                            >  2:100592/1‑250 (MQ=255)                                                                                                                                                                                    atcattgattttGTCCTTGCCTTC‑ATTACCATCCTTTGTATGGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCTCTTACAGCCACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGGACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGc                        <  2:256401/240‑1 (MQ=255)                                                                                                                                                                                       attGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTCTTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGc                        >  2:58771/1‑248 (MQ=255)                                                                                                                                                                                                gTCCTTGCCGTCGATT‑ACATCCTTTGTATTGTCTGGATTACTGTTGCTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGAGCGCGTTTATTAAAACAGTACCAAACTCCTCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATACCGAAAGCAAAAGGGCGCTACGACAGCACAACCGGGAGAAGATAAAACAGc            >  2:256796/1‑251 (MQ=255)                                                                                                                                                                                                           cGATT‑CCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATg  <  1:87763/250‑1 (MQ=255)                                                                                                                                                                                                                  cATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCgg                            >  2:160411/1‑218 (MQ=255)                                                                                                                                                                                                                  cATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCgg                            <  1:160411/218‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    ATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATT‑CCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATG  >  NC_000913/246523‑246974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑